We narrowed to 26,826 results for: STI

1. p663-UBC-V5-CDK13_IDG-K

   Plasmid

   #135268

   Purpose

   Gateway destination clone of CDK13 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-CDK13 (CDK13 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-CAMKIIbeta(T287D)

   Plasmid

   #21223

   Depositor

   Tobias Meyer

   Article

   Shen et al Science. 1999 Apr 2. 284(5411):162-6.

   Insert

   CAMKIIbeta (Camk2b Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CAMKIIbeta(T287D) Lab plasmid CF 0001.

   Available Since

   July 29, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pOEM1_pCMV:hShh-pPH:vsvged

   Plasmid

   #111156

   Purpose

   Baculovirus rescue vector, VSVGED pseudotype, hShh

   Depositor

   Elly Tanaka

   Article

   Nacu et al Nature. 2016 May 19;533(7603):407-10. do

   Insert

   hShh (SHH Human)
   

   Use

   Baculoviral rescue shuttle vector

   Expression

   Insect and Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. YFP-Cdc42

   Plasmid

   #11392

   Depositor

   Joel Swanson

   Article

   Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

   Insert

   cell division cycle 42 (CDC42 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   Feb. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. miniCMV eGFP hPGK BSD

   Plasmid

   #188519

   Purpose

   Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMX230

   Plasmid

   #29479

   Depositor

   Linda Wordeman

   Article

   Manning et al Mol Biol Cell. 2007 Aug . 18(8):2970-9.

   Insert

   Kif2B (KIF2B Human)
   

   Tags

   6xHis and EGFP

   Expression

   Mammalian

   Available Since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCTCON2-BYG

   Plasmid

   #158126

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   mTagBFP
   

   Expression

   Yeast

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dimer cleaved

   Plasmid

   #198199

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-T2A-mTurquoise2
   

   Expression

   Mammalian

   Mutation

   A206K both

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMUT2-VtrA-mCherry

   Plasmid

   #192862

   Purpose

   pMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917

   Depositor

   Morten Sommer

   Article

   Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

   Insert

   VtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
   

   Expression

   Bacterial

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. epi-ABEmax

    Plasmid

    #135974

    Purpose

    Expresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

    Depositor

    Yongming Wang

    Article

    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

11. pME-puro-Venus-FLAG-CD59

    Plasmid

    #50377

    Purpose

    Express Venus and FLAG-tagged human CD59, a GPI-AP in mammalian cells

    Depositor

    Reika Watanabe

    Article

    Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

    Insert

    Venus
    

    Expression

    Mammalian

    Promoter

    SR alpha

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

12. Cdk2

    Plasmid

    #1883

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk2 (CDK2 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1/Puro-CAG-VSFP-CR

    Plasmid

    #40257

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    VSFP-CR
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC-GGPPipi

    Plasmid

    #53280

    Purpose

    Contains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p663-UBC-miniTurbo-V5-MAPK15_IDG-K

    Plasmid

    #170581

    Purpose

    Gateway destination clone of MAPK15 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-MAPK15 (MAPK15 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBJ1806 pRS413-COF1RFP

    Plasmid

    #37101

    Depositor

    John Cooper

    Article

    Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

    Insert

    COF1-RFP
    

    Tags

    RFP

    Expression

    Yeast

    Mutation

    Single RFP internal insertion at Cof147T48D with …

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

17. p667-UBC-BCKDK-V5-miniTurbo_IDG-K

    Plasmid

    #158189

    Purpose

    Gateway destination clone of BCKDK (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    BCKDK-V5-miniTurbo (BCKDK Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PMXS-SLC2A3

    Plasmid

    #72877

    Purpose

    expresses SLC2A3 in mammalian cells

    Depositor

    David Sabatini

    Article

    Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

    Insert

    SLC2A3 (SLC2A3 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pFLEX+mCyclin D1

    Plasmid

    #86793

    Purpose

    mammalian expression of cyclin D1

    Depositor

    Martine Roussel, Charles Sherr

    Article

    Matsushime et al Cell. 1991 May 17;65(4):701-13.

