We narrowed to 156,021 results for: ins
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Plasmid#127237PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterhSyn1Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM255 - sgRNA mosTI Pmlc-2_GFP
Plasmid#159835PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertsgRNA mosTI Pmlc-2_GFP
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS423-GPD-wrmScarlet1-10-CYC1 terminator
Plasmid#158584PurposeExpresses wrmScarlet1-10 in S. cerevisiaeDepositorInsertwrmScarlet1-10
ExpressionYeastPromoterGPDAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
CapChR2_pmScarlet-N1
Plasmid#188032PurposeExpresses CapChR2-mScarlet in mammalian cellsDepositorInsertCapChR2-mScarlet
TagsmScarletExpressionMammalianMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPrev
Plasmid#158060PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyExpressionBacterialPromoteracpPAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L MBP-SspB Micro
Plasmid#60410PurposeE. coli expression of SspB MicroDepositorInsertSspB micro
TagsHis-MBP-TEVExpressionBacterialMutationR73Q, Y11K and A15EAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro human IRS-1
Plasmid#11025DepositorAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDB180
Plasmid#80677Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged proteinDepositorTypeEmpty backboneTagsOsmY-10His-Smt3ExpressionBacterialPromoterT7Available SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
HcKCR1_mCherry_pcDNA3.1
Plasmid#177336PurposeExpresses KCR1 in mammalian cellsDepositorInsertHcKCR1
TagsmCherryExpressionMammalianMutationmammalian codon-adaptedAvailable SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hTGFa
Plasmid#199236PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertTransforming growth factor alpha, partial (TGFA Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
VPS13A^Halo
Plasmid#118759PurposeVPS13A internally tagged with Halo at residue 1372.DepositorAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM319 - array co-injection fragment mosTI unc-119
Plasmid#159823PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertarray co-injection fragment mosTI unc-119
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L iLID (C530M)
Plasmid#60408PurposeE. coli expression of iLID (C530M)DepositorInsertiLID C530M
TagsHisExpressionBacterialMutationC530MAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX PLCg1(N)-SH2
Plasmid#46470DepositorInsertPLCg1(N)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 539-664PromoterTacAvailable SinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME-ReaChR-citrine
Plasmid#169045PurposeMiddle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insertDepositorInsertReaChR-citrine
UseGateway entry cloning vectorTagscitrineAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mNeptune2-N
Plasmid#41645PurposeExpresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)DepositorInsertmNeptune2-Dronpa145N
ExpressionMammalianMutationK145N on Dronpa (allows control of photochromic F…PromoterCMVAvailable SinceDec. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G
Plasmid#167942PurposeInducible NKX2-1 overexpressionDepositorAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChRmine-mRuby2-ST
Plasmid#227777PurposeCation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitationDepositorInsertChRmine-mRuby2-ST
ExpressionMammalianAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECIA2
Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: tgRFPt-SSPB R73Q
Plasmid#60416PurposeMammalian Expression of tgRFPt-MicroDepositorInserttgRFPt-SSPB R73Q
UseCre/Lox and LentiviralTagsTagRFPtExpressionMammalianMutationR73Q, Y11K and A15EPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDV24_AAV2_DART-VADAR_iRFP720-sensor
Plasmid#200411PurposeAAV vector encoding a DART-VADAR sensor for iRFP720 mRNADepositorInsertDART VADAR sensor
UseAAV and Synthetic BiologyTagsMCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreenExpressionMammalianPromoterCMVAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS human IRS-1
Plasmid#11359DepositorInsertIRS-1 (IRS1 Human)
ExpressionBacterialAvailable SinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTK331
Plasmid#59572PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP(16) 2xK-turn Kozak EGFP attB2 AscI
Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro GFP shRNA
Plasmid#10675PurposeNegative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.DepositorInsertGFP shRNA
UseRNAi and RetroviralExpressionMammalianAvailable SinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
SQT1665
Plasmid#78744PurposeHuman expression plasmid for LbCpf1-NLS-3xHADepositorInsertmammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCAGAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChroMD-mRuby2-ST
Plasmid#171158PurposeCation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroMD
ExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag-P2A-tagBFP
Plasmid#174141PurposeExpresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.DepositorInsertdCas9, 10xSunTag, tagBFP
TagsHA and tagBFPExpressionMammalianPromoterSV40Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChroME2f-mRuby2-ST
Plasmid#170160PurposeCation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroME2f-mRuby2-ST
ExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only