162,160 results

1. pcDNA3/HF-CDC50A

   Plasmid

   #209223

   Purpose

   Mammalian expression of CDC50A

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   CDC50A (TMEM30A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. His-FGE

   Plasmid

   #75147

   Purpose

   Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

   Depositor

   Richard Fishel

   Article

   Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

   Insert

   FGE
   

   Use

   Tags

   Histidine tag and Thrombin

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   T7

   Available since

   July 8, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pBsacA

   Plasmid

   #158650

   Purpose

   Constitutive transcription of sacA crRNA

   Depositor

   Wenjing Cui

   Insert

   sacA crRNA
   

   Use

   CRISPR and Synthetic Biology; Shuttle vector baci…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pveg

   Available since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV-ER-mRFP

   Plasmid

   #231180

   Purpose

   Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, from a lentiviral vector

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   ER-mRFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti6-V5-Topo mCherry

   Plasmid

   #128062

   Purpose

   Lentiviral vector for C terminal mCherry fusions

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB-CRX-T2A-NeuroD1

   Plasmid

   #194607

   Purpose

   Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

   Depositor

   Haruhisa Inoue

   Article

   Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

   Insert

   CRX-T2A-NeuroD1
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCardinal-H1-10

   Plasmid

   #56161

   Purpose

   Localization: Nucleus/Histones, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   H1 (H1f0 Mouse)
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pMRX-IB-HaloTag7-mGFP

   Plasmid

   #184903

   Purpose

   Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   monomeric EGFP
   

   Use

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-AviTag-SnoopLigase

   Plasmid

   #105626

   Purpose

   Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

   Depositor

   Mark Howarth

   Article

   Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

   Insert

   AviTag-SnoopLigase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA4/HisMax-Synectin

    Plasmid

    #35943

    Depositor

    Arie Horowitz

    Article

    Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

    Insert

    Synectin (Gipc1 Mouse)
    

    Use

    Tags

    6xHIS and Xpress

    Expression

    Mammalian

    Mutation

    K111R

    Promoter

    CMV

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pBSFI-myr-akt-T308D/S473D

    Plasmid

    #49192

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    T308D; S473D

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LZRS-DNMT1

    Plasmid

    #24952

    Depositor

    Paul Khavari

    Article

    Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

    Insert

    DNMT1 (DNMT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LentiCRISPRv2puro-Ptgs2_B6

    Plasmid

    #170300

    Purpose

    A knock-out vector for the mouse Ptgs2 in C57BL6

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse tyrosinase gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR-FUSN-mCh-Cry2olig

    Plasmid

    #101224

    Purpose

    FUS(1-214) fused to mCh-Cry2olig

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    FUS(aa1-214) (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-Cry2olig

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5-PLA2G15-6xHis WT

    Plasmid

    #213603

    Purpose

    Expresses PLA2G15-6xHis in mammalian cells

    Depositor

    Monther Abu-Remaileh

    Article

    Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

    Insert

    Phospholipase A2 Group XV (PLA2G15 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry-TIS11B

    Plasmid

    #121140

    Purpose

    Mammalian expression of mCherry tagged TIS11B

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    ZFP36L1 (TIS11B) (ZFP36L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. dCas12Max-CBE

    Plasmid

    #195337

    Purpose

    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized dCas12Max-CBE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N243R, E336R, D1049A

    Promoter

    CBh, CMV, hU6

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR-dSV40-CTLA-4-mGFP

    Plasmid

    #223594

    Purpose

    Lentiviral expression of human CTLA4 with mGFP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CHMP2A_GFP_N_term

    Plasmid

    #31805

    Depositor

    Daniel Gerlich

    Article

    Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC57-sgRNA expression vector

    Plasmid

    #51132

    Purpose

    For in vitro transcription of sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FKBP:FRB-FBAR

    Plasmid

    #223620

    Purpose

    Transient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    FBP17 (FNBP1 Human)
    

    Use

    Tags

    FKBP, TagBFP and mGFP, FRB

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Clover2-H2B-6

    Plasmid

    #56536

    Purpose

    Localization: Nucleus/Histones, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    Clover2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pDONR223-LATS1

