We narrowed to 13,571 results for: GAN

1. pET21-pelB-LaG-26

   Plasmid

   #172751

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-26
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. c3GIC9n

   Plasmid

   #62192

   Purpose

   col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

   Depositor

   Lukas Dow

   Article

   Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

   Insert

   humanized S. Pyogenes Cas9 (D10A)
   

   Use

   CRISPR

   Tags

   HA

   Mutation

   D10A substitution (Cas9n)

   Promoter

   TRE3G

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS_FLPER(T2)

   Plasmid

   #31303

   Depositor

   Gerhart Ryffel

   Article

   Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

   Insert

   FLP-ER ligand binding domain (ESR1 Human, Budding Yeast)
   

   Expression

   Mammalian

   Mutation

   full length FLP linked to ligand binding domain o…

   Available Since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-ExRai-AKAR-NES

   Plasmid

   #118407

   Purpose

   Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR-NES
   

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. iMAP-61

   Plasmid

   #187460

   Purpose

   gRNA array of iMAP-61

   Depositor

   Tian Chi

   Article

   Liu et al Cell. 2022 Aug 4;185(16):3008-3024.e16.

   Insert

   sgRNA array
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   modified U6

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. ZJOM156

   Plasmid

   #133636

   Purpose

   Integration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   SEC7-2GFP (SEC7 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP

   Plasmid

   #32479

   Purpose

   Cre-dependent expression of PSAM-GlyR (L141F) neuronal inhibitor, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAML141F-GlyR-IRES-GFP
   

   Use

   AAV and Cre/Lox; Adeno-associated virus, flex swi…

   Tags

   IRES-EGFP

   Promoter

   Synapsin

   Available Since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSO221 Phis-72 BirA GFP

   Plasmid

   #45100

   Depositor

   Steven Henikoff

   Article

   Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

   Inserts

   BirA::GFP
   unc-119 (unc-119 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   his-72 and unc-119

   Available Since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL2622 (pCas7_Vch)

   Plasmid

   #200894

   Purpose

   Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCas7
   

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PA-mCherry-miniSOG-Paxillin-22

    Plasmid

    #57796

    Purpose

    Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Paxillin
    

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pAM-AAV-CAG-EGFP

    Plasmid

    #153186

    Purpose

    CAG promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SG3ΔENV CA Q63A,Q67A

    Plasmid

    #145784

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q63A,Q67A mutation in capsid (CA Q63A,Q67A).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Capsid
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    Changed Capsid residues Glutamines 63 and 67 to a…

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA

    Plasmid

    #220224

    Purpose

    AAV-STARR-seq validation control vector with Venus

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)

    Plasmid

    #193853

    Purpose

    Promoterless insertion vector for split HygR/mScarlet-I landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' rps-0::HygR + 5' mScarlet-I
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Promoter

    rps-0p

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTK0_051

    Plasmid

    #123948

    Purpose

    Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hAAVS1 landing pad destination vector
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. DNA-CARtcr-IRESpuro

    Plasmid

    #89346

    Purpose

    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
    

    Use

    Lentiviral

    Tags

    SNAPf tag

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-APEX2-P2A-mKate2

    Plasmid

    #67671

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-P2A-mKate2
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. PA-mCherry-miniSOG-Lysosomes-20

    Plasmid

    #57793

    Purpose

    Localization: Lysosome Membrane, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    LAMP1
    

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    D50E in LAMP1

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSF1C-A-RFP

    Plasmid

    #172431

    Purpose

    StrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

    Depositor

    Simon Moore, Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21-pelB-LaG-11

    Plasmid

    #172762

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-11
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ZJOM25

    Plasmid

    #133672

    Purpose

    Integration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-MSP1-2GFP (MSP1 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine

    Plasmid

    #104122

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    spine-jRGECO1a-P2A-QuasAr-dark Citrine
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJM160

    Plasmid

    #134169

    Purpose

    Bacterial expression of RB3 stathmin-like domain (SLD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    RB3-SLD (STMN4 Human)
    

    Expression

    Bacterial

    Mutation

    residues 49-189

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MTK0_003

    Plasmid

    #123925

    Purpose

    Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Cas9-sgRNA destination
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUBC_HA_PP7-mCherry

    Plasmid

    #107605

    Purpose

    Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    PP7-mCherry
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Mutation

    PP7: [delta]67_74 (non-aggregating)

    Promoter

    hUBC

    Available Since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21-pelB-LaG-21

    Plasmid

    #172749

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-21
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET21-pelB-LaG-37

    Plasmid

    #172756

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-37
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pENTR_hEMSY

    Plasmid

    #125156

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    EMSY (EMSY Human)
    

    Use

    Gateway entry vector

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHES941

    Plasmid

    #87942

    Purpose

    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    GAL4 DBD - hPR LBD - MSN2 AD (PGR Human, Synthetic, Budding Yeast)
    

    Expression

    Yeast

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TetO-FUW-mir124a

    Plasmid

    #61539

    Purpose

    Expresses mir124a in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    mir124a (MIR124-1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

31. DNA-CARzeta-IRESpuro

    Plasmid

    #89345

    Purpose

    2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    DNA-CARzeta
    

    Use

    Lentiviral

    Tags

    SNAPf tag

    Expression

    Mammalian

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTorPE-IP2.0

    Plasmid

    #109217

    Purpose

    Express IP2.0 in E. coli

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1(+)-Mito-CaNARi

    Plasmid

    #64851

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Tags

    Cerulean, N-terminal 30 amino acids from DAKAP1, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET21-pelB-LaM-1

    Plasmid

    #172767

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-1
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Orai1

    Plasmid

    #12199

    Depositor

    Anjana Rao

    Article

    Feske et al Nature. 2006 May 11. 441(7090):179-85.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

36. pKC158

    Plasmid

    #134242

    Purpose

    CENP-A Halo expression construct

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    CENP-A-Halo tagging construct (CENPA Human)
    

    Use

    Human targeting

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p6895 PHAGE-P CMVT N-HA-hE6AP I wt

    Plasmid

    #37601

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP I wt (UBE3A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV tet regulatable

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHH0103_PIKFYVE_DEP

    Plasmid

    #109789

    Purpose

    Protein expression and purification of PIKFYVE_DEP

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PIKFYVE_DEP (PIKFYVE Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLV-EF1a-V5-Smpd4

    Plasmid

    #120237

    Purpose

    Expresses V5-tagged Smpd4 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Smpd4 (Smpd4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET21-pelB-LaG-29

    Plasmid

    #172753

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-29
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge