We narrowed to 13,374 results for: FLU

1. mTFP1-H2A-10

   Plasmid

   #55488

   Purpose

   Localization: Nucleus/Histones, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   H2A (H2AC13 Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pVH617

   Plasmid

   #169606

   Purpose

   Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (A1-pA::PmPGK1-Fluc-pA::PCREm4-Nluc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PCREm4 / PPGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

   Plasmid

   #192380

   Purpose

   To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mPlum-Gamma-Tubulin-17

   Plasmid

   #55971

   Purpose

   Localization: Centrosomes, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Gamma-Tubulin (TUBG1 Human)
   

   Use

   Tags

   mPlum

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1-P2X2-CFP

   Plasmid

   #22401

   Depositor

   Henry Lester

   Article

   Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

   Insert

   ATP-gated P2X channel 2 (P2rx2 Rat)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

6. EYFP-Clathrin-19

   Plasmid

   #56584

   Purpose

   Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Clathrin (CLTB Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. mVenus-CENPB-N-22

   Plasmid

   #56325

   Purpose

   Localization: Nucleus/Centromeres, Excitation: 515, Emission: 528

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   CENPB (CENPB Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   aa 1-169 of CENPB (DNA Binding Domain)

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. LifeAct-TagBFP-DiB-RM

   Plasmid

   #168782

   Purpose

   Actin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RM

   Depositor

   Jens Meiler

   Article

   Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

   Insert

   LifeAct-TagBFP-DiB-RM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. R10-3 /pQE-30

   Plasmid

   #79859

   Purpose

   The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.

   Depositor

   Konstantin Lukyanov

   Article

   Mishin et al Biochemistry. 2008 Apr 22;47(16):4666-73

   Insert

   R10-3
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Aequorea victoria green fluorescent protein (avGF…

   Promoter

   T5 promoter / lac operator

   Available since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD-SPW

    Plasmid

    #190628

    Purpose

    Bacterial expression of genetically encoded protease indicator SPW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SPW
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. tdEos-Vimentin-N-18

    Plasmid

    #57690

    Purpose

    Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    NM_003380.3 (VIM Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pCDNA-SCW

    Plasmid

    #190633

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBAD-SKW

    Plasmid

    #190631

    Purpose

    Bacterial expression of genetically encoded potassium indicator SKW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SKW
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD-SCW

    Plasmid

    #190626

    Purpose

    Bacterial expression of genetically encoded calcium indicator SCW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVH615

    Plasmid

    #169604

    Purpose

    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (A1-pA::PCREm4-Nluc-pA::PmPGK1-Fluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mTFP1-Tubulin-6

    Plasmid

    #55512

    Purpose

    Localization: Microtubules, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pNB655

    Plasmid

    #60142

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A4V

    Promoter

    MET17pr

    Available since

    Feb. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET27b-ecDHFR-N23PP/S148A

    Plasmid

    #110829

    Purpose

    Bacterial expression of E.coli DHFR N23PP/S148A mutant

    Depositor

    Stephen Benkovic

    Article

    Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23; a proline residue inserted be…

    Promoter

    T7

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTrc99S-ssDsbA-PD-4xDQNAT

    Plasmid

    #128399

    Purpose

    Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    H. influenzae protein D
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTFP1-Rab4a-7

    Plasmid

    #55507

    Purpose

    Localization: Ras GTPase, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mTFP1-H4-23

    Plasmid

    #55491

    Purpose

    Localization: Nucleus/Histones, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    H4 (HIST1H4J Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

22. mTFP1-Alpha-Actinin-19

    Plasmid

    #55464

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pRSET A-pcStar

    Plasmid

    #226339

    Purpose

    Bacterial expression of RSFP pcStar; photoconvertible red fluorescent protein with high brightness, high photoconversion contrast, and early detectable fluorescence

    Depositor

    Pingyong Xu

    Article

    Zhang et al Nano Lett. 2020 Apr 8;20(4):2197-2208. d

    Insert

    pcStar
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ

    Plasmid

    #192399

    Purpose

    Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-Rluc-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ

    Plasmid

    #192398

    Purpose

    On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-Rluc-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pOP004

    Plasmid

    #185985

    Purpose

    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator

    Depositor

    Georgi Belogurov

    Article

    Huang et al Methods Enzymol. 2022;675:207-233. doi:

    Insert

    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

    Plasmid

    #192375

    Purpose

    To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-35Ster-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

    Plasmid

    #192374

    Purpose

    To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCStrunc-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

    Plasmid

    #192385

    Purpose

    To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-OCS-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

    Plasmid

    #192386

    Purpose

    To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

    Plasmid

    #192404

    Purpose

    On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

    Plasmid

    #192401

    Purpose

    Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-Rluc-B3RT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA-RCC

    Plasmid

    #190635

    Purpose

    Mammalian expression of genetically encoded calcium indicator RCC

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    RCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

    Plasmid

    #192400

    Purpose

    On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-Rluc-B3RT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

    Plasmid

    #192402

    Purpose

    On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    FRT-Act2-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

    Plasmid

    #192405

    Purpose

    Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ

    Plasmid

    #192403

    Purpose

    Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    FRT-Act2-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

    Plasmid

    #192381

    Purpose

    To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

    Plasmid

    #192379

    Purpose

    To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

    Plasmid

    #192377

    Purpose

    To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

    Plasmid

    #192378

    Purpose

    To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA5/FRT/TO_H2B-STf

    Plasmid

    #181998

    Purpose

    CMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    H2B-STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCDNA-SCcpV

    Plasmid

    #190636

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCcpV

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCcpV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pOP005

    Plasmid

    #186030

    Purpose

    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoter

    Depositor

    Georgi Belogurov

    Article

    Huang et al Methods Enzymol. 2022;675:207-233. doi:

    Insert

    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pVH616

    Plasmid

    #169605

    Purpose

    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pVH614

    Plasmid

    #169603

    Purpose

    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::PCREm4-Nluc-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR

    Plasmid

    #157672

    Purpose

    Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
    

    Use

    Tags

    ddFLN4, ELP, Histag and ybbR tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET27b-ecDHFR-N23PP

    Plasmid

    #110828

    Purpose

    Bacterial expression of E.coli DHFR N23PP mutant

    Depositor

    Stephen Benkovic

    Article

    Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23; a proline residue inserted be…

    Promoter

    T7

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. mTFP1-SiT-N-15

    Plasmid

    #55508

    Purpose

    Localization: Golgi TGN, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    SiT (ST6GAL1 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    aa 1-45 of SiT

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

50. mTFP1-Talin-ABM-10

    Plasmid

    #55510

    Purpose

    Localization: Talin Actin Binding Domain, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Talin ABM
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Actin Binding Module; I/LWEQ = 197 amino acids fr…

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only