164,577 results

1. pAAV-CMV-N-HA-Pcdh15_mini_V4

   Plasmid

   #199185

   Purpose

   An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

   Depositor

   David Corey

   Article

   Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

   Insert

   HA-mmPcdh15_mini_V4-CD2
   

   Use

   AAV

   Tags

   Human influenza hemagglutinin (HA)

   Expression

   Mutation

   Promoter

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-BrainbowTEC

   Plasmid

   #82405

   Purpose

   Gateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Inserts

   tdTomatoMYC fusion
   EGFP
   E2CrimsonHA
   

   Use

   Tags

   HA and MYC

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a T7pCONS TIR-2 sfGFP

   Plasmid

   #154464

   Purpose

   pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRE-UBC-hCD24-T2A-GFP

   Plasmid

   #205456

   Purpose

   lentiviral plasmid for expression of human CD24

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD24 (CD24 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-SYNZIP18

   Plasmid

   #80672

   Purpose

   Encodes the synthetic coiled coil peptide SYNZIP18

   Depositor

   Amy Keating

   Article

   Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

   Insert

   SYNZIP18
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-PGK-RAP1A-WT-puro

   Plasmid

   #156166

   Purpose

   Lentiviral vector for expression of RAP1A-WT, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1A (RAP1A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   wild type form

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX_314-HRI-V5

   Plasmid

   #202434

   Purpose

   Expression of HRI

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60229

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbS8c_ddCpf1_final

   Plasmid

   #212040

   Purpose

   Bacterial expression of the CRISPRi system for genome editing in E. coli

   Depositor

   Quanjiang Ji

   Article

   Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

   Insert

   ddCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   L-arabinose-inducible promoter PBAD

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PRDX1A-c001

    Plasmid

    #175473

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    PRDX1 (PRDX1 Human)
    

    Use

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-mDlx-GFP-Fishell-1

    Plasmid

    #83900

    Purpose

    GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    mDlx

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCKO2

    Plasmid

    #125518

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

    Depositor

    Jason Moffat

    Article

    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRISPR-SP-Cas9 reporter

    Plasmid

    #62733

    Purpose

    Mammalian CRISPR-SP-Cas9 reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    CRISPR-SP-Cas9 target seq + tdTomato (out of frame)
    

    Use

    CRISPR and Lentiviral

    Tags

    CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-Alpha

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. peGFP-N1-Cofilin

    Plasmid

    #186747

    Purpose

    Expresses wildtype GFP-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

    Plasmid

    #221233

    Purpose

    Express sgRNA targeting HEK3 loci with nCas9(D10A)

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(D10A)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221075

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-hG9a

    Plasmid

    #33025

    Depositor

    Martin Walsh

    Article

    Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

    Insert

    hG9a (EHMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. mATF4

    Plasmid

    #24874

    Depositor

    Fumihiko Urano

    Article

    Ghosh et al PLoS One. 2010 . 5(3):e9575.

    Insert

    mouse ATF4 (Atf4 Mouse)
    

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 17, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pSIBR004

    Plasmid

    #177667

    Purpose

    SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Flag-HA-USP5

    Plasmid

    #22590

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP5 (USP5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EB3-mNeonGreen

    Plasmid

    #98881

    Purpose

    In vivo visualization of microtuble plus ends (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    EB3
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAB076i3

    Plasmid

    #79198

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pE6n

    Plasmid

    #17460

    Purpose

    modified pENTR vector for N-terminal EYFP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    EYFP

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDEST17-UbE2G2

    Plasmid

    #15791

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2G2 (UBE2G2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pU6c:sgRNA#4

    Plasmid

    #64248

    Purpose

    expresses sgRNA under U6c promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. A*11:01:01:01

    Plasmid

    #203276

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*11:01:01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHT-6xR.ads-PxylA-DI

    Plasmid

    #47386

    Purpose

    Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Inserts

    ads
    dxs
    idi
    

    Use

    Tags

    6xR and His

    Expression

    Bacterial

    Mutation

    Promoter

    Pgrac and PxylA

    Available Since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

    Plasmid

    #101060

    Purpose

    AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mutation

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. K-SPOTIT1.0

    Plasmid

    #171215

    Purpose

    Expresses a kappa-opioid receptor-based circular permuted green fluorescent protein sensor

    Depositor

    Wenjing Wang

    Article

    Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

    Insert

    K-SPOTIT1.0
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMVHA hEZH2

    Plasmid

    #24230

    Purpose

    Mammalian expression of human EZH2 with HA tag

    Depositor

    Kristian Helin

    Article

    Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET15b His-p97

    Plasmid

    #169020

    Purpose

    Expresses His-p97 in bacteria

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-C-terminal GST

    Plasmid

    #128025

    Purpose

    Mammalian expression vector with C terminal GST tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pENN.AAV.tMCK.PI.eGFP.WPRE.bGH

    Plasmid

    #105556

    Purpose

    AAV expression of EGFP from tMCK promoter

    Depositor

    James M. Wilson

    Has Service

    AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tMCK

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAV.CAG.GLPLight1

    Plasmid

    #187467

    Purpose

    Expresses GLPLight1 in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

    Insert

    GLPLight1
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNAintron-EBOV-GP

    Plasmid

    #215692

    Purpose

    Produces the Ebola virus glycoprotein

    Depositor

    Melinda Brindley

    Article

    Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

    Insert

    EBOV-GP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

    Plasmid

    #132398

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Nb-gp41-Halo (MoonTag-Nb-Halo)

    Plasmid

    #128603

    Purpose

    Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    Nanobody_gp41
    

    Use

    Lentiviral

    Tags

    GB1 and Halo

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCS2+mNeonGreen-C Cloning Vector

    Plasmid

    #128144

    Purpose

    To express a protein of interest fused to the C-terminus of mNeonGreen

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    mNeonGreen fusion

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Stx5L-mCherry

    Plasmid

    #209844

    Purpose

    Expresses Stx5L-mCherry in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    STX5 (STX5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Wildtype Stx5 (with 55M)

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge