20,093 results

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GFP-hPIKfyve

Plasmid

#121148

Purpose

Expresses GFP-tagged human PIKfyve in mammalian cells.

Depositor

Geert van den Bogaart

Article

Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

Insert

1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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xCas9 3.6

Plasmid

#108384

Purpose

Mammalian expression vector for xCas9 3.6

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.6

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLCNICK

Plasmid

#84653

Purpose

Genome editing for Lactobacillus casei Lc2W

Depositor

Sheng Yang

Article

Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

Inserts

Cas9 nickase
sgRNA
homology arms of LC2W_2179

Use

CRISPR

Mutation

D10A

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pC017 - LwCas13a guide expression backbone with tRNAval promoter

Plasmid

#91907

Purpose

Backbone for cloning LwCas13a guides under tRNAval promoter

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a Guide site

Expression

Mammalian

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-hERalpha

Plasmid

#101141

Purpose

Mammalian expression of full-length human estrogen receptor-alpha

Depositor

Elizabeth Wilson

Article

Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

Insert

estrogen receptor-alpha (ESR1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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mClY-N1

Plasmid

#90457

Purpose

A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

Depositor

Joseph Santos-Sacchi

Article

Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

Insert

mClY-N1

Expression

Mammalian

Mutation

EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH c-Met

Plasmid

#86498

Purpose

Mammalian expression of human c-Met

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Expression

Mammalian

Promoter

EF1a

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

Plasmid

#67977

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMAZ-SK

Plasmid

#73962

Purpose

self-killing gRNA plasmid pMAZ-SK

Depositor

Alex Nielsen

Article

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Insert

gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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PL552

Plasmid

#68407

Purpose

Donor empty vector with floxed puromycin resistance gene expression cassette

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pLacO-ISce1

Plasmid

#58505

Purpose

provides 256 Lac operator repeats adjacent to the ISce1 restriction site

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Lac repeats adjacent to 6 copies of the ISce1 restriction site

Expression

Bacterial

Promoter

no promoter

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR(T/A)

Plasmid

#64928

Purpose

Negative-control mutant for TORCAR biosensor.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR(T/A) (EIF4EBP1 Synthetic, Human)

Tags

Cerulean and YPet

Expression

Mammalian

Mutation

4EBP1 domain contains T37A and T46A mutations.

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP-3XNLS

Plasmid

#58466

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Utr230 with 3X glycine linker (UTRN Human)
3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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mEos4b-C1

Plasmid

#54812

Purpose

Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Type

Empty backbone

Tags

mEos4b

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2-Cas9

Plasmid

#47322

Purpose

For in vivo expression of Cas9 or generation of Cas9 mRNA

Depositor

Alex Schier

Article

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR; Xenopus and zebrafish expression

Expression

Mammalian

Promoter

SP6

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-BbChT

Plasmid

#45186

Depositor

Dawen Cai, Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Has Service

AAV9

Insert

pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE

Use

AAV; Adeno-associated viral

Expression

Mammalian

Promoter

human EF1a

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 LIC cloning vector (2Bc-T)

Plasmid

#37236

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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FUW-Sox10

Plasmid

#36978

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox10 (SOX10 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CD68 promoter/enhancer

Plasmid

#34837

Depositor

Peter Murray

Article

Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

Insert

CD68 promoter/enhancer -680/+140 (CD68 Human)

Expression

Mammalian

Available Since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only

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CLC-pmCherryC1

Plasmid

#27680

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Clathrin Light Chain (Clta Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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