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Showing: 9401 - 9450 of 167150 results

  2. HSD11B1

    Plasmid
    #39155
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD11B1 (HSD11B1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    T220I; codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. AXBEv2

    Plasmid
    #204606
    Purpose
    Mammalian expression of evolved adenine transversion base editor AXBEv2
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Available Since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204606-plasmid-map-sequence-id-404720

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  10. TFORF1152

    Plasmid
    #142739
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYOCD (MYOCD Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    142739-plasmid-map-sequence-id-462892

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  14. psd44-G12QL

    Plasmid
    #46825
    Purpose
    expression of mutant G protein alpha 12 in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    GNA12 (GNA12 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Q231L (the mutation reduces GTPase activity resul…
    Promoter
    PGK
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46825-plasmid-map-sequence-id-410918

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  16. Perox-GA

    Plasmid
    #209868
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP A
    Tags
    Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    209868-plasmid-map-sequence-id-415634

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  24. JD150

    Plasmid
    #229485
    Purpose
    Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, VA RNA I, E4orf6, L4-22k/33k
    Use
    AAV and Adenoviral
    Expression
    Mammalian
    Promoter
    CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    229485-plasmid-map-sequence-id-455002

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  25. FUW-TIE1-Cherry

    Plasmid
    #119236
    Purpose
    Plasmid encodes full-length human Tie1 gene fused in-frame with Cherry cloned in lentiviral vector.
    Depositor
    Kari Alitalo
    Insert
    TIE1-mCherry (TIE1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Promoter
    P hUbC
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119236-plasmid-map-sequence-id-231690

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  27. pHelper_TS_V2_ampR

    Plasmid
    #214467
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Promoter
    XylS / AraC
    Available Since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    214467-plasmid-map-sequence-id-429338

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  29. AAV-DIO-TC66T-2A-EGFP-2A-oG

    Plasmid
    #178429
    Purpose
    Expresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    Edward Callaway
    Inserts
    TVA950 Glu66→Thr
    Optimized G
    EGFP
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Promoter
    hSyn
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178429-plasmid-map-sequence-id-354737

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  33. pUBC-popTag-QtE-mScarlet

    Plasmid
    #240101
    Purpose
    Mammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.
    Depositor
    Liam Holt
    Article
    Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e
    Insert
    Encapsulin
    Use
    Lentiviral
    Tags
    mScarlet and popZ
    Expression
    Bacterial and Mammalian
    Promoter
    UBC promoter
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    240101-plasmid-map-sequence-id-476613

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  46. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  48. pSLIK sh human Rb 1534 hyg

    Plasmid
    #31500
    Depositor
    Julien Sage
    Insert
    RB shRNA (RB1 Human)
    Use
    Lentiviral and RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    The target sequence for shRB 1534 is GAACGATTATCC…
    Available Since
    Aug. 18, 2011
    Availability
    Academic Institutions and Nonprofits only
    31500-plasmid-map-sequence-id-14427

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Showing: 9401 - 9450 of 167150 results