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Venus-rab7a

Plasmid

#234743

Purpose

Mammalian expression of late endosome marker Venus-rab7a

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-rab7a (Rab7 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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RNA motif plasmid cloning backbone

Plasmid

#107253

Purpose

RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

RNA motif plasmid cloning backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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tdTomato-Mito-7

Plasmid

#58115

Purpose

Localization: Mitochondria, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Insert

Mito (COX8A Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-puro_G-CEPIA1er

Plasmid

#164590

Purpose

Stable integration and expression of G-CEPIA1er in mammalian cells

Depositor

Joseph Hyser

Article

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

G-CEPIA1er

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-GpNLuc

Plasmid

#135949

Purpose

Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

GpNLuc LumiFluor

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-hc-MYC

Plasmid

#17220

Purpose

Retroviral expression of human cMYC

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

c-MYC (MYC Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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mCh-Sec61 beta

Plasmid

#49155

Purpose

expression of Sec61 beta fused to mCherry, used as a general ER marker

Depositor

Gia Voeltz

Article

Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

Insert

Sec61 beta (SEC61B Human)

Tags

mCherry*

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2

Plasmid

#17218

Purpose

Retroviral expression of human SOX2

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

SOX2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-Hs.cargo(Cre)

Plasmid

#174863

Purpose

Expresses human Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by HsPeg10 UTRs

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-hOCT3/4

Plasmid

#17217

Purpose

Retroviral expression of human OCT3/4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

OCT3/4 (POU5F1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pCAG-mGFP-Actin

Plasmid

#21948

Depositor

Ryohei Yasuda

Article

Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

Insert

betaActin (ACTB Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ManII-SBP-EGFP

Plasmid

#65252

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ST-SBP-EGFP

Plasmid

#65264

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pDY1513 AAVS1 sgRNA

Plasmid

#234832

Purpose

sgRNA for AAVS1 STITCHR insertion

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

AAVS1 sgRNA

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDGE687

Plasmid

#167299

Purpose

Vector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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Man1B1-GFP

Plasmid

#163646

Purpose

Expresses GFP tagged Man1B1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mannosidase alpha class 1B member 1 (MAN1B1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-Ufm1 ∆C3

Plasmid

#133866

Purpose

Expression of human UFM1 mutant lacking the last three C-terminal residues

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Ubiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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OmpA-mNG-sfTq2ox

Plasmid

#124220

Purpose

Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

OmpA-mNG-sfTq2ox

Expression

Bacterial

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-STAG2-wildtype

Plasmid

#31972

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk3a1

Plasmid

#13758

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a1 (MAPK10 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
BFP-Dcp1a

Plasmid

#153973

Purpose

Fluorescent protein fused to mRNA decapping enzyme 1a

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA decapping enzyme 1a (DCP1A Human)

Tags

mTagBFP-c1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV CaMKII:RiboL1-jGCaMP8m

Plasmid

#239668

Purpose

Expresses soma-targeted (ribosome tethered via RiboL1) jGCaMP8m under CaMKII promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV8

Insert

RiboL1-jGCaMP8m

Use

AAV

Tags

6xHis and RiboL1

Expression

Mammalian

Promoter

CaMKII

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-OPHN1

Plasmid

#221310

Purpose

Expression of OPHN1 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

OPHN1 (pLxIS) (Ophn1 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD2

Plasmid

#20184

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 2 intracellular domain (Notch2 Mouse)

Tags

3X FLAG

Expression

Mammalian

Mutation

Contains murine Notch 2 Intracellular Domain (Met…

Promoter

CMV

Available Since

Jan. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1

Plasmid

#13798

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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Ch-gelsolN

Plasmid

#37262

Depositor

Ikuo Wada

Article

Nagaya et al J Cell Biol. 2008 Jan 14;180(1):129-43.

Insert

gelsolin (GSN Human)

Tags

mCherry

Expression

Mammalian

Mutation

deleted amino acids 380-731

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pFSW GAP43-jRGECO1a P2A Syp-Halo

Plasmid

#185565

Purpose

Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

Depositor

Edwin Chapman

Article

Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

Insert

GAP43-jRGECO1a P2A Syp-Halo

Use

Lentiviral

Tags

HaloTag

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk3a2

Plasmid

#13759

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2 (MAPK10 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only