Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 16,952 results for: She

Showing: 8551 - 8600 of 16952 results

  1. PE-SUMO CRYBB1

    Plasmid
    #217675
    Purpose
    For expression of SUMO tagged human beta B1 crystallin
    Depositor
    Larry David
    Insert
    CRYBB1 (CRYBB1 Human)
    Use
    Tags
    6XHis and Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217675-plasmid-map-sequence-id-434867

    Enlarge

  2. PE-SUMO CRYAB

    Plasmid
    #217670
    Purpose
    For expression of SUMO tagged human alpha B crystallin
    Depositor
    Larry David
    Insert
    CRYAB (CRYAB Human)
    Use
    Tags
    6XHis and Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217670-plasmid-map-sequence-id-434869

    Enlarge

  4. PTPN11 in pENTR

    Plasmid
    #216604
    Purpose
    gateway cloning donor vector containing PTPN11
    Depositor
    Douglas Allan, Christopher Loewen
    Insert
    PTPN11 (PTPN11 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216604-plasmid-map-sequence-id-427162

    Enlarge

  5. pAAV-TREtight-mTagBFP2-N2cG

    Plasmid
    #192838
    Purpose
    plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA
    Depositor
    Ian Wickersham
    Insert
    mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TREtight
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    192838-plasmid-map-sequence-id-378336

    Enlarge

  8. Hs.KRAS4b G12V

    Plasmid
    #83132
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12V mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12V
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83132-plasmid-map-sequence-id-158095

    Enlarge

  9. p5'Entry-lck-5.5kb

    Plasmid
    #58890
    Purpose
    provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes
    Depositor
    Jeffrey Yoder
    Insert
    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
    Use
    Gateway 5' entry clone
    Tags
    Expression
    Mutation
    mutated single nucleotide (T-->C, 13th nucleot…
    Promoter
    lymphocyte-specific protein tyrosine kinase (lck)…
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58890-plasmid-map-sequence-id-91281

    Enlarge

  11. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid
    #218710
    Purpose
    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218710-plasmid-map-sequence-id-433357

    Enlarge

  12. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid
    #159912
    Purpose
    Mutagenesis of Kcnd3
    Depositor
    Larry Zweifel
    Insert
    Kcnd3 (Kcnd3 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159912-plasmid-map-sequence-id-317319

    Enlarge

  13. pTRE-T2-IRES-His6-Ubiquitin

    Plasmid
    #107107
    Purpose
    Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.
    Depositor
    Michael E. Wright
    Insert
    human ubiquitin UBB (UBB Human)
    Use
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Tet
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    107107-plasmid-map-sequence-id-218027

    Enlarge

  16. psd44-G13QL

    Plasmid
    #46828
    Purpose
    expression of mutant G protein alpha 13 in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    GNA13 (GNA13 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Q226L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46828-plasmid-map-sequence-id-137240

    Enlarge

  17. pAAV-UbC-eGFP-F

    Plasmid
    #71545
    Purpose
    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.
    Depositor
    Pantelis Tsoulfas
    Insert
    eGFP-F
    Use
    AAV
    Tags
    contains 20- amino-acid farnesylation signal from…
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    71545-plasmid-map-sequence-id-132622

    Enlarge

  18. pAG426-GAL-Alpha-synuclein-H50Q

    Plasmid
    #181724
    Purpose
    Galactose inducible expression of Alpha-synuclein-H50Q
    Depositor
    Alejandro Chavez
    Insert
    Alpha-synuclein (SNCA Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H50Q
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    181724-plasmid-map-sequence-id-431239

    Enlarge

  25. CD44Z-c011

    Plasmid
    #175455
    Purpose
    Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Use
    Tags
    His6
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175455-plasmid-map-sequence-id-353216

    Enlarge

  27. pScDD2_ERdd

    Plasmid
    #109047
    Purpose
    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).
    Depositor
    Thomas Wandless
    Insert
    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    Use
    Tags
    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…
    Expression
    Yeast
    Mutation
    3 mutations : E380G, R434G, N532S
    Promoter
    SOD1
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    109047-plasmid-map-sequence-id-209173

    Enlarge

  28. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid
    #105715
    Purpose
    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.
    Depositor
    Rylan Larsen
    Has Service
    AAV5
    Insert
    synaptophysin-GCaMP6s
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105715-plasmid-map-sequence-id-207274

    Enlarge

  32. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV and T7
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

    Enlarge

  33. pLKO.1-blast shKGA

    Plasmid
    #110420
    Purpose
    shKGA, silence glutaminase KGA isoform, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA Pol III)
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110420-plasmid-map-sequence-id-213695

    Enlarge

  35. Vector 1174D

    Plasmid
    #200012
    Purpose
    Fluorescent based sex separator in Aedes aegypti (SEPARATOR)
    Depositor
    Omar Akbari
    Article
    Weng et al bioRxiv. 2023 Jun 16:2023.06.16.545348.
    Insert
    Engineered dobulesex splicing module
    Use
    Synthetic Biology
    Tags
    Expression
    Insect
    Mutation
    Engineered dobulesex splicing module
    Promoter
    Hr5IE1
    Available since
    June 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200012-plasmid-map-sequence-id-395199

    Enlarge

  38. pBabe-Kras G12V-T35S

    Plasmid
    #58900
    Purpose
    Expresses human Kras with G12V and T35S mutations
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G12V and T35S mutations (effector binding mutant)
    Promoter
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  39. Hs.KRAS4b S17N

    Plasmid
    #83156
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    S17N
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83156-plasmid-map-sequence-id-158119

    Enlarge

  40. Hs.KRAS4b G12R

    Plasmid
    #83143
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12R
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83143-plasmid-map-sequence-id-158106

    Enlarge

  41. p-dCas9-p300-Hygro

    Plasmid
    #104411
    Purpose
    transient expression of dCas9-p300 fusion protein
    Depositor
    Stephan Beck
    Insert
    p300 (EP300 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104411-plasmid-map-sequence-id-204503

    Enlarge

  42. DOT1L

    Plasmid
    #36196
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (GeneScript Synthesized) (DOT1L Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-420
    Promoter
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  43. DOT1L

    Plasmid
    #40736
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (DOT1L Human)
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  48. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Mutation
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47912-plasmid-map-sequence-id-68534

    Enlarge

  50. pAG426-GAL-Alpha-synuclein-A53T

    Plasmid
    #181722
    Purpose
    Galactose inducible expression of Alpha-synuclein-A53T
    Depositor
    Alejandro Chavez
    Insert
    Alpha-synuclein (SNCA Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    A53T
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    181722-plasmid-map-sequence-id-439443

    Enlarge

Showing: 8551 - 8600 of 16952 results