162,221 results

1. pDONR223_FBXW7_WT

   Plasmid

   #81795

   Purpose

   Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   FBXW7 (FBXW7 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDUAL SRBI (GFP)

   Plasmid

   #86979

   Purpose

   Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

   Depositor

   Joe Grove

   Insert

   SCARB1 (SCARB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV/hPGK

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_ETS1_WT

   Plasmid

   #82118

   Purpose

   Gateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   ETS1 (ETS1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BFP-Sec61 beta

   Plasmid

   #49154

   Purpose

   expression of Sec61 beta fused to BFP, used as a general ER marker

   Depositor

   Gia Voeltz

   Article

   Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1481 pcDNA3 flag HA 14-3-3 zeta

   Plasmid

   #9002

   Depositor

   William Sellers

   Insert

   14-3-3 zeta (YWHAZ Bovine)
   

   Use

   Tags

   HA and flag

   Expression

   Mammalian

   Mutation

   T226A

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRwB

   Plasmid

   #48734

   Purpose

   dsRed plasmid

   Depositor

   John Schiller

   Article

   Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

   Insert

   dsRed-Express
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-ChRmine-mScarlet-WPRE

   Plasmid

   #130990

   Purpose

   AAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-HA-HA-human CMYC S62A

   Plasmid

   #203436

   Purpose

   Mammalian expression of MYC

   Depositor

   Yoshiaki Ito

   Article

   Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   2 x HA

   Expression

   Mammalian

   Mutation

   S62A

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHM840

   Plasmid

   #20701

   Depositor

   Thomas Stamminger

   Article

   Sorg et al Biotechniques. 1999 May . 26(5):858-62.

   Insert

   GFP-NLS-lacZ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHEN2-SG4 R105A

    Plasmid

    #196072

    Purpose

    Express mutant (R105A) SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4 R105A
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    R105A mutation in SG4

    Promoter

    T7

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. C098

    Plasmid

    #174345

    Purpose

    Template for making Nluc mRNA reporter with pA139.

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    NanoLuc luciferase
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGR595

    Plasmid

    #213785

    Purpose

    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

    Depositor

    Chang Liu

    Insert

    BadBoy2 TP-DNAP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNP1954

    Plasmid

    #222730

    Purpose

    pNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDAC439

    Plasmid

    #195237

    Purpose

    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-CMV-TetO2_mRuby2

    Plasmid

    #113894

    Purpose

    Mammalian (inducible) expression of cytosolic mRuby2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    monomeric Ruby2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

    Plasmid

    #203228

    Purpose

    Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

    Depositor

    Adam Cohen

    Insert

    Membrane-localized optopatch for neuronal dendrites
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-Akt1(WT)

    Plasmid

    #86637

    Purpose

    Expresses eGFP-tagged human Akt1(WT)

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. FUGW-d2GFP-200

    Plasmid

    #79602

    Purpose

    FUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sites

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    d2GFP + miR-200 family targets
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-Bxb1-His

    Plasmid

    #123132

    Purpose

    Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Bxb1 integrase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pATetO 6XHis T4 DNA Ligase

    Plasmid

    #206207

    Purpose

    Inducible expression of T4 DNA ligase

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    T4 DNA ligase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 1479 pcDNA3 flag HA 14-3-3 gamma

    Plasmid

    #9000

    Depositor

    William Sellers

    Insert

    14-3-3 gamma (Ywhag Rat)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

22. pHR_HNF1A-RE_tBFP_PGK_mCitrine

    Plasmid

    #188387

    Purpose

    Lentiviral vector - tBFP reporter for HNF1A-based SNIPRs with a constitutive mCitrine;

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    HNF1A-RE_tBFP_PGK_mCitrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Plasmid

    #154430

    Purpose

    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Depositor

    David Liu

    Article

    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

    Insert

    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    VRQR point mutations in SpCas9

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HP17_KO7

    Plasmid

    #120346

    Purpose

    Helper plasmid for production of phagemid ssDNA, based on M13KO7

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

    Insert

    Coding part of the M13KO7 helper phage genome
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native phage promoters

    Available since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti6.2-ccdB-Nanoluc

    Plasmid

    #87075

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pInducer10-mir-RUP-PheS

    Plasmid

    #44011

    Purpose

    Lentiviral shRNA vector (pGIPz based) for inducible knockdown

    Depositor

    Stephen Elledge

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE2

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PRS_8-eGFP

    Plasmid

    #192589

    Purpose

    AAV-vector for fluorescent labeling of noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRSX8

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHCMV_VSBV_G

    Plasmid

    #207312

    Purpose

    Mammalian expression of Variegated Squirrel Bornavirus G protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    VSBV_G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. eGFP-proα1(I)

    Plasmid

    #119843

    Purpose

    Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α1 chain (Col1a1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Col1a1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUBC-SV40NLS-Pfv-Sapphire-PURO

    Plasmid

    #203654

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GFP-HP1beta

    Plasmid

    #17651

    Depositor

    Tom Misteli

    Article

    Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

    Insert

    HP1 beta (CBX1 Human)
    

    Use

    Tags

    GFP and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET22b-mEB3-eGFP-6xHis

    Plasmid

    #130983

    Purpose

    Bacterial expression of mouse EB3-GFP-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB3-GFP-6xHis (Mapre3 Mouse)
    

    Use

    Tags

    eGFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SIRT2 Flag

    Plasmid

    #13813

    Purpose

    Mammalian expression of human SIRT2 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Transcript variant 2

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBS-ermGFP

    Plasmid

    #27172

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBA409

    Plasmid

    #85971

    Purpose

    EF1a-sfGFP-Ubc-Neo

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sfGFP
    Neo
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

    Plasmid

    #64216

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-EGFP-C-IFT88

    Plasmid

    #218733

    Purpose

    Expresses N-terminally EGFP-tagged IFT88 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

    Insert

    IFT88 (IFT88 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p70a-T7RNAP

    Plasmid

    #105683

    Purpose

    Expresses T7 polymerase in cell-free TXTL.

    Depositor

    Chase Beisel

    Article

    Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

    Insert

    T7 polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HA-Rab11-WT

    Plasmid

    #101047

    Purpose

    Expresses HA-tagged human Rab11-WT in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    RAB11 (RAB11A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJRK261

    Plasmid

    #173754

    Purpose

    CeLINC plasmid

    Depositor

    Mike Boxem

    Article

    Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

    Insert

    (empty)::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge