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162,322 results

Showing: 7481 - 7520 of 162322 results

  1. EFSp-GFP-YAP (5SA)

    Plasmid
    #174170
    Purpose
    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.
    Depositor
    Rod Bremner
    Article
    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610
    Insert
    YAP (YAP1 Human)
    Use
    Lentiviral
    Tags
    3x FLAG tag
    Expression
    Mammalian
    Mutation
    Five LATS phosphorylation sites (S61, S109, S127,…
    Promoter
    EFS
    Available since
    Sept. 28, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    174170-plasmid-map-sequence-id-343725

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  3. GFP(N-glyc)-Tac-TC

    Plasmid
    #162496
    Purpose
    Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its TMD and cysotol…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162496-plasmid-map-sequence-id-323476

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  4. p3R112C/T180CBM3

    Plasmid
    #85090
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The R112C/T180C variant of PCNA3 fused to P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3
    Promoter
    T7 promoter
    Available since
    Dec. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    85090-plasmid-map-sequence-id-164484

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  7. ELAC1

    Plasmid
    #39097
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ELAC1 (ELAC1 Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. Cilantro 2

    Plasmid
    #74450
    Purpose
    Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Article
    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    74450-plasmid-map-sequence-id-140420

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  11. pAG CMV Empty Puro

    Plasmid
    #237881
    Purpose
    Empty mammalian expression plasmid for cloning untagged proteins
    Depositor
    Addgene Research Program, Meghan Rego
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237881-plasmid-map-sequence-id-469912

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  15. EF1a-FRT-MCS-WPRE-SV40 - GCH15

    Plasmid
    #230822
    Purpose
    AAV cloning vector for Flpo-dependent expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230822-plasmid-map-sequence-id-457143

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  16. EF1a-MCS-WPRE-SV40 - GCH13

    Plasmid
    #230820
    Purpose
    AAV cloning vector for expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230820-plasmid-map-sequence-id-457137

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  19. Lyn11-BirA*-HA

    Plasmid
    #214474
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and Lyn11 (Plasma Membrane Localization Se…
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214474-plasmid-map-sequence-id-425600

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  20. TFORF0579

    Plasmid
    #143887
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF107 (ZNF107 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143887-plasmid-map-sequence-id-404559

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  21. TFORF3423

    Plasmid
    #144899
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF345 (ZNF345 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144899-plasmid-map-sequence-id-404459

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  22. TFORF3293

    Plasmid
    #144769
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF781 (ZNF781 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144769-plasmid-map-sequence-id-404371

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  23. pCAGGS-SMS2-TEV-Twin-Strep

    Plasmid
    #202529
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SGMS2 (SGMS2 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TGA)
    Promoter
    CAG and chicken β-actin promoter
    Available since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202529-plasmid-map-sequence-id-399646

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  26. TFORF0791

    Plasmid
    #143440
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF99 (ZNF99 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143440-plasmid-map-sequence-id-397879

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  27. TFORF2565

    Plasmid
    #144131
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF655 (ZNF655 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144131-plasmid-map-sequence-id-396205

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  28. TFORF0055

    Plasmid
    #143201
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF678 (ZNF678 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143201-plasmid-map-sequence-id-396117

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  29. TFORF0196

    Plasmid
    #143204
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF844 (ZNF844 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143204-plasmid-map-sequence-id-396099

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  30. TFORF2333

    Plasmid
    #142035
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF491 (ZNF491 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142035-plasmid-map-sequence-id-395937

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  31. TFORF2614

    Plasmid
    #142339
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF275 (ZNF275 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142339-plasmid-map-sequence-id-391442

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  32. TFORF3542

    Plasmid
    #145018
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF720 (ZNF720 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145018-plasmid-map-sequence-id-287168

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  33. TFORF3153

    Plasmid
    #144629
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZFP2 (ZFP2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144629-plasmid-map-sequence-id-347671

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Showing: 7481 - 7520 of 162322 results