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We narrowed to 15,050 results for: NOL

Showing: 8301 - 8350 of 15050 results

  11. pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid
    #228557
    Purpose
    Expresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus
    Depositor
    Neville Sanjana
    Article
    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103
    Insert
    dRfxCas13d
    Use
    Lentiviral
    Tags
    NLS-HA
    Expression
    Mutation
    R239A/H244A/R858A/H863A
    Promoter
    Available since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    228557-plasmid-map-sequence-id-456689

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  13. pGS-BacA-21232

    Plasmid
    #109177
    Purpose
    Plasmid for expression of C-terminally CBP-3xFLAG-ProteinA-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems
    Depositor
    Monika Golas
    Article
    Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.
    Type
    Empty backbone
    Use
    Tags
    CBP-3xFLAG-ProteinA
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    July 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    109177-plasmid-map-sequence-id-232906

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  15. pPMEL-Nluc-hbb

    Plasmid
    #239862
    Purpose
    Template for in vitro transcription of secreted nanoluciferase, with signal peptide from PMEL, flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Article
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Secreted nanoluciferase, with signal peptide from PMEL (gp100), flanked by human haemoglobin beta 5' and 3' UTRs
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    T7 (included as part of insert)
    Available since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239862-plasmid-map-sequence-id-477837

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  21. pCAGEN_C74_eMagAF_V5_NES

    Plasmid
    #235636
    Purpose
    Encodes fusion protein of ALIBi C-terminal fragment (residues 74-321), eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence (order reversed)
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    ALIBi C-terminal fragment
    Use
    Tags
    eMagAF-V5-NES
    Expression
    Mammalian
    Mutation
    residues 74-321
    Promoter
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    235636-plasmid-map-sequence-id-467579

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  28. pCAGEN_MyrPalm_NES_V5_eMagAF_C74

    Plasmid
    #235639
    Purpose
    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, MyrPalm for targeting to plasma membrane
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    ALIBi C-terminal fragment
    Use
    Tags
    MyrPalm-NES-V5-eMagAF
    Expression
    Mammalian
    Mutation
    residues 74-321
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235639-plasmid-map-sequence-id-467543

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  29. pCAGEN_V5_eMagAF_C74_cMycNLS

    Plasmid
    #235640
    Purpose
    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, cMyc nuclear localization sequence for targeting to nucleus
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    ALIBi C-terminal fragment
    Use
    Tags
    V5-eMagAF and cMycNLS
    Expression
    Mammalian
    Mutation
    residues 74-321
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235640-plasmid-map-sequence-id-467541

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  31. pCAGEN_NES_V5_eMagAF_C74_Sec61b

    Plasmid
    #235637
    Purpose
    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, Sec61b for targeting ALIBi to endoplasmic reticulum
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    ALIBi C-terminal fragment
    Use
    Tags
    NES-V5-eMagAF and Sec61b
    Expression
    Mammalian
    Mutation
    residues 74-321
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235637-plasmid-map-sequence-id-467535

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  38. pCAGEN_NES_V5_GFP_FTA

    Plasmid
    #235633
    Purpose
    Encodes V5-GFP with an N-terminal nuclear export sequence and a C-terminal FLAG-TEV cleavage site-AviTag. Used to express a control substrate for ALIBi.
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    GFP
    Use
    Tags
    FLAG-TEV cleavage site-AviTag and NES-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235633-plasmid-map-sequence-id-467518

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  41. pCAGEN_NES_V5_eMagBF_N73

    Plasmid
    #235635
    Purpose
    Encodes fusion protein of nuclear export sequence, V5, eMagBF (enhanced Magnet, basic, fast), ALIBi N-terminal fragment (residues 1-73) (order reversed)
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    ALIBi N-terminal fragment
    Use
    Tags
    NES-V5-eMagBF
    Expression
    Mammalian
    Mutation
    residues 1-73
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235635-plasmid-map-sequence-id-467511

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  46. GB4635

    Plasmid
    #215263
    Purpose
    Module for the expression of HISPS under the copper inducible CBS:mDFR promoter and H3H under the red light regulated Etr8:CMVmin promoter and the constitutive expression of LUZ, CPH,EGFP and p19
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    CBS4x:mDFR:HISPS-Etr4:mDFR:H3HSF-LUZ-CPH-EGFP-p19
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    215263-plasmid-map-sequence-id-460239

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  48. SpCas9-miniCGBE1

    Plasmid
    #198548
    Purpose
    Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198548-plasmid-map-sequence-id-388891

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  49. SpCas9-NG-miniCGBE1

    Plasmid
    #198538
    Purpose
    Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198538-plasmid-map-sequence-id-388888

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  50. SpCas9-NRRH

    Plasmid
    #198540
    Purpose
    Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRRH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198540-plasmid-map-sequence-id-388897

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Showing: 8301 - 8350 of 15050 results