We narrowed to 9,644 results for: SUB

1. pK.myc-MYPT1

   Plasmid

   #24101

   Depositor

   Masumi Eto

   Article

   Eto et al Cell Motil Cytoskeleton. 2005 Oct . 62(2

   Insert

   Myosin-targeting subunit-1 (PPP1R12A Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S50G, P415Q

   Available Since

   March 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP6-VEE-IRES-Puro

   Plasmid

   #58971

   Purpose

   noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Type

   Empty backbone

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Promoter

   26S subgenomic promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. siRNA-resistant SigmaR1-mNeonGreen R119A

   Plasmid

   #226572

   Purpose

   Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   R119A

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. siRNA-resistant SigmaR1-mNeonGreen D188A

   Plasmid

   #226573

   Purpose

   Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   D188A

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. siRNA-resistant SigmaR1-mNeonGreen F191A

   Plasmid

   #226571

   Purpose

   Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   F191A

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

   Plasmid

   #187922

   Purpose

   Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase acidic protein
   

   Tags

   3xFLAG

   Promoter

   CMV Promoter

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. T7-RelA

   Plasmid

   #21984

   Depositor

   Warner Greene

   Article

   Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

   Insert

   NFkB p65 subunit (RELA Human)
   

   Tags

   T7 gp10

   Expression

   Mammalian

   Mutation

   For more details for this expression construct,…

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

8. qs5

   Plasmid

   #24498

   Depositor

   Bruce Conklin

   Article

   Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

   Insert

   Gq alpha (Gnaq Mouse)
   

   Tags

   HA (see comments)

   Expression

   Mammalian

   Mutation

   Replacement of the five carboxyl-terminal amino a…

   Available Since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-alpha4 wt

   Plasmid

   #80016

   Purpose

   Mammalian expression of human integrin alpha4

   Depositor

   Chinten James Lim

   Article

   Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

   Insert

   integrin alpha4 (ITGA4 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

10. Cytosolic FAK biosensor

    Plasmid

    #78300

    Purpose

    FRET biosensor. Visualization of cytosolic FAK activity in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Cytosolic FAK biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-ETF1-E55D

    Plasmid

    #130876

    Purpose

    Encodes ETF1 with an E55D substitution

    Depositor

    Prashant Mali

    Article

    Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

    Insert

    Eukaryotic transcritional termination factor ETF1-E55D (ETF1 Human)
    

    Expression

    Mammalian

    Mutation

    E55D

    Promoter

    CAG

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-CRY2-iSH2

    Plasmid

    #66839

    Purpose

    Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.

    Depositor

    Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    iSH2
    

    Tags

    mCherry-CRY2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. G protein alpha-s-HaloTag

    Plasmid

    #66993

    Purpose

    G protein alpha-s internally tagged with HaloTag and EE epitope

    Depositor

    Catherine Berlot

    Insert

    G-alpha-s-EE-HaloTag (Gnas Rat)
    

    Tags

    HaloTag flanked by SGGGGSGGGGS on each side was i…

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-PB2-B/BRISBANE/60/2008

    Plasmid

    #187917

    Purpose

    Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 2
    

    Tags

    UNTAGGED

    Promoter

    CMV Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRES:hTRAAK

    Plasmid

    #133080

    Purpose

    Mammalian expression vector. It will generate the human TRAAK channel full length

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 4 (KCNK4, TRAAK) (KCNK4 Human)
    

    Expression

    Mammalian

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. RA-Sec61β

    Plasmid

    #153978

    Purpose

    Dimerization dependent fluorescent protein RA domain fused to ER protein Sec61β

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    Sec61 Transolocon Subunit Beta (SEC61B Human)
    

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLPP6

    Plasmid

    #209968

    Purpose

    Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter Dropout (sfGFP)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. PGK 4xMito-mEmerald

    Plasmid

    #200430

    Purpose

    Mammalian expression of fluorescently-tagged marker for mitochondria

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    4x mito-targeting sequence

    Promoter

    PGK

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PE2-SpG-P2A-GFP

    Plasmid

    #159980

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpG-P2A-GFP
    

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. SBP-GFP-Tac

    Plasmid

    #162505

    Purpose

    Expresses GFP tagged Tac in a RUSH vector in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    Tac (IL2RA Human)
    

    Tags

    GFP and SBP

    Expression

    Mammalian

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEF-HA-NBP1 (P#1261)

    Plasmid

    #14586

    Depositor

    Matija Peterlin

    Article

    Lu et al Immunity. 1998 May . 8(5):647-56.

    Insert

    ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

22. pET21a - Ec-ClpX

    Plasmid

    #111516

    Purpose

    Expresses E. coli ClpX with a C-terminal His tag

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    ClpX
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pdCas9-omega

    Plasmid

    #114632

    Purpose

    Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

    Depositor

    Anushree Chatterjee

    Article

    Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

    Insert

    dCas9-omega
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pRetroX-Tight-MCS_PGK-GpNLuc

    Plasmid

    #70185

    Purpose

    Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTol2p2A--3mnx1:mTagBFP-NS-MCS

    Plasmid

    #78250

    Purpose

    Can be used to subclone in gene of interest into blue motor neuron expression vector.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Inserts

    -3mnx1 (mnx1 Zebrafish)
    -mTagBFP-NS-MCS
    

    Use

    Zebrafish motor neuron expression

    Promoter

    -3mnx1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRetroX-Tight-MCS_PGK-OgNLuc

    Plasmid

    #70186

    Purpose

    Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. a1Ic-HE3-pcDNA3

    Plasmid

    #45810

    Depositor

    Edward Perez-Reyes

    Article

    Gomora et al Biophys J. 2002 Jul;83(1):229-41.

    Insert

    CACNA1I (CACNA1I Human)
    

    Use

    Making crna for oocyte injections

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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28. pSD057C

    Plasmid

    #218136

    Purpose

    For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved ZF degron SD36.

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD36
    

    Tags

    rpoZ

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-His-hAos1

    Plasmid

    #53135

    Purpose

    E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Aos1 (SAE1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCS2-HA-GAAZFP-FokI-RR

    Plasmid

    #18754

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    zinc finger nuclease
    

    Expression

    Bacterial

    Mutation

    RNA expression vector for expressing the zinc fin…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-nuc-Nb37

    Plasmid

    #205515

    Purpose

    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb37
    

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-sapphireCKAR(T/A)

    Plasmid

    #118469

    Purpose

    Negative-control mutant for sapphireCKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireCKAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR221_SLC22A2

    Plasmid

    #131964

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A2 (SLC22A2 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pcDNA3.1(+)-blueAKAR(T/A)

    Plasmid

    #118471

    Purpose

    Negative-control mutant for blueAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR(T/A)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pENTR-PPP2R2B

    Plasmid

    #16181

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    PP2A regulatory subunit B, beta isoform (PPP2R2B Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

36. pJEC819

    Plasmid

    #190815

    Purpose

    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9-XTEN-rpoA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    XTEN (linker), followed by the N-terminal domain …

    Promoter

    SP30

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pIRES-Ap2a2-FKBP

    Plasmid

    #46944

    Purpose

    Co-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cells

    Depositor

    Margaret Robinson

    Article

    Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

    Insert

    Ap2a2-FKBP
    

    Tags

    Mainly mouse Ap2a2 cDNA, with a fragment of brain…

    Expression

    Mammalian

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Brp-T2A-QF2w-Hsp70-3XP3-dsRed

    Plasmid

    #141094

    Purpose

    Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    bruchpilot
    

    Use

    CRISPR

    Expression

    Insect

    Mutation

    coding sequence downstream of CRISPR cut site, sy…

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pChlamy/Cre

    Plasmid

    #205440

    Purpose

    An expression vector for Cre recombinase (codon usage for C. reinhardtii)

    Depositor

    Masamichi Kamihira

    Article

    Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

    Insert

    Cre recombinase gene
    

    Use

    Chlamydomonas expression

    Promoter

    Heat shock protein 70A (HSP70A)/ribulose bisphosp…

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBRα-σ70 D581G

    Plasmid

    #53732

    Purpose

    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    

    Expression

    Bacterial

    Mutation

    The sigma 70 moiety carries the D581G substitution

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only