We narrowed to 17,014 results for: por

1. mNeonGreen-P2A-3xFLAG-msEEA1 HDR template

   Plasmid

   #214990

   Purpose

   Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   EEA1 (Eea1 Mouse)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAS12124_PEAR-GFP-preedited

   Plasmid

   #177179

   Purpose

   PEAR-GFP control plasmid with split EGFP and intact 5' splice site

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Mut2-RS

   Plasmid

   #182882

   Purpose

   A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

   Depositor

   Miriam Amiram

   Article

   Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

   Insert

   Mut2-RS
   

   Expression

   Bacterial

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCCl-MCS_miniCMV_TagBFP

   Plasmid

   #134986

   Purpose

   The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

   Depositor

   Claudio Mussolino

   Article

   Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

   Insert

   TagBFP
   

   Use

   Lentiviral

   Promoter

   minCMV

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPBCAG-rtTAM2-IH

   Plasmid

   #140827

   Purpose

   Expresses rtTAM2-IRES-HygR in mammalian cells

   Depositor

   Hideyuki Okano

   Article

   Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

   Inserts

   rtTAM2
   HygR
   

   Expression

   Mammalian

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPIDCB2

   Plasmid

   #169900

   Purpose

   Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.

   Depositor

   Richard Schneider

   Article

   Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

   Type

   Empty backbone

   Use

   Avian expression

   Expression

   Mammalian

   Promoter

   Dox inducible

   Available Since

   Dec. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLD-puro-CcVA

   Plasmid

   #24588

   Depositor

   Jason Moffat

   Article

   Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

   Insert

   Gateway(TM) cassette
   

   Use

   Lentiviral

   Tags

   VA

   Expression

   Mammalian

   Available Since

   Aug. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-2H12

   Plasmid

   #221186

   Purpose

   His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-LBR-mRFP1-polyA

   Plasmid

   #59749

   Purpose

   Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   LBR-mRFP1
   

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   aa 1-249 of LBR

   Promoter

   CMV and T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57.1-3xFLAG-NLS-GFP

    Plasmid

    #198238

    Purpose

    Lentivector with tet-inducible 3xFLAG-NLS-GFP

    Depositor

    Stephen Tapscott

    Article

    Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

    Insert

    3xFLAG-NLS-GFP
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. eGFP L138 gRNA

    Plasmid

    #119133

    Purpose

    gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    eGFP L138 gRNA
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    U6

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast

    Plasmid

    #205549

    Purpose

    Express EPN24-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWXLd-RIEP-GpNLuc

    Plasmid

    #135935

    Purpose

    Expresses the GpNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJWV25

    Plasmid

    #61045

    Purpose

    To construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaA

    Depositor

    Jan-Willem Veening

    Article

    Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GFP+

    Expression

    Bacterial

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPIDNB

    Plasmid

    #169901

    Purpose

    Empty dox inducible vector with piggyBac, insulators, and a DTS

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Aivian expression

    Expression

    Mammalian

    Promoter

    Dox inducible

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2H12

    Plasmid

    #221142

    Purpose

    cdGreen expressed from an aTc-inducible promoter (Internal Lab ID: UJ11204)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSplit-CO-hDib1

    Plasmid

    #58788

    Purpose

    Express LucC-hDib1 fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN_TT 3xFLAG-XRCC6

    Plasmid

    #192302

    Purpose

    Gateway entry vector for an inducible 3xFLAG-XRCC6

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    XRCC6 (XRCC6 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5_tAKARα

    Plasmid

    #119913

    Purpose

    Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARα
    

    Expression

    Mammalian

    Available Since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPD1028 ZF43x6-Compact(SV40Min)_EYFP

    Plasmid

    #138912

    Purpose

    EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a SV40 minimal promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Compact(SV40min)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    ZF43x6-Compact(SV40min)

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pRN3P_T3_ABEmax_IVT

    Plasmid

    #171761

    Purpose

    Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

    Insert

    ABEmax
    

    Use

    Vector for in-vitro transcription

    Promoter

    T3 promoter

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDctp-mSox10-Mp1

    Plasmid

    #13513

    Depositor

    William Pavan

    Article

    Hakami et al Mech Dev. 2006 Feb . 123(2):124-34.

    Insert

    Sox10 (Sox10 Mouse)
    

    Tags

    Dct promoter and Mp1-polyA

    Expression

    Mammalian

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCS2-QFGal4-SV40pA

    Plasmid

    #155126

    Purpose

    In vitro transcription of QFGal4 mRNA

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QFGal4
    

    Use

    Expression in zebrafish

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. Orai1-myc E106Q MO70

    Plasmid

    #22754

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 E106Q (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    E106Q

    Available Since

    Jan. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCLC3sHA

    Plasmid

    #52424

    Purpose

    mammalian expression of HA tagged rat CLC3 short transcript

    Depositor

    Steven Weinman

    Article

    Wang et al Gastroenterology. 2004 Apr;126(4):1157-6

    Insert

    CLC3 (Clcn3 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. ptRNA100

    Plasmid

    #154133

    Purpose

    Expresses a set of tRNAs in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

    Insert

    tRNAs operon under control of Ptac promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pTUP1

    Plasmid

    #104341

    Purpose

    Express TUP1

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

    Insert

    TUP1 (TUP1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA3 tdAPX W41F

    Plasmid

    #40863

    Purpose

    Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    tandem dimer pea APX W41F
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    W41F mutation

    Promoter

    CMV

    Available Since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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29. CMV-ShadowY-kCAAX

    Plasmid

    #115564

    Purpose

    dark ITRACK acceptor construct for dual color imaging

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    ShadowY with KRas membrane targeting domain
    

    Tags

    ShadowY

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pX330-Flag-wtSpCas9-H840A

    Plasmid

    #80453

    Purpose

    SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    SpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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