We narrowed to 9,644 results for: SUB

1. pCMV Ub Lex VP16 (F442A) HA (P#1711)

   Plasmid

   #14596

   Depositor

   Matija Peterlin

   Article

   Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

   Insert

   LexA
   

   Tags

   HA, Ubiquitin, and VP16 (F442A)

   Expression

   Mammalian

   Mutation

   The VP16 sequence contains an F442A mutation. The…

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pCpSRb-5

   Plasmid

   #13727

   Depositor

   Thomas Schwartz

   Article

   Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

   Insert

   Signal recognition particle receptor β subunit (SRP102 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   SRβ circularly permuted D210E188 improved solubil…

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDrSR beta deltaN

   Plasmid

   #13730

   Depositor

   Thomas Schwartz

   Article

   Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

   Insert

   Signal recognition particle receptor β subunit
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   expresses residues 60-266 of Danio rerio SRβ with…

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAKW.114A-PKA

   Plasmid

   #154051

   Purpose

   Expresses PKA in E. coli

   Depositor

   Christopher Voigt

   Article

   Wallace et al Advanced Functional Materials 2020

   Insert

   Protein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
   

   Expression

   Bacterial

   Promoter

   pCin

   Availability

   Academic Institutions and Nonprofits only

   

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5. T7-VEE-GFP

   Plasmid

   #58977

   Purpose

   expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   EGFP
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Promoter

   26S subgenomic promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-ExRai-AMPKAR

   Plasmid

   #192446

   Purpose

   Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   ExRai-AMPKAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKB01_mCherry

   Plasmid

   #69104

   Purpose

   Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

   Plasmid

   #89347

   Purpose

   2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

   Depositor

   Ron Vale

   Article

   Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

   Insert

   Jurkat TCRalpha/beta alleles and CD3e and CD3z
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV human IRS-1 CAAX

   Plasmid

   #11238

   Depositor

   Ronald Kahn

   Article

   Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

   Insert

   IRS-1 (IRS1 Human)
   

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   C-terminal prenylation motif makes IRS-1 localize…

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pet-15b-microID

    Plasmid

    #172881

    Purpose

    expresses microID in E. coli

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    microID
    

    Tags

    myc

    Expression

    Bacterial

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSFFV_sfCherry3C(1-10)

    Plasmid

    #117482

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3C(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3C(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    5 substitutions (K45R, G52D, T106A, K182R, N194D)…

    Promoter

    SFFV

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-ExRai-CKAR(T/A)

    Plasmid

    #118410

    Purpose

    Negative-control mutant for ExRai-CKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-CKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. mito-PAGFP

    Plasmid

    #23348

    Purpose

    Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

    Insert

    PAGFP
    

    Tags

    mito

    Expression

    Mammalian

    Available Since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Lyso-ExRai-AMPKAR

    Plasmid

    #192449

    Purpose

    ExRai-AMPKAR targeted to the lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    Lyso-ExRai-AMPKAR
    

    Tags

    Full-length LAMP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Mito-ExRai-AMPKAR

    Plasmid

    #192448

    Purpose

    ExRai-AMPKAR targeted to the mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    Mito-ExRai-AMPKAR
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE2-SpRY-P2A-GFP

    Plasmid

    #159981

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpRY-P2A-GFP
    

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAcGFP1-mito

    Plasmid

    #174180

    Purpose

    Express AcGFP1 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    AcGFP with mitochondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBbB8k-csg-NbGFP

    Plasmid

    #166858

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469

    Insert

    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-TRPV1exCellHalo

    Plasmid

    #220307

    Purpose

    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

    Depositor

    Eric Senning

    Article

    Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

    Insert

    rTRPV1exCellHalo (Trpv1 Rat)
    

    Tags

    circularly permutated Halotag (Deo et al., Nat Ch…

    Expression

    Mammalian

    Mutation

    linker included to insert cpHaloTag (

    Promoter

    CMV promoter

    Available Since

    July 30, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA3.0 FLAG TRPV4

    Plasmid

    #45751

    Depositor

    Robert Lefkowitz

    Article

    Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

    Insert

    TRPV4 (Trpv4 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. RAMA-bio-His

    Plasmid

    #50737

    Purpose

    Expresses enzymatically monobiotinylated full length RAMA ectodomain

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    RAMA
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pet-15b-ultraID

    Plasmid

    #172879

    Purpose

    expresses ultraID in E. coli

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    ultraID
    

    Tags

    myc

    Expression

    Bacterial

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AMPK(T>A)-SPARK

    Plasmid

    #226256

    Purpose

    phospho-null mutant of AMPK-SPARK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK(T>A)-SPARK
    

    Expression

    Mammalian

    Mutation

    substituted Threonine 20 with Alanine

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSFFV_sfCherry3V(1-10)

    Plasmid

    #117483

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3V(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3V(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    6 substitutions (K45R, G52D, L83W, T106A, K182R, …

    Promoter

    SFFV

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Snap-Omp25

    Plasmid

    #69599

    Purpose

    Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    Snap-Omp25
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMALp2x-CARP-1

    Plasmid

    #41727

    Depositor

    Koji Okamoto

    Article

    Nakano et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

    Insert

    clam ADP-ribosylating protein-1
    

    Use

    Bacterial subcloning

    Mutation

    deleted 5'-UTR nucleotides 1-64

    Promoter

    pMB1

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pLyGo-Bs-1

    Plasmid

    #163137

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRluc-LC3wt

    Plasmid

    #105002

    Purpose

    Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

    Depositor

    Marja Jããttelã

    Article

    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Luciferase

    Tags

    Rluc with C124A substitution

    Expression

    Mammalian

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Flag-HA-COPS5

    Plasmid

    #22541

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    COPS5 (COPS5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-PB1-B/BRISBANE/60/2008

    Plasmid

    #187919

    Purpose

    Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 1
    

    Tags

    UNTAGGED

    Promoter

    CMV Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-PM-ExRai-AktAR2

    Plasmid

    #184050

    Purpose

    Plasma membrane-targeted ExRai-AktAR2

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    PM-ExRai-AktAR2
    

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

    Plasmid

    #138989

    Purpose

    IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGal4x5-E4T-G210

    Plasmid

    #171077

    Purpose

    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4-E4-Gless-cassette
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCS2 HRS-RFP

    Plasmid

    #29685

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)
    

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGG-GFP-TurboID-3XNLS

    Plasmid

    #209400

    Purpose

    Transiently expressing and visualizating the subcellular localization of TurboID fused with 3X nuclear localization signal (NLS) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID-3XNLS
    

    Tags

    GFP

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJAB988

    Plasmid

    #133920

    Purpose

    miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter

    Depositor

    Christopher Voigt

    Article

    Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.

    Insert

    miniICE-tet(M)cat(PC194)_PT7-GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-PA-B/BRISBANE/60/2008

    Plasmid

    #187920

    Purpose

    Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase acidic protein
    

    Tags

    UNTAGGED

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFX-mito-mKate2

    Plasmid

    #174178

    Purpose

    Express mKate2 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mKate2 with mitochondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFX-mito-EGFP

    Plasmid

    #159096

    Purpose

    Express EGFP with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with mitochondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBK-CMV-hNHERF2

    Plasmid

    #47801

    Purpose

    Expresses human NHERF2 in mammalian cells

    Depositor

    Randy Hall

    Article

    Lau et al Biochemistry. 2001 Jul 24;40(29):8572-80

    Insert

    NHERF2 (NHERF2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge