163,606 results

1. plenti-ITGB6-HA

   Plasmid

   #235242

   Purpose

   Lentiviral vector to generate HA-tagged human ITGB6 stable expressing cell line

   Depositor

   Jan Carette

   Article

   Qiao et al Nat Commun. 2024 Apr 24;15(1):3469. doi:

   Insert

   ITGB6 (ITGB6 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1 F-tractin-EGFP

   Plasmid

   #58473

   Purpose

   Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   F-tractin (Itpka Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGCDNsam-Foxa1-IRES-GFP

   Plasmid

   #33007

   Depositor

   Atsushi Suzuki

   Article

   Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

   Insert

   forkhead box A1 (Foxa1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-UbC-rtTA-Ngn2:2A:EGFP

   Plasmid

   #127288

   Purpose

   all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

   Depositor

   Fred Gage

   Article

   Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

   Insert

   Ngn2:2A:Ascl1 (NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Ngn2 and EGFP linked via 2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiTet-OTX2-P2A-mCherry BlastR

   Plasmid

   #186741

   Purpose

   Dox inducible OTX2-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   OTX2 (OTX2 Human)
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. gTBEv5

   Plasmid

   #220621

   Purpose

   vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

   Promoter

   hU6, EF1a

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-FOXA2

   Plasmid

   #153110

   Purpose

   Expression of Flag tagged human FOXA2 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXA2 (FOXA2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CibN

   Plasmid

   #209671

   Purpose

   Expresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cells

   Depositor

   Sanjeevi Sivasankar

   Article

   Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

   Insert

   CIB1 (CIB1 Mustard Weed)
   

   Use

   Tags

   Split-TurboID-N and pmEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQC NLS mCherry IX

   Plasmid

   #37354

   Depositor

   Connie Cepko

   Article

   Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

   Insert

   NLS-mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. ilux pGEX(-)

    Plasmid

    #107879

    Purpose

    Bacterial expression of ilux for bioluminescence imaging

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

    Insert

    ilux
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS_cAMPFIRE-M

    Plasmid

    #182284

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-M (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX333-T2A-EGFP

    Plasmid

    #197417

    Purpose

    SpCas9-T2A-EGFP with dual sgRNA cloning backbones

    Depositor

    Yeh-Hsing Lao

    Article

    Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh; U6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHBS838 TDP43 RRM-GFP

    Plasmid

    #98249

    Purpose

    TDP43 RRM-GFP reporter construct

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Cell Rep. 2016 Aug 2;16(5):1228-36. doi:

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    Internal GFP to replace RRM domains

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcFLAG-FYN-WT

    Plasmid

    #74509

    Purpose

    Overexpression of FYN in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Chougule et al Oncotarget. 2016 Feb 2. doi: 10.18632/on

    Insert

    FYN (FYN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-Solo-mStayGold-VIM

    Plasmid

    #227302

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the VIM locus. For intermediate filament visualization. To be co-transfected with sgRNA plasmid px330-PITCh-VIM (Addgene #227301)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    VIM Homology Arms flanking a mStayGold Tag (VIM Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-PITCh-VIM

    Plasmid

    #227301

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of VIM (VIM Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-GFP-NMHC II-A

    Plasmid

    #11347

    Depositor

    Robert Adelstein

    Article

    Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

    Insert

    NMHC II-A (MYH9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid

    #118156

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSI-Check2-hRluc-NFkB-firefly

    Plasmid

    #106979

    Purpose

    Luciferase reporter with internal control for NFkB signal.

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    NFKB recognition sites (NFKB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-Rab4B

    Plasmid

    #61801

    Purpose

    Human Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab4B (RAB4B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. IKK-2 S177E S181E

    Plasmid

    #11105

    Purpose

    Mammalian expression of constitutively active human IKK-2

    Depositor

    Anjana Rao

    Article

    Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

    Insert

    IKK-2 (IKBKB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S177E and S181E, constitutively active

    Promoter

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLVX-Tet3G blasticidin

    Plasmid

    #128061

    Purpose

    Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HMGCS2

    Plasmid

    #38984

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HMGCS2 (HMGCS2 Human)
    

    Use

    Tags

    His6-Thioredoxin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

    Plasmid

    #111568

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2w/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Has Service

    AAV5

    Insert

    Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-N1-TFE3

    Plasmid

    #38120

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFE3 (TFE3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid

    #140980

    Purpose

    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasS-RLuc8
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCSN075

    Plasmid

    #166732

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS-Mxi1
    

    Use

    CRISPR

    Tags

    Mxi1 and SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pDR-GW AT1.03YEMK

    Plasmid

    #28004

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    AT1.03YEMK
    

    Use

    Tags

    mVenus and mseCFP

    Expression

    Yeast

    Mutation

    M60Y, G131E, K132M, 133K

    Promoter

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Mammalian expression: Z-lock cofilin dark state mutant

    Plasmid

    #141136

    Purpose

    a control for optogenetic cofilin analog

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmVenus(L68V)-mTurquoise2

    Plasmid

    #60493

    Purpose

    Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    SYFP2-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge