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Plasmid#163132PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only
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pYZ_3WJrep_N11_trigger
Plasmid#132733PurposeT7 RNAP-driven expression of 3WJ repressor index 11 trigger RNADepositorInsert3WJ_N11_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a - SodB
Plasmid#89613PurposeExpresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG inductionDepositorInsertSuperoxide dismutase B
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pET-PLS-KP2_rpoA-U
Plasmid#190956PurposeTest the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP2
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6-A66S_rpoA-U
Plasmid#190969PurposeTest the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP3_rpoA-U
Plasmid#190960PurposeTest the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP3
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6-E67A_rpoA-U
Plasmid#190973PurposeTest the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
B. fragilis mRNA toehold switch sensor
Plasmid#110706PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertB. fragilis species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHlip-GFP-HiBit
Plasmid#171016PurposeFor bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHlip-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-triple mutation-pa-GCaMP6f
Plasmid#67570Purposebacterial expression of a photoactivatable fluorescent calcium reporterDepositorInsertpa-GCaMP6f
UseTagsHIS-tagExpressionBacterialMutationV115H, L64F, T65SPromoterT7Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-Gli1
Plasmid#87225PurposeExpress hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coliDepositorInsertGLI1 (Gli1 Mouse)
UseTagshisSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreenF
Plasmid#78181PurposeExpresses rsGreenF in E. coli strains.DepositorInsertrsGreenF
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
p158
Plasmid#62617PurposeePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pmCellFree_KA2_ZIKV NS2A
Plasmid#145363PurposeCell-free/mammalian expression vector for Zika virus NS2A protein with N-term mCherryDepositorInsertZIKV NS2A
UseTagsHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-6xHis-ARL2
Plasmid#67474PurposeExpression of hArl2 with N-term His6 tag in E. coliDepositorInsertARL2 (ARL2 Human)
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 FYN
Plasmid#67106PurposeStandard for cell-free expression benchmarking.DepositorInsertFYN (FYN Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-CH2 (1192-1318)
Plasmid#99338PurposeBacterial expression of mouse CBP CH2 domainDepositorInsertCBP CH2 (1192-1318) (Crebbp Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse eIF2C4
Plasmid#50356PurposeMouse eIF2C4 cDNA cloneDepositorInserteIF2C4 (Ago4 Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-PhCHS-MsCHI
Plasmid#73392PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
PhCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCellFree_G03 CREBZF
Plasmid#67070PurposeStandard for cell-free expression benchmarking.DepositorInsertCREBZF (CREBZF Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR-Kras
Plasmid#84637PurposeUnimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targetedDepositorInsertCerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, C…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Vv4CL-CmCHS-MsCHI
Plasmid#73402PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsVv4CL
CmCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
kank2_pJet1.2
Plasmid#121980Purposefor generating kank2 riboprobes for WISH in zebrafishDepositorInsertkank2 (LOC571549 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CSNK2A1
Plasmid#67085PurposeStandard for cell-free expression benchmarking.DepositorInsertCSNK2A1 (CSNK2A1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)
Plasmid#108195PurposeExpresses PdR-PCNA3(R112C/T180C) fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C) fusion protein
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
C. difficile 16S toehold switch sensor
Plasmid#110704PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertC. difficile 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsubDD-Venus-NES
Plasmid#84630PurposeEncodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsubDD-Venus-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b-At 14-3-3 nu
Plasmid#44940DepositorInsert14-3-3 nu (GRF7 Mustard Weed)
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-G51PEKN
Plasmid#118582PurposeBacterial expression of E.coli DHFR G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationG51 mutated to P51; Glu-Lys-Asn residues inserted…PromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP/S148A
Plasmid#110829PurposeBacterial expression of E.coli DHFR N23PP/S148A mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23; a proline residue inserted be…PromoterT7Available sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG pET
Plasmid#8494DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsFLAG and HisExpressionBacterialMutationFLAG epitope tag replaces T7 tag in the pET back…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 8, MG-26-21)
Plasmid#110745PurposeBacterial expression of human USP30 (construct 8)DepositorInsertUSP30 (USP30 Human)
UseTagsN-His6-3C-GST-3CExpressionBacterialMutationF348D, M350D, I353E + insertion deletion: 64-357;…PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT5R1AtR2NtB2
Plasmid#160888PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T5
UseTagsExpressionBacterialMutationPromoterrbcS-T5: T7, chaperones: T11Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT1R1AtR2NtB2
Plasmid#160885PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T1
UseTagsExpressionBacterialMutationPromoterrbcS-T1: T7, chaperones: T8Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP3-His
Plasmid#67871Purposebacterial expression of PRORP3 (At4g21900), full coding sequence + C-term. His tagDepositorInsertPRORP3 (PRORP3 Mustard Weed)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree _KA2_ZIKV NS5
Plasmid#145368PurposeCell-free/mammalian expression vector for Zika virus NS5 protein with N-term mCherryDepositorInsertZIKV NS5
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSKB3-SaGGR-I206F-L377H (JBEI-7202)
Plasmid#110152PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant geneDepositorInsertGGR
UseTagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-I206F-L377H mutant genePromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only