19,634 results

1. DsRed2-Mito-7

   Plasmid

   #55838

   Purpose

   Localization: Mitochondria, Excitation: 558, Emission: 583

   Depositor

   Michael Davidson

   Insert

   Mito (COX8A Human)
   

   Tags

   DsRed2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. LV-RFP

   Plasmid

   #26001

   Depositor

   Elaine Fuchs

   Article

   Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

   Insert

   H2B-RFP (H2BC21 Human, A. victoria)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF6-Acly(S455D)

   Plasmid

   #70768

   Purpose

   Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Tags

   myc-epitope

   Expression

   Mammalian

   Mutation

   S455D

   Promoter

   EF1alpha

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.GFAP.iGABASnFR

   Plasmid

   #112172

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR
   

   Use

   AAV

   Promoter

   GFAP

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEFIRES-P-ACSL3-mCherry

   Plasmid

   #87158

   Purpose

   Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ACSL3 (ACSL3 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF-1-alpha

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. HRE-luciferase

   Plasmid

   #26731

   Purpose

   Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

   Depositor

   Navdeep Chandel

   Article

   Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

   Insert

   Hypoxia Responsive Elements (3)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti_gRNA-Puro

   Plasmid

   #84752

   Purpose

   Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMMP-MCS-IRES-eGFP-P2A-iCas9

   Plasmid

   #178533

   Purpose

   For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

   Depositor

   Ryoji Yao

   Article

   Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

   Insert

   iCaspase9 (CASP9 Human)
   

   Expression

   Mammalian

   Mutation

   FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Caveolin-C-10

   Plasmid

   #55008

   Purpose

   Localization: Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

   Insert

   Caveolin (CAV1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-N-RAM-d2tTA-TRE-mKate2

    Plasmid

    #140275

    Purpose

    N-RAM, the Npas4-dependent reporter

    Depositor

    Yingxi Lin

    Article

    Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

    Insert

    mKate2
    

    Use

    AAV

    Promoter

    TRE

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9 TadCBEd

    Plasmid

    #193835

    Purpose

    Express TadCBEd (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDd-SpCas9 D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mcherry-DN KASH

    Plasmid

    #125553

    Purpose

    mcherry labeled DN-KASH

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    SYNE1 (SYNE1 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Truncated form: only the KASH domain of nesprin-…

    Promoter

    CMV

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. H2B-Venus

    Plasmid

    #20971

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    Histone cluster 1, H2bb (H2BC3 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Available Since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #191797

    Purpose

    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized dCas13d
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    R239A, H244A, R858A, H863A

    Promoter

    CBh, SV40, hU6

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. LPC-flag-REST-WT

    Plasmid

    #41903

    Depositor

    Stephen Elledge

    Article

    Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

    Insert

    RE1-silencing transcription factor (REST Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

    Plasmid

    #89347

    Purpose

    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    Jurkat TCRalpha/beta alleles and CD3e and CD3z
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PJ-DEAD

    Plasmid

    #38002

    Depositor

    Robin Irvine

    Article

    Hammond et al Science. 2012 Jun 21.

    Inserts

    FKBP1A (FKBP1A Human)
    SAC1 (SAC1 Budding Yeast)
    INPP5E (INPP5E Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Asp 1263 mutated to Ala and Cys 779 mutated to Ser

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. 476-Desmocollin 2-myc

    Plasmid

    #32233

    Depositor

    Kathleen Green

    Article

    Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35

    Insert

    Desmocollin 2 (DSC2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    human beta-actin

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pLV_hEF1a_rtTA3

    Plasmid

    #61472

    Purpose

    3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Insert

    rtTA3
    

    Use

    Lentiviral

    Promoter

    hEF1a

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF6-Acly(S455A)

    Plasmid

    #70767

    Purpose

    Expresses phosphomutant mouse ATP-citrate lyase in mammalian cells

    Depositor

    Kathryn Wellen

    Article

    Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

    Insert

    ATP Citrate Lyase (Acly Mouse)
    

    Tags

    myc-epitope

    Expression

    Mammalian

    Mutation

    S455A

    Promoter

    EF1alpha

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge