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pAAV-FLEx-ER-TurboID

Plasmid

#160857

Purpose

Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Publication

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has service

AAV8

Insert

ER TurboID

Use

AAV

Tags

HA and V5

Promoter

CAG

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEvol-pAzFRS.2.t1

Plasmid

#73546

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Publication

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.2.t1
pAzFRS.2.t1

Expression

Bacterial

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCMV(CAT)T7-SB100

Plasmid

#34879

Depositor

Zsuzsanna Izsvak

Publication

Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

Insert

SB100X transposase

Expression

Mammalian

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

309 Articles

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pEvol-pAzFRS.1.t1

Plasmid

#73547

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Publication

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.1.t1
pAzFRS.1.t1

Expression

Bacterial

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ptfLC3

Plasmid

#21074

Purpose

Mammalian expression of rat LC3 fused to mRFP and EGFP

Depositor

Tamotsu Yoshimori

Publication

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP and mRFP

Expression

Mammalian

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

330 Articles

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pDGM6

Plasmid

#110660

Purpose

Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.

Depositor

David Russell

Publication

Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.

Insert

AAV6 cap genes, AAV2 rep genes, adenovirus helper genes

Use

AAV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pAdTrack-CMV

Plasmid

#16405

Purpose

Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Has service

Cloning Grade DNA

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

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pAdEasy-1

Plasmid

#16400

Purpose

Adenoviral vector for use in AdEasy System.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Has service

Cloning Grade DNA

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pAAV-nEFCas9

Plasmid

#87115

Purpose

SpCas9 expression AAV backbone plasmid under control of nEF promoter

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

nEF-Cas9

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pAAV-hSynapsin-FLEX-soCoChR-GFP

Plasmid

#107712

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virus

Depositor

Edward Boyden

Publication

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Has service

AAV9

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Promoter

hSynapsin

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET16B.Pfu

Plasmid

#12509

Purpose

Bacterial expression of Pfu DNA polymerase

Depositor

Sung-Hou Kim

Publication

Goldman et al Acta Crystallogr D Biol Crystallogr. 199

Insert

Pfu DNA polymerase

Expression

Bacterial

Mutation

Y218H, Q462R, F533L, K559I and V683A (mutations w…

Available since

Sept. 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMitoTimer

Plasmid

#52659

Purpose

Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo

Depositor

Zhen Yan

Publication

Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1

Insert

DsRed1-E5

Use

Dsred

Tags

human cytochrome c oxidase subunit VIII mitochond…

Promoter

CMV

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

44 Articles

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pShuttle-CMV

Plasmid

#16403

Purpose

Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Has service

Cloning Grade DNA

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pMSP1E3D1

Plasmid

#20066

Purpose

Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

Depositor

Stephen Sligar

Publication

Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

Insert

MSP1E3D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeats of …

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

72 Articles

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pmRFP-LC3

Plasmid

#21075

Purpose

Mammalian expression of rat LC3 fused to mRFP

Depositor

Tamotsu Yoshimori

Publication

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

mRFP

Expression

Mammalian

Mutation

First ATG is not included.

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

95 Articles

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pFGET19_Ulp1

Plasmid

#64697

Purpose

H6-Ulp1 from pHYRS52 into pET-28b(+)

Depositor

Hideo Iwai

Publication

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Insert

ULP1 (ULP1 Saccharomyces cerevisiae, Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

Ulp1(403-621)

Promoter

T7

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

32 Articles

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myc-Rictor corrected

Plasmid

#11367

Purpose

Mammalian expression of myc tagged human RICTOR

Depositor

David Sabatini

Publication

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available since

April 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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pET21a-Streptavidin-Alive

Plasmid

#20860

Purpose

Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

Depositor

Alice Ting

Publication

Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

Insert

Streptavidin-Alive

Tags

His

Expression

Bacterial

Mutation

Wt gene

Available since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pShuttle

Plasmid

#16402

Purpose

Shuttle vector for use in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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sortase A pentamutant (eSrtA) in pET29

Plasmid

#75144

Purpose

evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

Depositor

David Liu

Publication

Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

Insert

sortase A pentamutant

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T. Deleted amino a…

Promoter

T7

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

36 Articles

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