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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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159,111 results

Showing: 6721 - 6760 of 159111 results

  6. pLKO-Tet-ON-shCtrl (neo)

    Plasmid
    #192076
    Purpose
    Lentivirus for Dox-inducible expression of a non-targeting control shRNA
    Depositor
    Roland Friedel
    Insert
    non-targeting randomized sequence
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    H1 / Tet-responsive element
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192076-plasmid-map-sequence-id-379241

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  9. pCW57-MCS1-P2A-MCS2 (Blast)

    Plasmid
    #80921
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80921-plasmid-map-sequence-id-234619

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  14. pHB-3

    Plasmid
    #140956
    Purpose
    For PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.
    Depositor
    Kang Zhou
    Article
    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.
    Insert
    phaCAB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    140956-plasmid-map-sequence-id-334291

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  16. pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

    Plasmid
    #125256
    Purpose
    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection
    Depositor
    Roger D. Kamm
    Article
    Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi
    Insert
    hChR2(H134R)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-forward
    Available since
    May 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    125256-plasmid-map-sequence-id-245091

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  20. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

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  21. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197026
    Purpose
    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enOsCas12f1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D52R / T132R
    Promoter
    CBh, CMV, hU6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197026-plasmid-map-sequence-id-394112

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  23. U6-5'+3' MS2-CircRNA

    Plasmid
    #213752
    Purpose
    For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Insert
    5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213752-plasmid-map-sequence-id-422189

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  25. BRD4

    Plasmid
    #38943
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD4 (BRD4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38943-plasmid-map-sequence-id-456555

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  26. SpCas9-NG-YE1-BE4max

    Plasmid
    #198535
    Purpose
    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198535-plasmid-map-sequence-id-388892

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  28. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187957
    Purpose
    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187957-plasmid-map-sequence-id-373706

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  31. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid
    #129593
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Use
    Tags
    6xHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129593-plasmid-map-sequence-id-254271

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  35. PM-RA

    Plasmid
    #211705
    Purpose
    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (RA) fused with plasma membrane (PM) targetting motif of Lck in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    PM-targetted RA (Lck Synthetic)
    Use
    Tags
    RA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211705-plasmid-map-sequence-id-419227

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Showing: 6721 - 6760 of 159111 results