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178,651 results

Showing: 4891 - 4920 of 178651 results

  3. mCherry-NbALFA

    Plasmid
    #159987
    Purpose
    For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)
    Depositor
    Motoyuki Hattori
    Publication
    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:
    Insert
    mCherry-NbALFA
    Tags
    8histidine-HRV3C protease cleavage site
    Expression
    Bacterial
    Mutation
    3GS linker between mCherry and NbALFA
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    159987-plasmid-map-sequence-id-314745

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  5. pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-Puro

    Plasmid
    #141389
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-orf7b (ORF7b SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141389-plasmid-map-sequence-id-288084

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  8. pBT375

    Plasmid
    #125616
    Purpose
    Expresses evoAPOBEC1-BE4max-NG in mammalian cells
    Depositor
    David Liu
    Publication
    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    evoAPOBEC1-BE4max-NG
    Expression
    Mammalian
    Mutation
    E4K H109N H122L D124N R154H A165S P201S F205S
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    125616-plasmid-map-sequence-id-243546

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  10. Tandem p40p35

    Plasmid
    #108665
    Purpose
    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker
    Depositor
    Nevil Singh
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    interleukin-12 (Il12a, Il12b Mouse)
    Expression
    Mammalian
    Mutation
    IL-12 dimers have a Gly-Ser linker
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108665-plasmid-map-sequence-id-260875

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  11. pXPR_047

    Plasmid
    #107145
    Purpose
    measures Cas9 activity
    Depositor
    David Root
    Insert
    GFP + sgRNA for GFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107145-plasmid-map-sequence-id-205295

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  12. pCI-neo Fluc EGFP

    Plasmid
    #90170
    Purpose
    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins
    Depositor
    Franz-Ulrich Hartl
    Publication
    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLuc EGFP
    Expression
    Mammalian
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90170-plasmid-map-sequence-id-226521

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  13. pCI-neo FlucDM EGFP

    Plasmid
    #90172
    Purpose
    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation
    Depositor
    Franz-Ulrich Hartl
    Publication
    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLuc EGFP
    Expression
    Mammalian
    Mutation
    R188Q, R261Q in FLuc
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90172-plasmid-map-sequence-id-226525

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  15. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86613-plasmid-map-sequence-id-168851

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  21. N174-MCS (Puro)

    Plasmid
    #81068
    Purpose
    Lentiviral vector for constitutive gene expression.
    Depositor
    Adam Karpf
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1α
    Available since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    81068-plasmid-map-sequence-id-155844

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  29. mCherry-Rab7a-7

    Plasmid
    #55127
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab7a (RAB7A Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    N117S in NM_004637.5
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55127-plasmid-map-sequence-id-311825

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Showing: 4891 - 4920 of 178651 results