We narrowed to 10,458 results for: T7

1. pcDNA3-mCherry-TSC2-NLS

   Plasmid

   #192441

   Purpose

   Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2-NLS (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TAL2261

   Plasmid

   #36700

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   BMPR1A-TALEN_Right (BMPR1A Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

3. TAL2260

   Plasmid

   #36699

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   BMPR1A-TALEN_Left (BMPR1A Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pET28a-baPrs-hdNadV

   Plasmid

   #91950

   Purpose

   pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

   Depositor

   George Cătălin Marinescu

   Article

   Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

   Inserts

   Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
   Phosphoribosyl Pyrophosphate Synthetases
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Leucine 135 to Isoleucine

   Promoter

   T7

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHis-TEV-UBC-9

   Plasmid

   #87055

   Purpose

   Expresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORF

   Depositor

   Ronald Hay

   Article

   Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

   Insert

   UBC-9 (ubc-9 Nematode)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETSUMO-TtAgocatdead

   Plasmid

   #210322

   Purpose

   Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

   Depositor

   Phillip Zamore

   Article

   Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

   Insert

   TtAgo (TT_P0026 Thermus thermophilus)
   

   Use

   Tags

   6x His tag and SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21a - APX

   Plasmid

   #89618

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Ascorbate dependent Tryparedoxin peroxidase
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. bdSUMO-HA2-GFP-HiBit

   Plasmid

   #171017

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-HA2-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-PLS-KP6_rpoA-C

   Plasmid

   #190966

   Purpose

   Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTK102

    Plasmid

    #110574

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    T7 promoter with RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCDF-NtXNtT2R1AtR2NtB2

    Plasmid

    #160886

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-T2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-T2: T7, chaperones: T9

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Foxn1-pBSII SK

    Plasmid

    #41032

    Depositor

    Nancy Manley

    Article

    Gordon et al Mech Dev. 2001 May;103(1-2):141-3.

    Insert

    Foxn1 in situ probe (Foxn1 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7 sense; T3 antisense

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. His-TEV-GB1 Leu5Met

    Plasmid

    #128686

    Purpose

    Expresses GB1 Leu5Met mutant

    Depositor

    Sharon Rozovsky

    Article

    Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

    Insert

    spg
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pBSFI-myr-akt-T308A

    Plasmid

    #49189

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    T308A

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

15. E. coli 16S toehold switch sensor

    Plasmid

    #110698

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    E. coli 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCellFree_KA2_ZIKV PrM

    Plasmid

    #145360

    Purpose

    Cell-free/mammalian expression vector for Zika virus PrM protein with N-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV PrM
    

    Use

    Tags

    8xHis and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    March 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCellFree_G03 ATF1

    Plasmid

    #67065

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ATF1 (ATF1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET14b_tev_PSGU

    Plasmid

    #59738

    Purpose

    Expresses a.a. 417-887 of human ZO-1

    Depositor

    Alan Fanning

    Article

    Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

    Insert

    zonula occludens-1 (TJP1 Human)
    

    Use

    Tags

    HIS tag and thrombin clvg

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pET28a-ybbr-HIS-ddFLN4-K10

    Plasmid

    #101718

    Purpose

    K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    ddFLN4 fingerprint, K10 peptide (KRT10 Human, Dictyostelium discoideum)
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET16b-Trx C35S

    Plasmid

    #36947

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin-1 C35S mutation (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    cys 35 to serine

    Promoter

    T7

    Available since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pmCellFree_KA2_ZIKV NS4B

    Plasmid

    #145367

    Purpose

    Cell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS4B
    

    Use

    Tags

    8xHis and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-blueCKAR(T/A)

    Plasmid

    #118473

    Purpose

    Negative-control mutant for blueCKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueCKAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Affibody EGFR:1907.BirA*-pET301/NT-DEST

    Plasmid

    #174691

    Purpose

    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*

    Depositor

    Jean Gariepy

    Article

    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

    Insert

    Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
    

    Use

    Tags

    HIS tag

    Expression

    Bacterial

    Mutation

    R118G mutation in BirA

    Promoter

    T7 promoter

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-hDcp2 E147Q, E148Q

    Plasmid

    #72215

    Purpose

    bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E147Q, E148Q

    Promoter

    T7

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pRSETb-rsGreen1

    Plasmid

    #78180

    Purpose

    Expresses rsGreen1 in E. coli strains.

    Depositor

    Peter Dedecker

    Article

    Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

    Insert

    rsGreen1
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pmCellFree_KA1_ZIKV NS2B

    Plasmid

    #145344

    Purpose

    Cell-free/ mammalian expression vector for Zika virus NS2B protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS2B
    

    Use

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pmCellFree_KA1_ZIKV NS4B

    Plasmid

    #145347

    Purpose

    Cell-free/ mammalian expression vector for Zika virus NS4B protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS4B
    

    Use

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-JNKAR1-NES

    Plasmid

    #84632

    Purpose

    Unimolecular, FRET-based JNK activity reporter; cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ECFP-FHA1-JNKsubDD-Citrine-NES
    

    Use

    Tags

    6xHis, Citrine, ECFP, Nuclear export signal (NES)…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TN-XXL pRSETB

    Plasmid

    #45796

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

    Insert

    TN-XXL
    

    Use

    Tags

    6xHIS, ECFP, and YFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pET-30a(+)-Syk-tSH2-PM

    Plasmid

    #111271

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y130E mutation to mimic phosphorylation

    Promoter

    T7 promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TTR C10S S85C K80D

    Plasmid

    #184743

    Purpose

    bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S, S85C, K80D

    Promoter

    T7

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pETM6-At4CL-CmCHS-CmCHI

    Plasmid

    #73395

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    CmCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJK_ToeRepG2_N02_trigger

    Plasmid

    #132745

    Purpose

    T7 RNAP-driven expression of second-generation toehold repressor index 2 trigger RNA

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRepG2_N02_trigger
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pIVT/Smarcc1

    Plasmid

    #122145

    Purpose

    For making Smarcc1 RNA

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Smarcc1 (Smarcc1 Mouse)
    

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGST-GstZ1

    Plasmid

    #163090

    Purpose

    Expressing mouse Gstz1 in bacterial cells

    Depositor

    Joseph Takahashi

    Article

    Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

    Inserts

    GstZ1
    GstZ1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28a-TmCA-P(11)4

    Plasmid

    #185783

    Purpose

    Encodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    TmCA-P(11)4
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. B. fragilis 16S toehold switch sensor

    Plasmid

    #110696

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    B. fragilis 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHis-hPim1

    Plasmid

    #100722

    Purpose

    Expression of human Pim1 kinase domain in E.coli

    Depositor

    Byung Il Lee

    Article

    Lee et al PLoS One. 2013 Jul 31;8(7):e70358. doi:

    Insert

    Pim1 (PIM1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Residues 29-313 of isoform 2

    Promoter

    T7 promotor

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pIVT-EGFP-mouseCENP-C

    Plasmid

    #174444

    Purpose

    in vitro transcription template for mouse CENP-C (N-ter EGFP tagged)

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Centromere protein C
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    T7 SP6

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET42b-A3A-PBE-ΔUGI

    Plasmid

    #119771

    Purpose

    To express the recombinant APOBEC3A in vitro

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS
    

    Use

    Tags

    6*HIS and NLS

    Expression

    Bacterial

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge