Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

159,258 results

Showing: 8051 - 8100 of 159258 results

  2. DsRed2-C1

    Plasmid
    #54611
    Purpose
    Localization: C1 Cloning Vector, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    DsRed2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54611-plasmid-map-sequence-id-257389

    Enlarge

  3. pLenti-CMV-CAG-GFP-EnvA(N2c)

    Plasmid
    #194353
    Purpose
    Lentivirus expressing eGFP and (EnvA-N2c Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies of CVS-N2c strain
    Depositor
    Marco Tripodi
    Article
    Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi
    Inserts
    Enhanced Green Fluorescent Protein (eGFP)
    EnvA-CVS-N2c Rabies Glycoprotein
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194353-plasmid-map-sequence-id-391646

    Enlarge

  4. SpCas9-NRCH-ABE8e(V106W)

    Plasmid
    #198556
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198556-plasmid-map-sequence-id-388914

    Enlarge

  8. RANLS-DEVD-BNES

    Plasmid
    #50840
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Use
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    50840-plasmid-map-sequence-id-76287

    Enlarge

  12. pCWXPGR-pTF-EpoR

    Plasmid
    #114289
    Purpose
    TET-inducible expression of Erythropoietin receptor
    Depositor
    Patrick Salmon
    Insert
    Erythropoietin Receptor (Epor Rat)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114289-plasmid-map-sequence-id-218954

    Enlarge

  13. TFORF2916

    Plasmid
    #143670
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIB (NFIB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143670-plasmid-map-sequence-id-400438

    Enlarge

  17. TFORF1680

    Plasmid
    #142824
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GTF2IRD2B (GTF2IRD2B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142824-plasmid-map-sequence-id-396017

    Enlarge

  19. hsKIF5B(1-560)-HaloTag-FRB

    Plasmid
    #213899
    Purpose
    Imaging of KIF5B fused to HaloTag
    Depositor
    Lukas Kapitein
    Insert
    hsKIF5B(aa1-560)-HaloTag-FRB (KIF5B Human)
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213899-plasmid-map-sequence-id-426209

    Enlarge

  28. TFORF2236

    Plasmid
    #141982
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GATA4 (GATA4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    141982-plasmid-map-sequence-id-386498

    Enlarge

  31. pBABE GFP

    Plasmid
    #10668
    Purpose
    Empty backbone for retroviral gene expression. Select recipient cells using GFP.
    Depositor
    William Hahn
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 11, 2005
    Availability
    Academic Institutions and Nonprofits only
    10668-plasmid-map-sequence-id-106108

    Enlarge

  33. pMZ7

    Plasmid
    #223180
    Purpose
    pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.
    Depositor
    Paul Blainey
    Article
    Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.
    Type
    Empty backbone
    Use
    Chromosomal integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    223180-plasmid-map-sequence-id-441239

    Enlarge

  34. pQE-60 roGFP2-His

    Plasmid
    #65046
    Purpose
    Bacterial expression of His-tagged roGFP2
    Depositor
    Tobias Dick
    Insert
    roGFP2
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    see sequence
    Promoter
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65046-plasmid-map-sequence-id-350692

    Enlarge

  37. pG1K

    Plasmid
    #71741
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71741-plasmid-map-sequence-id-130144

    Enlarge

  39. mCherry-ER-3

    Plasmid
    #55041
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55041-plasmid-map-sequence-id-173833

    Enlarge

  40. pBAD24-sfGFPx2

    Plasmid
    #51559
    Purpose
    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)
    Depositor
    Francisco Malagon, Sankar Adhya
    Article
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    superfolder GFP
    Use
    Tags
    GLESTCRHASLAVLADERRFSA and MARARA
    Expression
    Bacterial
    Mutation
    Contains additonal aa in the N and C terminus (in…
    Promoter
    Available since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    51559-plasmid-map-sequence-id-78831

    Enlarge

  42. TRUPATH Triple (bidirectional) GasS

    Plasmid
    #196055
    Purpose
    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196055-plasmid-map-sequence-id-385866

    Enlarge

  44. TFORF2652

    Plasmid
    #142969
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ARNTL2 (BMAL2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142969-plasmid-map-sequence-id-397337

    Enlarge

  46. PTGER2-Tango

    Plasmid
    #66484
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    PTGER2 (PTGER2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66484-plasmid-map-sequence-id-276276

    Enlarge

  48. TFORF2911

    Plasmid
    #143666
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIC (NFIC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143666-plasmid-map-sequence-id-400498

    Enlarge

  50. piRFP

    Plasmid
    #31857
    Purpose
    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging
    Depositor
    Vladislav Verkhusha
    Article
    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103
    Has Service
    Cloning Grade DNA
    Insert
    iRFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…
    Promoter
    CMV
    Available since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    31857-plasmid-map-sequence-id-396432

    Enlarge

Showing: 8051 - 8100 of 159258 results