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164,577 results

Showing: 7561 - 7600 of 164577 results

  1. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

    Plasmid
    #121966
    Purpose
    Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.
    Depositor
    Clifford Brangwynne
    Article
    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.
    Insert
    scFv-GCN4
    Use
    Lentiviral
    Tags
    superfold GFP-GB1-NLS-iLID
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available Since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    121966-plasmid-map-sequence-id-236121

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  8. pMito-TrxRFP1

    Plasmid
    #98997
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized
    Depositor
    Huiwang Ai
    Article
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Insert
    TrxRFP1 and mitochondrial localization sequence
    Use
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    98997-plasmid-map-sequence-id-189692

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  9. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid
    #140980
    Purpose
    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasS-RLuc8
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140980-plasmid-map-sequence-id-300680

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  16. pDIRECT_23C

    Plasmid
    #91140
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91140-plasmid-map-sequence-id-182268

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  23. DsRed2-Mito-7

    Plasmid
    #55838
    Purpose
    Localization: Mitochondria, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Use
    Tags
    DsRed2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    55838-plasmid-map-sequence-id-244346

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  31. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

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  33. pAAV-hSyn-DIO-EGFP

    Plasmid
    #50457
    Purpose
    Double floxed EGFP under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available Since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50457-plasmid-map-sequence-id-162265

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  37. pGWB402SC

    Plasmid
    #224552
    Purpose
    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.
    Depositor
    Paul Schulze-Lefert
    Article
    Lawson et al bioRxiv 2024.07.14.603419
    Type
    Empty backbone
    Use
    Tags
    Twin-Strep-HA
    Expression
    Plant
    Mutation
    Promoter
    2 X 35S
    Available Since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    224552-plasmid-map-sequence-id-444737

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  38. pGWB402SN

    Plasmid
    #224551
    Purpose
    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.
    Depositor
    Paul Schulze-Lefert
    Article
    Lawson et al bioRxiv 2024.07.14.603419
    Type
    Empty backbone
    Use
    Tags
    HA-Twin-Strep
    Expression
    Plant
    Mutation
    Promoter
    2 X 35S
    Available Since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    224551-plasmid-map-sequence-id-444736

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  39. pcDNA3.1-CMV-CFP;UBC-Cre25nt

    Plasmid
    #65727
    Purpose
    Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.
    Depositor
    Jacco van Rheenen
    Article
    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1
    Inserts
    CMV promoter driving CFP expression
    UBC promoter driving Cre-25nt expression
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and UBC
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    65727-plasmid-map-sequence-id-243566

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Showing: 7561 - 7600 of 164577 results