We narrowed to 7,519 results for: Lif

1. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

   Plasmid

   #127789

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in His2…

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH-HT_CtCHAD-R385A-K386A-K389A-K390A

   Plasmid

   #127792

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in Arg3…

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBMN mCherry-NBR1(F562A/Y731A/ΔUBD)

   Plasmid

   #119692

   Purpose

   For mammalian expression of LIR double mutant NBR1 without its UBD

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   NBR1 (NBR1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F562A/Y731A & UBD deletion (1-906 ), See depo…

   Promoter

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBMN iRFP670-OPTN(F178A/D474N)

   Plasmid

   #119694

   Purpose

   For mammalian expression of iRFP-tagged LIR/UBD double mutant OPTN

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   OPTN (OPTN Human)
   

   Use

   Retroviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   F178A/D474N

   Promoter

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL15

   Plasmid

   #117430

   Purpose

   Expression of ARL2

   Depositor

   Richard Kahn

   Article

   Clark et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

   Insert

   ARL2 (ARL2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19_4xT790_28bp

   Plasmid

   #114720

   Purpose

   4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standard

   Depositor

   Nils Walter

   Article

   Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19_T790_28bp

   Plasmid

   #114722

   Purpose

   28 bp fragment of the WT EGFR allele used as SiMREPS analyte standard

   Depositor

   Nils Walter

   Article

   Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PAK4-3'UTR-BS2

   Plasmid

   #113091

   Purpose

   Luciferase reporter containing PAK4 3' UTR with the second predicted MiR-199a-3p binding

   Depositor

   James Donahue

   Article

   Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

   Insert

   p21 Activated kinase 4 (PAK4 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   PGK

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PAK4-3'UTR-BS1

   Plasmid

   #113090

   Purpose

   Luciferase reporter containing PAK4 3' UTR with the first predicted MiR-199a-3p binding site

   Depositor

   James Donahue

   Article

   Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

   Insert

   p21 Activated kinase 4 (PAK4 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   PGK

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. NET39 Short pmRFP-N2 (451)

    Plasmid

    #61981

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET39 (PPAPDC3 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Short form of NET39

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pGL3-PIM2mut3

    Plasmid

    #53742

    Purpose

    promoter region of PIM2 lacking the 2 forkhead binding sites

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    PIM2 (PIM2 Human)
    

    Use

    Luciferase; Reporter vector

    Tags

    Expression

    Mutation

    deletion of 2 forkhead binding sites (bp# 395-497…

    Promoter

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pLVX-Puro-TDP-43-Q331K

    Plasmid

    #133761

    Purpose

    Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed glutamine 331 to lysine.

    Promoter

    CMV

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LAMP1-RpHLuorin2

    Plasmid

    #171720

    Purpose

    Lysosomal expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-Puro-TDP-43-A315T

    Plasmid

    #133755

    Purpose

    Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed alanine 315 to threonine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-Puro-TDP-43-M337V

    Plasmid

    #133759

    Purpose

    Lentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed methionine 337 to valine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. OTR untagged

    Plasmid

    #208913

    Purpose

    encodes the human oxytocin receptor, without any tags

    Depositor

    Christian Gruber

    Article

    Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

    Insert

    OXTR (OXTR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TM5: 5x56, A218, Threonin instead of Alanin

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSPIH6

    Plasmid

    #190676

    Purpose

    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

    Depositor

    John Vederas

    Article

    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

    Inserts

    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    

    Use

    Tags

    Chitin binding domain, His tag, and N-terminal Hi…

    Expression

    Bacterial

    Mutation

    Added C-terminal his tag and None

    Promoter

    T7

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEasiLV-MCS-coICP0fxe

    Plasmid

    #235546

    Purpose

    To express ICP0-FXE (ICP0 ∆106-149, doxycycline inducible)

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    ICP0
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of the RING domain, ∆106-149.

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-EFS-foldon-GFP- PCP

    Plasmid

    #194503

    Purpose

    Expression foldon-GFP-PCP protein for imaging of nonrepetitive DNA

    Depositor

    Chunqing Song

    Article

    Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

    Insert

    foldon-GFP-PCP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α core promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW-HA-hRb-delta-CDK-puro

    Plasmid

    #212673

    Purpose

    Express tagged hRb delta-CDK phosphosite mutant

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    Rb (RB1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    All 15 CDK phospho-sites are mutated to alanines

    Promoter

    TRE

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLVX-Puro-TDP-43-K263E

    Plasmid

    #133758

    Purpose

    Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed lysine 263 to glutamate.

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCW-HA-hRb-WT-T373A-S608A-S612A-puro

    Plasmid

    #212674

    Purpose

    Express tagged hRb mutant

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    Rb (RB1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    T373A/S608A/S612A

    Promoter

    TRE

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGL4.26-Ccnd2PromoterRegion2(0.5kb)

    Plasmid

    #228059

    Purpose

    0.5 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

    Depositor

    Kenneth Chen

    Article

    Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

    Insert

    Ccnd2 promoter (Ccnd2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pGL4.26-Igf2Promoter

    Plasmid

    #228060

    Purpose

    0.9 kb from mouse Igf2 promoter in front of firefly luciferase reporter

    Depositor

    Kenneth Chen

    Article

    Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

    Insert

    Igf2 promoter (Igf2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PHB2 S39D-mCherry

    Plasmid

    #232787

    Purpose

    Encodes an mCherry-tagged Prohibitin2/PHB2 constitutively phosphorylated on Ser39

    Depositor

    Giulia Bertolin

    Article

    Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

    Insert

    PHB2 (PHB2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Serine 39 to Aspartate

    Promoter

    CMV

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3.1/MPL-HA

    Plasmid

    #204530

    Purpose

    Mammalian expression of human MPL-HA

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    MPL-HA (MPL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-Delta(Pro-MP)ADAM10-HA

    Plasmid

    #65107

    Purpose

    Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    Delta(Pro-MP)ADAM10 (ADAM10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted AA 19-455

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA3.1/CALR wt-FLAG

    Plasmid

    #204532

    Purpose

    Mammalian expression of human CALR wt-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR wt-FLAG (CALR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NowGFP /pQE-30

    Plasmid

    #74749

    Purpose

    NowGFP gene for bacterial expression

    Depositor

    Konstantin Lukyanov

    Article

    Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

    Insert

    NowGFP
    

    Use

    Tags

    HisTag (with a mutation but still working)

    Expression

    Bacterial

    Mutation

    WasCFP with substitutions E6K, L42M, T43S, V68M, …

    Promoter

    T5

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. pBABE HA Chk2 WT

    Plasmid

    #41901

    Depositor

    Stephen Elledge

    Article

    Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

    Insert

    CHK2 (CHEK2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge