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IBMc097

Plasmid

#161945

Purpose

Contains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherry

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI

Expression

Bacterial

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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enAsABE8e-dimer

Plasmid

#138507

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐Afu‐rnhBΔPIP

Plasmid

#108696

Purpose

For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

rnhB

Tags

GST

Expression

Bacterial

Mutation

C-terminal truncation (7aa) removing PIP box

Promoter

tac

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐Afu‐rnhB D101N

Plasmid

#108695

Purpose

For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

rnhB

Tags

GST

Expression

Bacterial

Mutation

D101N catalytic site mutation

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-SLC30A10-N43H

Plasmid

#82344

Purpose

Expresses FLAG-tagged SLC30A10-N43H in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

N43H

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLL7.0: Venus-oLID-CAAX (from KRas4B)

Plasmid

#60412

Purpose

Mammalian Expression of Venus-oLID-CAAX

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-oLID-CAAX

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-SLC30A10-D248A

Plasmid

#82346

Purpose

Expresses FLAG-tagged SLC30A10-D248A in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D248A

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO MycLAP-STIL ∆CC

Plasmid

#80268

Purpose

mammalian expression plasmid for MycLAP-STIL coiled-coil deletion

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Tags

Myc-GFP

Expression

Mammalian

Mutation

missing conserved coiled-coil (amino acids 721-74…

Promoter

CMV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 E144K

Plasmid

#167193

Purpose

puromycin resistant lentiviral vector for expression of PAK5 E144K

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 E144K

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 M173I

Plasmid

#167192

Purpose

puromycin resistant lentiviral vector for expression of PAK5 M173I

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 M173I

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-fTCAP-GFP

Plasmid

#22916

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu titan cap promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pGex-3x Flag MKK3

Plasmid

#14814

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK3 (MAP2K3 Human)

Tags

Flag

Expression

Bacterial

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mm

Plasmid

#51059

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-16-Decoy.hPGK.GFP.WPRE

Plasmid

#46593

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-16 Decoy (Mir16-1 Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pCAGGS-PB1-MontiRed-LOV2dark

Plasmid

#178874

Purpose

Mammalian expression of PB1-MontiRed-LOV2dark

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-MontiRed-LOV2dark

Expression

Mammalian

Mutation

LOV2: C450A, L514K, G528A, L531E, N538E

Promoter

CAG

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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myc-hIFITM3-F63Q

Plasmid

#104363

Purpose

Expresses human IFITM3-F63Q with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

Insert

IFITM3 (IFITM3 Human)

Tags

myc

Expression

Mammalian

Mutation

mutated the following amino acid: F63Q

Promoter

CMV

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-Patronin CC+CKK

Plasmid

#59048

Purpose

for baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC 534–1457

Plasmid

#59050

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
MS2-adRNA (3'UAG)

Plasmid

#170128

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'UAG)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP86

Plasmid

#83543

Purpose

Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL4A promoter-yEGFP

Expression

Yeast

Promoter

RPL4A

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-AsCas12a_crRNA-site3 (RTW549)

Plasmid

#160138

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHDV

Plasmid

#51058

Purpose

Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pG.Lantern-SM50-GFP

Plasmid

#135593

Purpose

Expression of SM50 Promoter-GFP mRNA for microinjection to visualize SM50 promoter activity in S. purpuratus

Depositor

Koji Akasaka, Mamiko Yajima

Article

Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

Insert

SM50 promoter (SM50 S. purpuratus)

Use

In vitro transcription (mrna synthesis)

Tags

Green Lantern GFP

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pWP1_mFTCD

Plasmid

#102317

Purpose

expression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Ftcd (Ftcd Mouse)

Use

Lentiviral

Promoter

EF1alpha

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b+R3(R112C/T180C)

Plasmid

#108195

Purpose

Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA3(R112C/T180C) fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-cofilin1(S3A)-P2A-mTurquoise2

Plasmid

#163610

Purpose

Expresses constitutively active cofilin and mTurquoise2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

cofilin1 (Cfl1 Mouse)

Tags

mTurquoise2

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-ChroME(F243Y)-mRuby2-ST

Plasmid

#109127

Purpose

Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChroME(F243Y)-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTetR-BCD2-Ana GvpC

Plasmid

#153298

Purpose

Expresses wild-type gas vesicle protein C (GvpC) from Anabaena flos-aquae

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Gas vesicle protein C

Expression

Bacterial

Promoter

pTet

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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D92_Ld_360170

Plasmid

#135905

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pM117: VEGFA array presgRNA

Plasmid

#166869

Purpose

U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

Human VEGFA targeting array presgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pSFFV-Cx43

Plasmid

#69442

Purpose

mammalian expression of rat Cx43

Depositor

Richard Veenstra

Article

Veenstra et al Circ Res. 1995 Dec;77(6):1156-65.

Insert

Cx43 (Gja1 Rat)

Expression

Mammalian

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
8xCTS1_BpiI-cloning-site_SV40polyA

Plasmid

#124547

Purpose

Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-malG

Plasmid

#89951

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

malG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-NS1-HilA

Plasmid

#153327

Purpose

Triggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-NS1 Monobody

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM3-C105A

Plasmid

#58392

Purpose

Expressed murine IFITM3-C105A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 105 to Alanine

Promoter

CMV IE

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only
AV4-EF1-GFP

Plasmid

#124971

Purpose

Control for AV4-EF1-NMNAT2

Depositor

Kazuhiro Oka

Article

Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

Insert

GFP

Use

AAV

Promoter

EF1-alpha

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pPK1E-2+t

Plasmid

#48148

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP84

Plasmid

#83542

Purpose

Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSA1 promoter-yEGFP

Expression

Yeast

Promoter

SSA1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ABE8e(TadA-8e V82G)

Plasmid

#138494

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e V82G)-nSpCas9

Expression

Mammalian

Mutation

TadA-8e V82G

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-h-mmPtch1-B-HA

Plasmid

#120902

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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