We narrowed to 10,458 results for: T7

1. pcDNA3-AMPKsub-YPet-NES

   Plasmid

   #84635

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub-YPet-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CBP TAZ1(340-439)

   Plasmid

   #173760

   Purpose

   bacterial expression of mouse CBP TAZ1 domain

   Depositor

   Peter Wright

   Article

   De Guzman et al Biochemistry. 2005 Jan 18;44(2):490-7. d

   Insert

   CBP TAZ1 (Crebbp Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28b-LanCL2 (6xHis-LanCL2)

   Plasmid

   #73407

   Purpose

   Expresses His tagged LanCL2

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   LanCL2 (LANCL2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pET151-Vps4(101-437)-Hcp1

   Plasmid

   #87737

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100

   Promoter

   T7

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCellFree_G03 BATF3

   Plasmid

   #67050

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   BATF3 (BATF3 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. Dapp1-PH pET11a

   Plasmid

   #71376

   Purpose

   Phosphoinositide Binding

   Depositor

   Mark Lemmon

   Article

   Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

   Insert

   Dapp1-PH (DAPP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 148-273

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pET28-mTDGa.0

   Plasmid

   #81050

   Purpose

   bacterial expression of murine TDG

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TDG (Tdg Mouse)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmCellFree_KA2_ZIKV E

   Plasmid

   #145361

   Purpose

   Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV Envelope
   

   Use

   Tags

   8xHis and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-DUET-GST-eIF4G1-His6, eIF4E

   Plasmid

   #37232

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Inserts

   TIF4631 (TIF4631 Budding Yeast)
   CDC33 (CDC33 Budding Yeast)
   

   Use

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pET28-sfGFP[N150TAA]

    Plasmid

    #164581

    Purpose

    superfolder GFP with N150TAA mutation

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Insert

    sfGFP-150TAA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N150TAA Mutation

    Promoter

    T7

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSFDuet-1_VNp15-DARPinOFF7

    Plasmid

    #182399

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NpT7-5-Erk2

    Plasmid

    #39230

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pET28a_Pro-IL-36α

    Plasmid

    #214343

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36A (IL36A Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21a - SodA

    Plasmid

    #89612

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase A
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Human USP30 (64-502, construct 13, MG-26-82)

    Plasmid

    #110746

    Purpose

    Bacterial expression of human USP30 (construct 13)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-3C-GST-3C

    Expression

    Bacterial

    Mutation

    F348D, M350S, I353E + insertion deletion: 64-178;…

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-At_PRORP2-His

    Plasmid

    #67870

    Purpose

    bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP2 (PRORP2 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-sfGFP-DogTag Loop B

    Plasmid

    #171780

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop B
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Asp1…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTU387

    Plasmid

    #178981

    Purpose

    pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    CRISPR array from Candidatus Scalindua brodae
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCellFree_G03 TGFB1

    Plasmid

    #67080

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TGFB1 (TGFB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pFvL118

    Plasmid

    #64752

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a Gx-NL

    Plasmid

    #169105

    Purpose

    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTW081

    Plasmid

    #64755

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBSFI-myr-akt-S473A

    Plasmid

    #49190

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    S473A

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pETM33_PEX14_Pex14

    Plasmid

    #178484

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    PEX14_Pex14 (PEX14 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT7-th-sgRNA

    Plasmid

    #65565

    Purpose

    in vitro trancription of sgRNA targeting the zebrafish th locus

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    th sgRNA (th Zebrafish)
    

    Use

    In vitro rna transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCA528 SPASTIN , residues 108-200

    Plasmid

    #180628

    Purpose

    Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 108-200

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. K401-iLid

    Plasmid

    #122484

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Matt Thomson

    Article

    Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

    Insert

    Kinesin 1-401 iLid His tag
    

    Use

    Tags

    His6 and iLid

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-401

    Promoter

    T7

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEW4: pET-32a Dot1L(2-386)

    Plasmid

    #124099

    Purpose

    Dot1L(2-386) expression plasmid

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

    Insert

    Dot1L(2-386) (DOT1L Human)
    

    Use

    Tags

    Trx-His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDF-NtXR1AtR2B2NtS1

    Plasmid

    #160879

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcS-S1, rbcX, raf1, raf2, bsd2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-S1: pBAD, chaperones: T7

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYZ_3WJrep_N19_switch

    Plasmid

    #132723

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N19_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCA528 SPASTIN , residues 112-196

    Plasmid

    #180627

    Purpose

    Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 112-196

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a-Tyr-P(11)4

    Plasmid

    #185784

    Purpose

    Encodes enzyme tyrosinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    Tyr-P(11)4
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDF-NtXNtTcR1AtR2NtB2

    Plasmid

    #160889

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-Tc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-Tc: T7, chaperones: T12

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1(+)-sapphireAKAR(T/A)

    Plasmid

    #118465

    Purpose

    Negative-control mutant for sapphireAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR(T/A)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET3a-hUb

    Plasmid

    #61937

    Purpose

    Expression of hUb in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    human ubiquitin (UBC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

36. TALEN1297

    Plasmid

    #32279

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting hey2 (hey2 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. NpT7-5-ERK1

    Plasmid

    #39229

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    ERK1 (MAPK3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA3-PKCsub-YPet-NES

    Plasmid

    #138207

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKCsub-YPet-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmCellFree_KA2_ZIKV C

    Plasmid

    #145359

    Purpose

    Cell-free/mammalian expression vector for Zika virus capsid protein with N-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV Capsid
    

    Use

    Tags

    8xHis and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCellFree_G03 TFAM

    Plasmid

    #67064

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFAM (TFAM Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    S115C

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only