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Showing: 7201 - 7240 of 157732 results

  7. FURIN-bio-His

    Plasmid
    #51755
    Purpose
    Expresses full-length Furin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    FURIN (FURIN Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    51755-plasmid-map-sequence-id-79339

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  14. pDL025_HA-rb_IgG_HC_15F11

    Plasmid
    #193622
    Purpose
    HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.
    Depositor
    Jennifer DeLuca
    Article
    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:
    Insert
    Anti_HA_15F11 rabbit heavy chain
    Use
    Tags
    signal peptide
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193622-plasmid-map-sequence-id-446783

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  15. pDL026_HA-rb_IgG_LC_15F11

    Plasmid
    #193623
    Purpose
    HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.
    Depositor
    Jennifer DeLuca
    Article
    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:
    Insert
    Anti-HA_15F11 rabbit light chain
    Use
    Tags
    signal peptide
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193623-plasmid-map-sequence-id-380458

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  19. pcDNA3.1-MAP3K5 (WT)

    Plasmid
    #47104
    Purpose
    Expresses wild type human MAP3K5 in mammalian cells
    Depositor
    Yardena Samuels
    Insert
    MAP3K5 (MAP3K5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    NA
    Promoter
    CMV
    Available since
    Jan. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    47104-plasmid-map-sequence-id-214692

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  23. TFORF0509

    Plasmid
    #143036
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TSHZ2 (TSHZ2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    143036-plasmid-map-sequence-id-385778

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  25. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  26. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid
    #172603
    Purpose
    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    The multiple cloning site (MCS) has been edited/e…
    Promoter
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172603-plasmid-map-sequence-id-340216

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  34. pL97

    Plasmid
    #78576
    Purpose
    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter
    Depositor
    Xuming Mao
    Article
    Sun et al Annals of Microbiology Dec 2012, Volume
    Type
    Empty backbone
    Use
    Streptomyces expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ermEp* promoter from S. erythraea
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    78576-plasmid-map-sequence-id-421926

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  36. ampl43

    Plasmid
    #161830
    Purpose
    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.
    Depositor
    Saeed Tavazoie
    Article
    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    161830-plasmid-map-sequence-id-318315

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  38. FUGW-APOE-E2-T2A-mCherry

    Plasmid
    #190807
    Purpose
    Express APOE E2 allele from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E2
    Promoter
    Ubi C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190807-plasmid-map-sequence-id-372851

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  39. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71667-plasmid-map-sequence-id-243369

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  40. p75-RFP

    Plasmid
    #24092
    Depositor
    Moses Chao
    Insert
    p75NTR (NGFR Human)
    Use
    Tags
    DSRed
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    24092-plasmid-map-sequence-id-210141

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Showing: 7201 - 7240 of 157732 results