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179,521 results

Showing: 6841 - 6880 of 179521 results

  3. SunTagng22aa

    Plasmid
    #106438
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Steven Jacobsen
    Publication
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106438-plasmid-map-sequence-id-309504

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  9. pET-BiFC

    Plasmid
    #87856
    Purpose
    pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.
    Depositor
    Dan Mulvihill
    Publication
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    Encodes for MCS & C-term half of mVenus
    Encodes for MCS & N-term half of mVenus
    Tags
    BiFC C-terminal fragment
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87856-plasmid-map-sequence-id-175019

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  16. CRY2(L348F)-CreN

    Plasmid
    #75368
    Purpose
    Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase
    Depositor
    Chandra Tucker
    Publication
    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRY2 (CRY2 Mustard Weed)
    Tags
    Cre recombinase, residues 19-104
    Expression
    Mammalian
    Mutation
    L348F
    Promoter
    CMV
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75368-plasmid-map-sequence-id-142723

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  21. MIP 339 CoMiP coLin28 2A Nanog IRES eGFP

    Plasmid
    #63729
    Purpose
    To express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
    Depositor
    Mark Kay, Joseph Wu
    Publication
    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    Tags
    T2A
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    SFFV
    Available since
    June 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    63729-plasmid-map-sequence-id-429278

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  24. pX330A_D10A-1x2

    Plasmid
    #58772
    Purpose
    Expresses Cas9 nickase and gRNA
    Depositor
    Takashi Yamamoto
    Publication
    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    CBh
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58772-plasmid-map-sequence-id-91082

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  32. 3xFLAG-dCas9/pMXs-neo

    Plasmid
    #51260
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51260-plasmid-map-sequence-id-77871

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  33. pcDNA3.2-Cx43-HA

    Plasmid
    #49851
    Purpose
    Encodes human connexin 43 with a C-terminal HA epitope fusion tag
    Depositor
    Robin Shaw
    Publication
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Connexin 43 (GJA1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Contains several wobble mutations to confer resis…
    Promoter
    CMV
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49851-plasmid-map-sequence-id-338136

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  35. pJBEI-6410

    Plasmid
    #47049
    Purpose
    BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Publication
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Expression
    Bacterial
    Mutation
    R364S mutation in MK
    Promoter
    PlacUV5
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47049-plasmid-map-sequence-id-496830

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Showing: 6841 - 6880 of 179521 results