    Insert

    cyclin D1 (Ccnd1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKL004

    Plasmid

    #98920

    Purpose

    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    GCaMP6f tethered to mRuby
    

    Tags

    His and mRuby3

    Expression

    Bacterial

    Promoter

    BBa_J23118 - Strong Constitutive

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pKM808

    Plasmid

    #134181

    Purpose

    Lentiviral sgRNA plasmid with blasticidin resistance

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 12URA-B-ScTop2∆CTD

    Plasmid

    #214924

    Purpose

    Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

    Depositor

    James Berger

    Article

    Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

    Insert

    topoisomerase II
    

    Tags

    6x His-TEV

    Expression

    Yeast

    Mutation

    Deleted amino acids 1178-1428

    Promoter

    Gal 1/10

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. p667-UBC-ADCK5-V5-miniTurbo_IDG-K

    Plasmid

    #189909

    Purpose

    Gateway destination clone of ADCK5 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ADCK5 (ADCK5 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EYFP-Rab7

    Plasmid

    #20164

    Depositor

    Xiaowei Zhuang

    Article

    Lakadamyali et al Cell. 2006 Mar 10. 124(5):997-1009.

    Insert

    Rab7 (RAB7A Canis lupus familiaris)
    

    Expression

    Mammalian

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

25. pLG1-puro Non-targeting sgRNA 3

    Plasmid

    #109003

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBPhsFlp2::PEST

    Plasmid

    #63171

    Purpose

    Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    hsFlp2::PEST
    

    Expression

    Insect

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-Blast

    Plasmid

    #205552

    Purpose

    Express EPN24-MCP-p6-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP-p6
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p663-UBC-miniTurbo-V5-MARK1_IDG-K

    Plasmid

    #170582

    Purpose

    Gateway destination clone of MARK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-MARK1 (MARK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA5 Flag-NFRKB

    Plasmid

    #19789

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    NFRKB (NFRKB Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    V1200A

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-N1-mt-ro1GFP

    Plasmid

    #82407

    Purpose

    Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro1GFP
    

    Tags

    leader sequence of the E1α subunit of pyruvate de…

    Expression

    Mammalian

    Mutation

    C48S/F64L/T65S/S147C/Q204C

    Promoter

    CMV

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GreenT-EC - GPI

    Plasmid

    #193946

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC
    

    Tags

    GPI-anchor and Igk_secretion - HA-Flag

    Expression

    Mammalian

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1(+) prME (JEV) sdm2

    Plasmid

    #52881

    Purpose

    expression of Japanese Encephalitis Virus VLPs in mammalian cells

    Depositor

    Yorgo Modis

    Article

    Nour et al PLoS Pathog. 2013;9(9):e1003585. doi: 10

    Insert

    prME (POLY Japanese encephalitis virus)
    

    Expression

    Mammalian

    Mutation

    sdm2

    Promoter

    CMV

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

33. Cdk2-DN

    Plasmid

    #1882

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk2 (CDK2 Human)
    

    Expression

    Mammalian

    Mutation

    D146N, dominant negative

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

34. pGEX-GST-AGO1

    Plasmid

    #24316

    Depositor

    Carl Novina

    Article

    Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

    Insert

    human AGO1 (AGO1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 7, 2010

    Availability

    Academic Institutions and Nonprofits only

35. pEGFP-C1-hAtg14

    Plasmid

    #24295

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    autophagy related genes 14 (ATG14 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PCTAIRE-1-HA

    Plasmid

    #1999

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    PCTAIRE-1 (CDK16 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

37. CAG_mCh_p85a_mCh

    Plasmid

    #199630

    Purpose

    FRET acceptor for IGF1R sensor

    Depositor

    Ryohei Yasuda

    Article

    Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

    Insert

    p85a-SH2 (Pik3r1 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GFP-FAK W266A

    Plasmid

    #186149

    Purpose

    For expressing FAK in mammalian cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEGFP-C1-CaMKIIbeta(K43R)

    Plasmid

    #21225

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CaMKIIbeta(K43R) (Camk2b Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CaMKIIbeta(K43R) Lab plasmid CF 0003.

    Available Since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. IZ501: pMVP/Lenti-DEST

    Plasmid

    #121848

    Purpose

    pMVP destination vector, empty Lentivirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Lentiviral; Pmvp gateway destination vector

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge