165,947 results

1. pAAV-Gateway

   Plasmid

   #32671

   Depositor

   Matthew Nolan

   Article

   White et al Front Mol Neurosci. 2011;4:8. Epub 2011

   Type

   Empty backbone

   Use

   AAV; Aav

   Available Since

   June 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-mito-mEm

   Plasmid

   #185596

   Purpose

   Lentiviral construct for expression of mEmerald targeted to mitochondria

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Insert

   mito-mEm
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4 huZAP(S)

   Plasmid

   #45906

   Depositor

   Harmit Malik

   Article

   Kerns et al PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1

   Insert

   ZAP(S) isoform (ZC3HAV1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   M202T, L388F

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-mpeg1-dendra2

   Plasmid

   #51462

   Purpose

   expresses photoconvertible dendra in macrophages

   Depositor

   Anna Huttenlocher

   Article

   Harvie et al Infect Immun. 2013 Jan;81(1):110-21. doi

   Insert

   mpeg-dendra
   

   Expression

   Bacterial

   Promoter

   mpeg1

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYC1640

   Plasmid

   #158719

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe-GFP-IKBalpha-mut (super repressor)

   Plasmid

   #15264

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKB alpha (NFKBIA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Serine 32 to Alanine, Serine 36 to Alanine

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD649-HAsp-KIR3DL2-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156758

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR3DL2 (KIR3DL2 Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL3.7-EF-EYFP-YAP1_WT-PolyA

   Plasmid

   #112284

   Purpose

   Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Mutation

   WT human YAP1-2gamma

   Promoter

   EF-1 alpha

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 13XLexAop2 IVS ChrimsonR mCherry_tr

   Plasmid

   #111548

   Purpose

   Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. tol2-mpx-Dendra2

    Plasmid

    #29574

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al J Leukoc Biol. 2011 May;89(5):661-7. doi

    Insert

    mpx-Dendra 2
    

    Available Since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pET-32a(+)-TGFB1m

    Plasmid

    #120286

    Purpose

    Produce human recombinant mTGFB1 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    monomeric Transforming Growth Factor-β1
    

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promotor

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQFT-mNG-Pcon-Scar

    Plasmid

    #203678

    Purpose

    pQFT-mNG with constitutively expressed mScarlet-I

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Inserts

    mNeonGreen
    mScarlet-I
    

    Expression

    Bacterial

    Promoter

    PQJ and Phyb18

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQFT-mNGsacB

    Plasmid

    #203676

    Purpose

    pQFT with mNeonGreen and sacB under control of PQJ

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Inserts

    mNeonGreen
    sacB
    

    Expression

    Bacterial

    Mutation

    Has synonymous (silent) mutations to remove BsrGI…

    Promoter

    PQJ

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p15_attB(bxb)_lox

    Plasmid

    #183764

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tol2-mpx:Lifeact-Ruby

    Plasmid

    #45246

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

    Insert

    lifeact-ruby
    

    Use

    Zebrafish expression

    Promoter

    mpx

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Casp7-Flag

    Plasmid

    #11815

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 7 (CASP7 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5 FRT/TO Plk4-mCherry

    Plasmid

    #80269

    Purpose

    mammalian expression plasmid for Plk4-mCherry

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. R-pre-GFP

    Plasmid

    #17274

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2006 Jul 21. 313(5785):347-51.

    Insert

    R-pre
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 2, 2008

    Availability

    Academic Institutions and Nonprofits only

19. C1A-C1A-EYFP

    Plasmid

    #61155

    Purpose

    Diacylglycerol (DAG) reporter

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C1A from PKCG amino acids 26-89

    Promoter

    CMV

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. GRK2-CAAX

    Plasmid

    #166224

    Purpose

    GRK2-CAAX mammalian expression plasmid

    Depositor

    Robert Lefkowitz

    Article

    Inglese et al Nature. 1992 Sep 10;359(6391):147-50. do

    Insert

    bovine GRK2-CAAX (GRK2 Bovine)
    

    Tags

    no

    Expression

    Mammalian

    Mutation

    adding CAAX sequence at C terminal

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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