We narrowed to 12,882 results for: LIC

1. pDisplay-oROS-HT

   Plasmid

   #216416

   Purpose

   Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   pDisplay-oROS-HT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP

   Plasmid

   #192822

   Purpose

   Golden Gate compatible plasmid with mCherry and Neo

   Depositor

   Mark Denham

   Article

   Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

   Insert

   mCherry and Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC-MLXγ

   Plasmid

   #211755

   Purpose

   Cloning vector for the expression of mouse MLXγ

   Depositor

   Russell Goodman

   Article

   Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

   Insert

   MLX (Mlx Mouse)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-myc-his-BACH1 K52R

   Plasmid

   #17643

   Depositor

   Ronny Drapkin

   Article

   Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

   Insert

   BACH1 (BRIP1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   The lysine residue at position 52 was changed to …

   Promoter

   Available since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

5. CMV-VPR-S-L1-dCjCas9 MiniCAFE

   Plasmid

   #169910

   Purpose

   Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   CMV

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-3XHA-Zmat3

   Plasmid

   #167343

   Purpose

   Expresses HA-tagged Zmat3

   Depositor

   Laura Attardi

   Article

   Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

   Insert

   Zinc finger matrin-type 3 (Zmat3 Mouse)
   

   Use

   Tags

   3HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-FLEX-ArchT-GFP

   Plasmid

   #28307

   Purpose

   Cre-dependent viral expression of ArchT-GFP for neuronal inhibition

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Insert

   ArchT-GFP
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-nEF-dCas9

   Plasmid

   #106430

   Purpose

   Expresses SpdCas9 from the nEF promoter in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   nEF-dCas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTriEx-mVenus-PA-Rac1

   Plasmid

   #22007

   Purpose

   Expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1 (RAC1 Human, Avena sativa (oat))
   

   Use

   Tags

   6X His and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

   Promoter

   CMV, p10

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

10. p413 TEF NAPstar3

    Plasmid

    #232740

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar3 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Seattle_IPDA_control_9_003

    Plasmid

    #167349

    Purpose

    ddPCR gating control for droplets containing either gag+env targets OR tat + env targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    gag_tat_env
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Seattle_IPDA_control_6_002

    Plasmid

    #167348

    Purpose

    ddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    pol_gag_tat
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Seattle_IPDA_control_29_006

    Plasmid

    #167352

    Purpose

    ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    pol_env
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1/NDUFS2/myc-His

    Plasmid

    #127486

    Purpose

    Expresses myc-tagged NDUFS2 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hCXCR4-LSSmOrange

    Plasmid

    #110197

    Purpose

    Encodes for human CXCR4 coding sequence tagged with the LSS-mOrange FP

    Depositor

    Sergi Padilla Parra

    Article

    Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

    Insert

    hCXCR4 (CXCR4 Human)
    

    Use

    Tags

    LSSmOrange

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1/NDUFS7/myc-His

    Plasmid

    #127488

    Purpose

    Expresses myc-tagged NDUFS7 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS7 (NDUFS7 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGART-2XStrep

    Plasmid

    #125681

    Purpose

    expresses human GART protein with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    GART (GART Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-FRT-TO-Nsp13_GC3opt

    Plasmid

    #157714

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

    Plasmid

    #224500

    Purpose

    Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-mNeonGreen-HA-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Tag4A-MMP8 Wt

    Plasmid

    #29545

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pBabe-Puro-IKBalpha-wt

    Plasmid

    #15290

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    inhibitor of Kappa B alpha (NFKBIA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pEF-Bos-FLAG MDA5 MIII

    Plasmid

    #52876

    Purpose

    Expresses MDA5 with a mutation to motif III in mammalian cells

    Depositor

    Curt Horvath

    Article

    Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

    Insert

    IFIH1 (IFIH1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T488A S490A

    Promoter

    Available since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pT2K-LRL-IRES-Luc2

    Plasmid

    #109230

    Purpose

    For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

    Depositor

    Martine Roussel

    Article

    Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

    Insert

    LRL and Luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. plv-AcGFP-SOD1 (G93A)

    Plasmid

    #27142

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    AcGFP

    Expression

    Mutation

    G93A

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

25. Cx32R220stop

    Plasmid

    #78151

    Purpose

    Mammalian Expression of the first 219 amino acids of Connexin 32 and EGFP from a bicistronic mRNA

    Depositor

    Mario Bortolozzi

    Article

    Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

    Insert

    Connexin 32 (GJB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Only contains first 219 residues

    Promoter

    CMV

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

26. pLV302

    Plasmid

    #119943

    Purpose

    Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFPV25-cR-2iG

    Plasmid

    #204618

    Purpose

    Fluorescent reporter for SPI2 induction in Salmonella

    Depositor

    Jacques Neefjes

    Article

    Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

    Inserts

    rpsM promoter
    sseA promoter
    

    Use

    Tags

    GFP and dsRed

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDH-EF1-FHC-POLQ-K121M

    Plasmid

    #64877

    Purpose

    lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M, a mutation in the conserved ATP-binding si…

    Promoter

    EF1alpha

    Available since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV312.3

    Plasmid

    #119944

    Purpose

    Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGK-HELLO-T2A-Cre LV

    Plasmid

    #202003

    Purpose

    Generates lentiviral vector for autochthonous KP-HELLO tumor initiation by intratracheal administration to KP mice

    Depositor

    Nikhil Joshi

    Article

    Cui et al Cell. 2021 Dec 9;184(25):6101-6118.e13.

