We narrowed to 13,228 results for: 109
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Plasmid#41810DepositorInsertH9 Super-2 (IL2 Human)
UseTags8x HisExpressionInsectMutationPhenylalanine 42 to AlaninePromoterPolyhedronAvailable sinceJan. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos
Plasmid#211842PurposePlant transient expression cassette for a Dexamethasone-inducible E3-DART ligaseDepositorInsertsLRR-NEL
GAL4-VP16-GR
UseSynthetic BiologyTagsHA, Myc, and mCherryExpressionPlantMutationPromoter6xUAS-35Smini and pUBQ10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
LC201: pMVP/PB/Puro-DEST
Plasmid#121874PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSNG-P2A-Puro
Plasmid#136900PurposeLentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)DepositorInsertFNLSNG
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationL1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337RPromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Wild-type AdPol vector
Plasmid#131685PurposeA lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymeraseDepositorInsertAdenovirus DNA polymerase
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceJune 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_MCP-RecT
Plasmid#164803Purposemammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLSDepositorInsertRecT
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PprgH-GFPLVA
Plasmid#23340DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus
Plasmid#182344PurposesgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.DepositorInsertSpacer 4
UseTagsExpressionWormMutationPromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1delltaH4
Plasmid#71713PurposeMembrane scaffold protein (MSP), helix 4 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSN075
Plasmid#166732PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKD239-1g-2
Plasmid#90959PurposeExpresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoterDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23100, Bba_J23109, and PttrB185-269Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJEC726
Plasmid#190814PurposeCRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.DepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsExpressionMutationS. pyogenes dCas9 codon optimized for Streptomyce…PromoterSP30Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
mt deltaN20 TFB2MdeltaC3
Plasmid#205062PurposeTFB2M protein expressionDepositorInsertTFB2M (TFB2M Human)
UseTagsExpressionBacterialMutationLacks N-terminal 20 aa and C-terminal 3 aaPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLYS5-MCU-Flag
Plasmid#50054PurposeExpression of human MCU in mammalian cellsDepositorInsertMCU-Flag (MCU Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYFJ16-LplA(W37V) (for E. Coli expression)
Plasmid#34838DepositorInsertLplA W37V (lplA E. Coli)
UseTagsHis TagExpressionBacterialMutationMutated Trp37 to ValPromoterT5 promoterAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZA26
Plasmid#158504PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc (ZAR1 Mustard Weed)
UseTags4xMycExpressionPlantMutationD489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP‐RNASEH2C
Plasmid#108698PurposeFor expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cellsDepositorInsertRNASEH2C (RNASEH2C Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEC819
Plasmid#190815PurposeCRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.DepositorInsertdCas9-XTEN-rpoA
UseCRISPR and Synthetic BiologyTagsXTEN (linker), followed by the N-terminal domain …ExpressionMutationPromoterSP30Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Myo1E-pEGFP
Plasmid#124658PurposeMouse myosin-1e fused with EGFPDepositorInsertMyo1e (Myo1e Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRevTRE-hygro-GFP-Crumbs3a WT
Plasmid#195217PurposeRetroviral Tet response vector containing GFP tagged Crumbs3aDepositorInsertCRB3A (CRB3 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-null-GFPLVA
Plasmid#23338DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTJV1Sc-rpoB
Plasmid#166978PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistanceDepositorInsertsUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23101 and VanCCAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf5(WT)-HA
Plasmid#79427PurposeExpresses C-terminally HA-tagged Arf5(WT) in mammalian cellsDepositorInsertArf5 (ARF5 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hRIP3 dRHIM
Plasmid#208788PurposeInducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2DepositorInsertRIP3 (RIPK3 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationRHIM domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP-NOP58-C1
Plasmid#134537PurposeExpresses YFP-NOP58 fusion proteinDepositorInsertNOP58 (NOP58 Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-2C-NLS in pCSDest
Plasmid#126638PurposeExpresses LbCas12a-2C-NLS in mammalian cellDepositorInsertLbCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM272 - MCS mosTI Pmlc-2_GFP target
Plasmid#159834PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertMCS mosTI Pmlc-2_GFP target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2
Plasmid#182352PurposePan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRedCm
Plasmid#176225PurposeLambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phageDepositorInsertLambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaB and PLAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mNectarine
Plasmid#21717PurposeBacterial expression of pH-sensitive monomeric mNectarine red fluorescent proteinDepositorInsertmNectarine fluorescent proteins
UseTagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…PromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMS17
Plasmid#127088PurposeIntegrating vector containing a tetracycline inducible promoter for use in Streptomyces sp.DepositorInsertstcp830
TetRiS
terminator mmrt
Terminator fdt
UseStreptomyce integration vector derived from phic3…TagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-SpCas9-HF1
Plasmid#108293PurposepX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-AP1/gamma1
Plasmid#218801PurposeMammalian expression of mouse Ap1 g1DepositorInsertAp1 g1 (Ap1g1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
BA31_pU.CAG.SaCas9-D10A.rBGpA
Plasmid#199251PurposeExpression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)DepositorInsertSaCas9.D10A nickase
UseCRISPRTags3XHA epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEM255 - sgRNA mosTI Pmlc-2_GFP
Plasmid#159835PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertsgRNA mosTI Pmlc-2_GFP
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLC133
Plasmid#198639PurposeExpression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.DepositorInsertdCas9
UseTags3x-FLAGExpressionBacterialMutationPromoterpTetAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AZ64_pE.DonorCLYBL.TS
Plasmid#199228PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for EGFP reporter
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCPP5154
Plasmid#128704PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertavrPto
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Emi LC
Plasmid#113666PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with anti-FIX(a) and anti-FX(a) Emi HC's.DepositorInsertEmicizumab light chain
UseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5616
Plasmid#128727PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertavrPtoB
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9-MS2-BB_BbsI
Plasmid#164802PurposeREDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFPDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2
Plasmid#182348PurposemScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCPP3416
Plasmid#128720PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopT1-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5534
Plasmid#128723PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopX1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Map7-fulllength
Plasmid#46076DepositorInsertMap7-fulllength (Map7 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSMP-RING1_2
Plasmid#36356DepositorInsertRING1 (RING1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)
Plasmid#179005PurposeSp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)
Plasmid#179004PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLC-238
Plasmid#73192PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-chpB (chpB )
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9n-MS2-BB_BbsI
Plasmid#164804PurposeREDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFPDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only