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We narrowed to 9,352 results for: UTY

Showing: 8101 - 8150 of 9352 results

  1. PB-TAC-OKMS-KRAB-dCas9

    Plasmid
    #120356
    Purpose
    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OKMS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [10-483]
    Promoter
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120356-plasmid-map-sequence-id-236039

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  2. PB-TAC-OK+9MS-KRAB-dCas9

    Plasmid
    #120357
    Purpose
    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OK+9MS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [1-483]
    Promoter
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120357-plasmid-map-sequence-id-236025

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  9. Antares-SEPLuc C1A4E

    Plasmid
    #141088
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-IRESv24-SEP-C1A4E
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Please see depositor comments
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    141088-plasmid-map-sequence-id-296867

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  12. pSB1C3_mTq-Tat (c-plasmid)

    Plasmid
    #127635
    Purpose
    Encodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.
    Depositor
    Friedrich Simmel
    Article
    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.
    Insert
    mTurquoise2
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PCP and RNA binding peptide:…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    127635-plasmid-map-sequence-id-263753

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  13. pCas9FnCpf1GG

    Plasmid
    #131013
    Purpose
    Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    GFP expression cassette
    direct repeat of FnCas12a
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131013-plasmid-map-sequence-id-257083

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  17. pSG5 N-HA SADBs shRNA resistant

    Plasmid
    #67157
    Purpose
    N-terminally HA tagged mouse SADBs containing substitutions providing resistance to an shRNA
    Depositor
    Maria Alvarado-Kristensson, Ana Carrera
    Article
    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d
    Insert
    Brsk1 (Brsk1 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Nucleotide 294 C-T Nucleotide 297 C-G (shRNA resi…
    Promoter
    SV40 promoter
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

    Plasmid
    #138198
    Purpose
    FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.
    Depositor
    Jin Zhang
    Article
    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi
    Insert
    Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Synthetic, Mouse)
    Use
    Tags
    Cerulean and cpV[E172]
    Expression
    Mammalian
    Mutation
    Contains arginine-to-alanine substitution at resi…
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    138198-plasmid-map-sequence-id-307497

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  37. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid
    #169658
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169658-plasmid-map-sequence-id-365631

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  41. CDK1as_T2A_Zeo

    Plasmid
    #118596
    Purpose
    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.
    Depositor
    William Earnshaw
    Article
    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.
    Insert
    CDK1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    118596-plasmid-map-sequence-id-229650

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  42. pTC-ApoE-Tet-YAP

    Plasmid
    #85580
    Purpose
    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (ApoE.HCR.hAAT promoter) in mouse liver
    Depositor
    Ursula Ehmer
    Article
    Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg
    Inserts
    CreER
    YAP
    Use
    Cre/Lox
    Tags
    Flag (2x)
    Expression
    Mammalian
    Mutation
    Promoter
    ApoE.HCR.hAAT and TRE
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85580-plasmid-map-sequence-id-169837

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  43. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid
    #133779
    Purpose
    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    Isocitrate dehydrogenase 1 (IDH1 Human)
    Use
    Tags
    Myc and FLAG
    Expression
    Mammalian
    Mutation
    Arginine is mutated to histidine at amino acid po…
    Promoter
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133779-plasmid-map-sequence-id-261114

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  44. EGFP in pDBEcR-pIND(SP1)/Bla

    Plasmid
    #201557
    Purpose
    Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR and EGFP
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    DBEcR-myc and FLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pIMD(SP1)
    Available since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201557-plasmid-map-sequence-id-400936

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Showing: 8101 - 8150 of 9352 results