We narrowed to 24,999 results for: CHI

Showing: 8251 - 8300 of 24999 results

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pGC4780

Plasmid

#113408

Purpose

Translation reporter with inducible translation coupling (high native GFP expression)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Insert

158_33121123_1

Use

Synthetic Biology

Tags

fused to codon deptimized sfGFP with Flexible lin…

Expression

Bacterial

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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PCDHGC5-mCherry

Plasmid

#119934

Purpose

Protocadherin gamma C5 expression (mCherry-tag at the C-terminus).

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Tags

mCherry

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-30nmSAH-GFP(1-10)

Plasmid

#61662

Purpose

30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-30nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TRPgamma

Plasmid

#83271

Purpose

Mammalian expression plasmid for Drosophila TRPgamma cDNA

Depositor

Craig Montell

Article

Xu et al Neuron. 2000 Jun;26(3):647-57.

Insert

TRPgamma (Trpgamma Fly)

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pNAS1B split mCherry N-MESVD/TRAP(RK)-C

Plasmid

#61967

Purpose

Duet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-MESVD
TRAP(RK)-C-mCherry

Expression

Bacterial

Promoter

PBAD and PLtetO

Available Since

Feb. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Rac1

Plasmid

#163607

Purpose

Expresses Rac1 Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Rac1 (Rac1 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cHAIAA7-spo5DSR

Plasmid

#49873

Purpose

for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

IAA17 degron tag

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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Cry2PHR - IBAR

Plasmid

#176602

Purpose

Cry2PHR with C-terminal IBAR domain

Depositor

Robert Hughes

Article

Wurz et al J Biol Chem. 2022 Aug 18;298(10):102388.

Insert

Cry2PHR-mCherry-IBAR

Expression

Mammalian

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1

Plasmid

#185571

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1 (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLNL1133

Plasmid

#160864

Purpose

PJ23110-RBS-ScEEB1-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Medium-chain fatty acid ethyl ester synthase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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tdEos-Paxillin-KM-C-10

Plasmid

#57654

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Paxillin KM

Tags

tdEos

Expression

Mammalian

Mutation

Mutation to remove Kozak sequence

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGbH500-Nanog-AXP

Plasmid

#140279

Purpose

a targeting plasmid for Nanog-APEX

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2

Use

Mouse Targeting

Promoter

endogenous

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean3-MannII-N-10

Plasmid

#55429

Purpose

Localization: Mannosidase/Golgi, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

MannII

Tags

mCerulean3

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAK

Plasmid

#19490

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

dnaK molecular chaperone DnaK

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-SEC7-Cerulean

Plasmid

#18851

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

SEC7 (SEC7 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pOGG016

Plasmid

#113990

Purpose

Vector construction module. Endlinker/terminator module to circularize plasmid following Part 6

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Endlinker/terminator Part 6

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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DT043A

Plasmid

#159404

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.T7-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

T7 bacteriophage promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mYFP

Plasmid

#105141

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P41nmt1-mYFP

Tags

41nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFREE2-eSpCas9-sgRNA

Plasmid

#179580

Purpose

Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

Depositor

Kevin Solomon

Article

Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

Insert

eSpCas9

Expression

Bacterial

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pZA57

Plasmid

#174770

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-4xMyc, Kmr plant selection marker

Tags

4xMyc

Expression

Plant

Mutation

WT

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::RpaA D53A

Plasmid

#105229

Purpose

inducible expression of unphosphorylatable variant of RpaA (D53A)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

rpaA driven by Ptrc promoter

Use

Cyanobacteria cloning vector

Mutation

D53 mutated to A

Promoter

ptrc

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pC.059

Plasmid

#119564

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Ptrc10

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pClneoEGFP human RASSF6b

Plasmid

#37021

Depositor

Yutaka Hata

Article

Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Insert

RASSF6b (RASSF6 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2012

Availability

Academic Institutions and Nonprofits only
pLsCas13a-gRNA-1

Plasmid

#164865

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-1

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFUS2_a1a

Plasmid

#50680

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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pBluescript_VEGFA-TS3_SaCas9_sgRNA2

Plasmid

#107330

Purpose

U6 driven SaCas9 sgRNA expression for VEGFA site 3

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

VEGFA-TS3 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pANY3

Plasmid

#112197

Purpose

This plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.

Depositor

Yingfeng An

Article

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB8382

Plasmid

#126916

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pPTK007-3a-αMFΔ

Plasmid

#84966

Purpose

Type 3a, α-mating factor pre-sequence shortened - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor, pre-sequence shortened secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh1 3m shRNA

Plasmid

#122021

Purpose

Negative control shRNA for SEPT11 sh1 shRNA (plasmid 122020).

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
tdEos-FAK-5

Plasmid

#57612

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

FAK

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pZA71

Plasmid

#174785

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker

Tags

3xFLAG

Expression

Plant

Mutation

WT

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA68

Plasmid

#174781

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

L17E/D481V

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA66

Plasmid

#174779

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

D481V

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA55

Plasmid

#174768

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E without STOP codon

Use

Unspecified

Mutation

L17E

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA56

Plasmid

#174769

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V without STOP codon

Use

Unspecified

Mutation

L17E/D481V

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA54

Plasmid

#174767

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V without STOP codon

Use

Unspecified

Mutation

D481V

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA53

Plasmid

#174766

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn without STOP codon

Use

Unspecified

Mutation

WT

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA65

Plasmid

#174778

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

WT

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA67

Plasmid

#174780

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

L17E

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
circRAJ31-Toehold_mutant

Plasmid

#69930

Purpose

Expresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

plTet-circRAJ31_toeholdmutant

Use

Synthetic Biology

Mutation

4 bases mutated in toehold of riboregulator

Promoter

PltetO1-2

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-U6-BssgRNA

Plasmid

#121957

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

BsCas12b single chimeric gRNA

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-U6-AmsgRNA

Plasmid

#121956

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AmCas12b single chimeric gRNA

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPTK008-3a-αMFΔ no Kex

Plasmid

#84967

Purpose

Type 3a, α-mating factor no Kex recognition site - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor no Kex recognition site secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_ZNF503

Plasmid

#135735

Purpose

Donor vector for 3' FLAG tag of human ZNF503

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF503 Homology arms (ZNF503 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Tenomodulin

Plasmid

#19200

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Tenomodulin (TNMD Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
mCerulean3-LC-Myosin-C-10

Plasmid

#55427

Purpose

Localization: Light Chain Myosin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

LC-Myosin

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
SepOTSγ

Plasmid

#68284

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 1xtRNA-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLLFLN

Plasmid

#40030

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

FRT-Lox5171-Lox2272-FRT-loxP
Neo

Expression

Bacterial and Mammalian

Promoter

None and SV40

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 24,999 results for: CHI