    Plasmid

    #23665

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    LATS1 (LATS1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-EF1a-DIO-mCherry

    Plasmid

    #50462

    Purpose

    Double floxed mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHAGE-PIK3CA

    Plasmid

    #116771

    Purpose

    Lentiviral expression of PIK3CA

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWZL Neo Myr Flag PTK2

    Plasmid

    #20610

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pdestMB14_sur5p-dCas9-VP64

    Plasmid

    #177788

    Purpose

    Overexpression of dCas9-VP64 in C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    dCas9-VP64
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p5xUAS-Flag-NPM1c

    Plasmid

    #131823

    Purpose

    4-hydroxytamoxifen inducible vector expressing Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transduction

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSEVA451-Cpf1

    Plasmid

    #192362

    Purpose

    CRISPR-based vector for genome editing

    Depositor

    Juana María Navarro Llorens

    Article

    Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

    Insert

    Cas12a, gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. sgTP53_3

    Plasmid

    #78164

    Purpose

    sgTP53

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

31. mTFP1-N1

    Plasmid

    #54521

    Purpose

    Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Type

    Empty backbone

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AiP15260 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-3XmiR126TS-BGHpA (Alias: CN5260)

    Plasmid

    #224194

    Purpose

    AiE0779m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFG2

    Plasmid

    #87250

    Purpose

    gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    Nat
    

    Use

    Tags

    parS segments

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

    Plasmid

    #170248

    Purpose

    Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    C124S

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. minCMV-1xmNG-dMCP

    Plasmid

    #231091

    Purpose

    Encodes a 1xmNeonGreen-dMCP under control of a weak promoter (minCMV).

    Depositor

    John Ngo

    Insert

    1xmNG-dMCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLKO_HA.V5.VSVg_NGFR

    Plasmid

    #158244

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.V5.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. ABE-RA3.0

    Plasmid

    #208301

    Purpose

    Expresses ABE-RA3.0 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA3.0-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mCherry-mEGFP

    Plasmid

    #127813

    Purpose

    Hetero-dimer mCherry-mEGFP expression vector

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    mCherry-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p7270 MSCV-N-HA-IRES-PURO Empty

    Plasmid

    #163302

    Purpose

    Empty vector control

    Depositor

    Elizabeth White, Peter Howley

    Article

    White et al J Virol. 2014 Aug;88(15):8201-12. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBE14

    Plasmid

    #174653

    Purpose

    In vivo Cre-lox circularization in E. coli

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    Cre
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. TFORF1330

    Plasmid

    #143950

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TFAP2C (TFAP2C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. mCherry-Fis1

    Plasmid

    #182580

    Purpose

    Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    mCherry-Fis1
    

    Use

    Tags

    Fis1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. peYFP-C1-mTOR

    Plasmid

    #73384

    Purpose

    Expresses YFP tagged mTOR

    Depositor

    Taekjip Ha, Jie Chen

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMaCTag-Z07

    Plasmid

    #124790

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeonGreen
    

    Use

    Pcr template

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. YFVdelNS1-mCherry

    Plasmid

    #179951

    Purpose

    pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry
    

    Use

    Transneuronal tracing

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. TFORF2258

    Plasmid

    #142927

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX11 (SOX11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pVL1392 TMEM97

    Plasmid

    #157748

    Purpose

    Baculovirus transfer vectors to create a virus expressing human TMEM97

    Depositor

    Andrew Kruse

    Article

    Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

    Insert

    TMEM97 (TMEM97 Human)
    

    Use

    Tags

    flag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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48. pAM5505

    Plasmid

    #132664

    Purpose

    pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Insert

    MobA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    please see depositor comments below

    Promoter

    p1,p3

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

    Plasmid

    #67986

    Purpose

    Cas9 activity reporter with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. 1056 pSG5L HA PTEN 380-385E

    Plasmid

    #11171

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E4: S380E, T382E, T383E, S385E

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only