19,635 results

1. pTRIPZ

   Plasmid

   #206981

   Purpose

   empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

   Depositor

   Ming Chen

   Article

   Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

   Plasmid

   #71485

   Purpose

   Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

   Depositor

   Richard Sherwood

   Article

   Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

   Type

   Empty backbone

   Promoter

   U6

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-TORCAR(T/A)

   Plasmid

   #64928

   Purpose

   Negative-control mutant for TORCAR biosensor.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR(T/A) (EIF4EBP1 Synthetic, Human)
   

   Tags

   Cerulean and YPet

   Expression

   Mammalian

   Mutation

   4EBP1 domain contains T37A and T46A mutations.

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBB550

   Plasmid

   #27396

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

   Insert

   dnaK, dnaJ, GroESL
   

   Expression

   Bacterial

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His10 TEV LIC cloning vector (2B-T-10)

   Plasmid

   #78173

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   10xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR_HNF1A-RE_tBFP_PGK_mCitrine

   Plasmid

   #188387

   Purpose

   Lentiviral vector - tBFP reporter for HNF1A-based SNIPRs with a constitutive mCitrine;

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   HNF1A-RE_tBFP_PGK_mCitrine
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCAR_Cas9-blast_GFP

   Plasmid

   #162074

   Purpose

   3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Inserts

   Cas9-2a-blasticidin S deaminase
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in M. musculus

   Promoter

   EF1a and sv40

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-sgGFP-NT1

   Plasmid

   #46914

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   sgGFP-NT1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV4 p65

   Plasmid

   #21966

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NFkB p65 subunit (RELA Human)
   

   Expression

   Mammalian

   Mutation

   This construct was the product of 3 subcloning pr…

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. c-Flag pcDNA3

    Plasmid

    #20011

    Purpose

    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-cmyc

    Plasmid

    #16011

    Purpose

    mammalian expression of human cmyc

    Depositor

    Wafik El-Deiry

    Article

    Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

    Insert

    cmyc (MYC Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-EGFP-NR2b wt

    Plasmid

    #45447

    Depositor

    Robert Malinow, Andres Barria

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR2B (Grin2b Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid

    #111731

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q145 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB454

    Plasmid

    #74836

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. hZMPSTE24-3FLAG_pQCXIP

    Plasmid

    #89759

    Purpose

    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.

    Depositor

    Martin Dorf

    Article

    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127

    Insert

    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    

    Use

    Retroviral; Bicistronic expression: puromycin re…

    Tags

    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…

    Expression

    Mammalian

    Mutation

    Y253H in ZMPSTE24 (see depositor comments below)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. ZipGFP-Casp3

    Plasmid

    #81241

    Purpose

    A ZipGFP-based Caspase-3 protease reporter T2A mCherry

    Depositor

    Xiaokun Shu

    Article

    To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

    Insert

    ZipGFP with caspase cleavage sequence T2A mCherry
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSC-ISL1-T2A-LHX3

    Plasmid

    #90215

    Purpose

    To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

    Depositor

    Chun-Li Zhang

    Article

    Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

    Insert

    ISL1-T2A-LHX3 (ISL1, LHX3 Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81

    Plasmid

    #100157

    Purpose

    Expresses MUS81

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    MUS81 (MUS81 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-MMLVgag-3xNES-ABE8e-NG

    Plasmid

    #181754

    Purpose

    MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

    Depositor

    David Liu

    Article

    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

    Insert

    MMLVgag-3xNES-ABE8e-NG
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-myc-Miro2

    Plasmid

    #47891

    Purpose

    Expresses myc tagged Miro2

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro2 (RHOT2 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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