4,178 results

1. pcDNA3-FLAG-RIPK1

   Plasmid

   #78842

   Purpose

   To overexpress RIPK1 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT3 p38

   Plasmid

   #12658

   Depositor

   John Kyriakis

   Insert

   p38alpha (MAPK14 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Sept. 21, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDECKO_mCherry

   Plasmid

   #78534

   Purpose

   Backbone to clone single or paired sgRNAs

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (for expressing sgRNA)

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEX-PK-hLC3

   Plasmid

   #61458

   Purpose

   Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy

   Depositor

   Isei Tanida

   Article

   Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

   Insert

   PK-hLC3 (MAP1LC3B Human, Fission Yeast)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-PIPK1 gamma 90

   Plasmid

   #22299

   Depositor

   Pietro De Camilli

   Article

   Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

   Insert

   human phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMA122 - peel-1 negative selection

   Plasmid

   #34873

   Purpose

   Negative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   peel-1 (peel-1 Nematode)
   

   Use

   Worm targeting

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest-eGFP-N1

   Plasmid

   #31796

   Depositor

   Robin Shaw

   Article

   Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

   Plasmid

   #20943

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CaMKIIa

   Available Since

   July 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. hATMS1981A

   Plasmid

   #32300

   Depositor

   Michael Kastan

   Article

   Bakkenist et al Nature. 2003 Jan 30;421(6922):499-506.

   Insert

   ATM (ATM Human)
   

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   S1981A

   Available Since

   Nov. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Peredox-mCherry-NLS

    Plasmid

    #32384

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry-NLS
    

    Tags

    Nuclear Localization Seq

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCVhygro-F-G9a

    Plasmid

    #41721

    Purpose

    Retroviral expression of human G9a in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

    Insert

    G9a (EHMT2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC

    Plasmid

    #52985

    Purpose

    split Venus attached to MCP and PCP respectively

    Depositor

    Robert Singer

    Article

    Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

    Insert

    nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
    

    Use

    Lentiviral

    Promoter

    ubc

    Available Since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Gamma-Tubulin-17

    Plasmid

    #55050

    Purpose

    Localization: Centrosomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Gamma-Tubulin (TUBG1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMJ920

    Plasmid

    #42234

    Depositor

    Jennifer Doudna

    Article

    Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    GFP, HA epitope, and NLS (nuclear localization si…

    Expression

    Mammalian

    Mutation

    codon-optimized synthetic DNA sequence

    Promoter

    CMV

    Available Since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti CMV/TO Hygro DEST (693-2)

    Plasmid

    #17291

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRc/RSV-m CBP-HA

    Plasmid

    #16701

    Depositor

    Richard Goodman

    Article

    Chrivia et al Nature. 1993 Oct 28. 365(6449):855-9.

    Insert

    CBP (Crebbp Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-TRIM28

    Plasmid

    #45568

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    G34S mutation in EGFP

    Promoter

    CMV

    Available Since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCS6

    Plasmid

    #55752

    Purpose

    Produces T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    T7 RNA Polymerase
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-EGFP-C1-HsNot1

    Plasmid

    #37370

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot1 (CNOT1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. Rictor_2 shRNA

    Plasmid

    #1854

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Rictor (RICTOR Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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