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Showing: 901 - 950 of 2310 results

  1. PB-UniSAM

    Plasmid
    #99866
    Purpose
    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0
    Depositor
    Lesley Forrester
    Article
    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.
    Insert
    UniSAM-mCherry + U6-gRNA2.0
    Use
    CRISPR and Synthetic Biology; Dcas9-sam activation
    Tags
    Expression
    Mammalian
    Mutation
    BbsI sites in CDS were ablated by consensus mutat…
    Promoter
    EF1a
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99866-plasmid-map-sequence-id-192399

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  13. pAC84-pCR8-dCas9

    Plasmid
    #48218
    Purpose
    dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A)
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag
    Expression
    Mutation
    D10A;H840A
    Promoter
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48218-plasmid-map-sequence-id-69495

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  20. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid
    #133347
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_cpaA and sgRNA_blaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133347-plasmid-map-sequence-id-269540

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  24. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid
    #133348
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_blaA and sgRNA_cpaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133348-plasmid-map-sequence-id-267409

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  25. pBHM2646

    Plasmid
    #229530
    Purpose
    Carries mAID_opt tag, empty sgRNA, and NATR marker
    Depositor
    Hiten Madhani
    Insert
    mAID_opt - empty sgRNA - NATR
    Use
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229530-plasmid-map-sequence-id-459934

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  26. pYPQ-CBE-Cas9n-Act3.0

    Plasmid
    #178955
    Purpose
    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178955-plasmid-map-sequence-id-458203

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  27. pYPQ-ABE-Cas9n-Act3.0

    Plasmid
    #178956
    Purpose
    It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178956-plasmid-map-sequence-id-354328

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  30. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid
    #170378
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Article
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin promoter
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    170378-plasmid-map-sequence-id-333532

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  33. pNICKclos1.0

    Plasmid
    #73639
    Purpose
    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    Cas9 nickase
    sGRNA to pyrE
    Use
    E.coli-clostridium shuttle vector
    Tags
    Expression
    Mutation
    D10A
    Promoter
    j23119 and ptb
    Available since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73639-plasmid-map-sequence-id-148603

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  39. pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82705
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    82705-plasmid-map-sequence-id-170450

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  40. pNICKclos2.0

    Plasmid
    #73228
    Purpose
    Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    Cas9 nickase
    sgRNA to xylR
    Use
    E.coli - clostridium shuttle vector
    Tags
    Expression
    Mutation
    D10A
    Promoter
    Pj23119 and Pthl
    Available since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73228-plasmid-map-sequence-id-178086

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  41. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid
    #48226
    Purpose
    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    Use
    CRISPR
    Tags
    2A-puro, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48226-plasmid-map-sequence-id-69514

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  42. pYPQ141-ZmUbi-RZ-As

    Plasmid
    #86196
    Purpose
    AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing
    Depositor
    Yiping Qi
    Article
    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10
    Insert
    AsCpf1 gRNA cloning site for ribozyme cleavage
    Use
    CRISPR
    Tags
    Hammerhead ribozyme and HDV ribozyme
    Expression
    Plant
    Mutation
    Promoter
    Maize ubiquitin 1
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    86196-plasmid-map-sequence-id-176061

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  43. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82706
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82706-plasmid-map-sequence-id-158046

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  44. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82704
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO-L Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82704-plasmid-map-sequence-id-158051

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  45. pYPQ-CBE-SpRYn-Act3.0

    Plasmid
    #178958
    Purpose
    It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178958-plasmid-map-sequence-id-354326

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  47. pYPQ-ABE-SpRYn-Act3.0

    Plasmid
    #178959
    Purpose
    It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178959-plasmid-map-sequence-id-354322

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  48. pNOC_hfnCas12a_NR1_NR2_LPT1

    Plasmid
    #176253
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT gene
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176253-plasmid-map-sequence-id-347028

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  49. pNOC_hfnCas12a_NR1_NR2

    Plasmid
    #176252
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by full
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    Promoter
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    176252-plasmid-map-sequence-id-346964

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Showing: 901 - 950 of 2310 results