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We narrowed to 8,624 results for: FIE

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Showing: 6881 - 6920 of 8624 results

  6. pFTK087

    Plasmid
    #171359
    Purpose
    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    P-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    171359-plasmid-map-sequence-id-351785

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  8. pPEE17

    Plasmid
    #128361
    Purpose
    Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Insert
    Acetamidase (amdS)
    Use
    Unspecified
    Promoter
    TEF1
    Available Since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    128361-plasmid-map-sequence-id-267942

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  13. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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  16. pFBCPDL6

    Plasmid
    #67285
    Purpose
    CPD photolyase from Drosophila with Flag tag in pFastbac vector. Intermediate in insect cell expression
    Depositor
    Aziz Sancar
    Article
    Selby et al Biochemistry. 2012 Jan 10;51(1):167-71.
    Insert
    CPD photolyase (phr Fly)
    Use
    Bacterial vector, insert not expressed
    Tags
    Flag
    Mutation
    V275E*
    Available Since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  24. Nup54 no FG-mEGFP494(0)

    Plasmid
    #164269
    Purpose
    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFP
    Depositor
    Sanford Simon
    Article
    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1
    Insert
    Nup54 (NUP54 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    deleted the FG region of Nup54
    Promoter
    CMV
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164269-plasmid-map-sequence-id-325395

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  27. pFTK093

    Plasmid
    #171365
    Purpose
    Vector part (LVL1) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    171365-plasmid-map-sequence-id-352204

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  31. pDEST(frt)(ATG_V5-APEX2_noSTOP)

    Plasmid
    #168207
    Purpose
    Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection
    Depositor
    Markus Hafner
    Article
    Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.
    Type
    Empty backbone
    Use
    Unspecified; Pdest gateway
    Tags
    V5 APEX2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168207-plasmid-map-sequence-id-331104

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Showing: 6881 - 6920 of 8624 results