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We narrowed to 18,759 results for: nls

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Showing: 6761 - 6800 of 18759 results

  5. dABE

    Plasmid
    #124447
    Purpose
    Expresses dead Cas9 (dCas9)-ABE7.10
    Depositor
    George Church
    Publication
    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dABE7.10
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124447-plasmid-map-sequence-id-241059

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  6. dCBE4-gam

    Plasmid
    #124449
    Purpose
    Expresses dead Cas9 (dCas9)-CBE4-gam
    Depositor
    George Church
    Publication
    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCBE4-gam
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124449-plasmid-map-sequence-id-241051

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  9. pLenti spCas9 T2A iRFP670 P2A puro

    Plasmid
    #122182
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    iRFP670
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Available since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122182-plasmid-map-sequence-id-237289

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  10. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid
    #121173
    Purpose
    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
    Depositor
    Timo Otonkoski
    Insert
    dCas9-VP192-T2A-EGFP
    Use
    CRISPR and Lentiviral; Inducible by doxycycline
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121173-plasmid-map-sequence-id-234995

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  13. pV1025

    Plasmid
    #111424
    Purpose
    Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)
    Depositor
    Gerald Fink
    Publication
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111424-plasmid-map-sequence-id-212243

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  15. pCAGGS-mTagBFP2-T2A-iCre

    Plasmid
    #113837
    Purpose
    Monomeric TagBFP2 and iCre recombinase
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCre
    Expression
    Mammalian
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    113837-plasmid-map-sequence-id-222246

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  21. pUBC_V5_MS2-mVenus

    Plasmid
    #107606
    Purpose
    Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag
    Depositor
    John Rinn
    Publication
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2-mVenus
    Use
    Synthetic Biology
    Tags
    V5 and mVenus
    Mutation
    MS2: V75E/A81G
    Promoter
    human UBC
    Available since
    May 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107606-plasmid-map-sequence-id-209713

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  23. pDB4281

    Plasmid
    #98700
    Purpose
    A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system
    Depositor
    Li-Lin Du
    Publication
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98700-plasmid-map-sequence-id-182829

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  24. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100899-plasmid-map-sequence-id-195324

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  26. iChr2-Notch Mosaic (SO250)

    Plasmid
    #99752
    Purpose
    Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins and Notch signalling genes
    Depositor
    Rui Benedito
    Publication
    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do
    Insert
    HIs-H2B-Cherry, H2B-EGFP-V5, HA-H2B-Cerulean, DN-Maml1, NICD-PEST
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Mammalian
    Available since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99752-plasmid-map-sequence-id-182454

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  29. EKARet-nuc

    Plasmid
    #96896
    Purpose
    FLIM sensor for ERK activation in nucleus
    Depositor
    Ryohei Yasuda
    Publication
    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLIM Sensor for ERK Activity in Nucleus
    Tags
    EGFP and sREAChet
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    96896-plasmid-map-sequence-id-182390

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  31. pY117 (pcDNA3.1-huMb3Cpf1)

    Plasmid
    #92293
    Purpose
    Expression of humanized Mb3Cpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huMb3Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92293-plasmid-map-sequence-id-181495

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  39. pCMV-dCas9-mD3A

    Plasmid
    #78257
    Purpose
    Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoter
    Depositor
    Grant Challen
    Publication
    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A CD aa 598-912 (DNMT3A Human)
    Use
    CRISPR
    Tags
    Flag epitope tag
    Expression
    Mammalian
    Mutation
    E756A
    Promoter
    pCMV
    Available since
    June 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78257-plasmid-map-sequence-id-150189

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Showing: 6761 - 6800 of 18759 results