    Insert

    HELLO-T2A-Cre
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pGK

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p5E-1.7kbfabp6

    Plasmid

    #159087

    Purpose

    p5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene

    Depositor

    John Rawls

    Article

    Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

    Insert

    1.7kb fabp6 promoter (fabp6 Zebrafish)
    

    Use

    Promoter fragment 5' entry vector for tol2 c…

    Tags

    Expression

    Mutation

    Promoter

    1.7kb fabp6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pD2sE_SC14

    Plasmid

    #219717

    Purpose

    DV2 stable E dimer

    Depositor

    Brian Kuhlman

    Article

    Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

    Insert

    DV2 sE SC14
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    A259W, T262R, F279W, T280P

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1/NDUFV2/myc-His

    Plasmid

    #127491

    Purpose

    Expresses myc-tagged NDUFV2 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFV2 (NDUFV2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFRLuc-CoV2-FLucStop

    Plasmid

    #177617

    Purpose

    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).

    Depositor

    Junjie Guo

    Article

    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

    Insert

    SARS-CoV-2 FSE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    In-frame stop codon added between FLuc and FSE

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-HA-PQBP1

    Plasmid

    #25804

    Depositor

    Marius Sudol

    Article

    Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

    Insert

    PQBP1 (PQBP1 Human)
    

    Use

    Tags

    6xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pInducer20 TIFA T9A

    Plasmid

    #231590

    Purpose

    TIFA gene mutated in position T9A (mutation on phosphorylation site) under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    TIFA (TIFA Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    T9A

    Promoter

    doxycycline

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pInducer20 ALPK1 WT

    Plasmid

    #231585

    Purpose

    ALPK1 WT gene under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTRE3G-mouse cGAS-HA

    Plasmid

    #186875

    Purpose

    Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Mouse cGAS with C-terminal HA tag (Cgas Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Gfp-rt-vasa

    Plasmid

    #167322

    Purpose

    Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.

    Depositor

    Goro Yoshizaki

    Article

    Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

    Insert

    rt vasa 3'UTRs (ddx4 rainbow trout)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEN-CAG-mRFP-PCNA pc2729

    Plasmid

    #166040

    Purpose

    Expresses mRFP tagged human PCNA in mammalian cells.

    Depositor

    Cristina Cardoso

    Article

    Heinz et al Nucleic Acids Res. 2018 Jul 6;46(12):611

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. GFP-BLM DM

    Plasmid

    #80071

    Purpose

    Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

    Depositor

    Nathan Ellis

    Article

    Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

    Insert

    BLM (BLM Human)
    

    Use

    Tags

    GFP-BLM

    Expression

    Mammalian

    Mutation

    K317R (AAA>AGA) and K331R (AAA>AGA)

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. p5E-MCS-c-Fos-b-globin (JDW 1237)

    Plasmid

    #229829

    Purpose

    A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    c-fos minimal promoter and b-globin intron
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pIRES2-EGFP-p53 tethered

    Plasmid

    #49245

    Purpose

    expresses two human WT p53s tethered together and also expresses EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53-p53 (TP53 Human)
    

    Use

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Epac-S-H90

    Plasmid

    #170340

    Purpose

    CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

    Insert

    CFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pGLO-GFP-3UAG

    Plasmid

    #82501

    Purpose

    Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescence Protein with 3 UAG codons
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3, …

    Promoter

    AraBAD

    Available since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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46. pRGEN-CMV-VPR-L1-CjCas9 V-D

    Plasmid

    #169913

    Purpose

    Expression of a VPR-dCjCas9 fusion protein to activate gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    dCjCas9
    

    Use

    CRISPR

    Tags

    2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    D8A, H559A in cjCas9

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLL5.0-Anillin shRNA

    Plasmid

    #187270

    Purpose

    Lentiviral expression of shRNA targeting Anillin

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    shRNA for Anillin (ANLN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pX330-mitf

    Plasmid

    #69801

    Purpose

    Used to make mitf knockout porcine cell line

    Depositor

    Jianguo Zhao

    Article

    Wang et al Sci Rep. 2015 Aug 21;5:13348. doi: 10.10

    Inserts

    mitf-sgRNA
    SpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta-Actin and U6

    Available since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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49. RpH-ILV-FRB

    Plasmid

    #225137

    Purpose

    RpH-ILV probe strongly colocalises with lysosomal markers with FRB

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pME Actin Vhh Halo Tag (JDW 1223)

    Plasmid

    #224498

    Purpose

    Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-Halo-Tag
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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