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PKMC356_pTRE-ActivinA_IRES_BFP_WPRE

Plasmid

#225355

Purpose

Lentiviral vector for ActivinA and BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Actvin A and TagBFP (Inhba Synthetic, Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGA2ox2:GUS

Plasmid

#108564

Purpose

Arabidopsis transformation, expresses GA2ox2 promoter/GUS fusion to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

GA2ox2 promoter (GA2OX2 Mustard Weed)

Tags

GUS

Expression

Plant

Promoter

GA2ox2

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)

Plasmid

#214878

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF1 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF1-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

Plasmid

#214881

Purpose

Lentiviral vector encoding RfxCas13d targeting B2M guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crB2M-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1P-iCLHN

Plasmid

#66579

Purpose

AAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Nanoluc-HaloTag

Expression

Mammalian

Promoter

CAG

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-NfiA

Plasmid

#64901

Purpose

Inducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.

Depositor

Vania Broccoli

Article

Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

Insert

Nfia (Nfia Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Exo84

Plasmid

#53762

Purpose

pEGFP-C3 plasmid that expresses Exo84

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Exo84 (EXOC8 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only

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-3.6kb human IFN-gamma luc

Plasmid

#17599

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-3.6 kb to +70 of promoter

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS hygro

Plasmid

#158634

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pTet-O-ATOH1-T2A-PuroR

Plasmid

#162342

Purpose

Lentiviral expression of ATOH1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH1 (ATOH1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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phage UbiC NLS HA stdPCP stdGFP

Plasmid

#104099

Purpose

Lentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

stdPCP-stdGFP

Use

Lentiviral

Tags

N-terminal NLS, Two synonymous copies of GFP (C-t…

Expression

Mammalian

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

Plasmid

#214879

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF2-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mPD-L1-T2A-GFP

Plasmid

#205455

Purpose

lentiviral plasmid for expression of mouse PD-L1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mPD-L1 (Cd274 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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ORF3a-EGFP

Plasmid

#165121

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

ORF3a (ORF3a codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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TK-GFP-Nluc-P2A-neo-TK

Plasmid

#134896

Purpose

Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Insert

TK-GFP-Nluc-P2A-Neo-TK

Use

Cryptosporidium expression

Promoter

Cryptosporidium actin and enolase

Available Since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC13-hygro

Plasmid

#231988

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCI neo-hEST2-HA

Plasmid

#1782

Depositor

Bob Weinberg

Article

Meyerson et al Cell 1997 Aug 22;90(4):785-95.

Insert

hTERT (TERT Human)

Tags

HA

Expression

Mammalian

Mutation

Clone is catalytically active but is dead in vivo.

Available Since

July 10, 2005

Availability

Academic Institutions and Nonprofits only
pcdna DSG-2 TS

Plasmid

#89465

Purpose

DSG-2 tension sensor, using TSmod inserted in human DSG-2

Depositor

Daniel Conway

Article

Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

Insert

desmoglein-2 (DSG2 Human)

Expression

Mammalian

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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VE-cad KI gRNA1

Plasmid

#92310

Purpose

CRISPR-GFP-gRNA for cutting VEcad

Depositor

Sean Palecek

Article

Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

Insert

VEcad KI gRNA1 (CDH5 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLX317 TEAD2

Plasmid

#193686

Purpose

Constitutive lentiviral expression of TEAD2

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

TEAD2 (TEAD2 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Rab35 S22N inactive

Plasmid

#47426

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 S22N (RAB35 Human)

Tags

GFP

Expression

Mammalian

Mutation

S22N inactive

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLV_EF1aUBA3_IRES_Puro

Plasmid

#235660

Purpose

human UBA3 cloned into pLV-EF1a-IRES-Puro vector (Addgene: 85132)

Depositor

Lorenz Studer

Article

Saurat et al Cell Stem Cell. 2024 Aug 1;31(8):1162-11

Insert

UBA3 (UBA3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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VE-cad KI gRNA2

Plasmid

#92311

Purpose

CRISPR-GFP-gRNA for cutting VEcad

Depositor

Sean Palecek

Article

Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

Insert

VEcad KI gRNA2 (CDH5 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet-MKK6(DD)-Puro

Plasmid

#86094

Purpose

Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

Depositor

Cindy Miranti

Article

Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

Insert

MKK6 (MAP2K6 Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

Serine 207 and Threonine 211 both changed to Aspa…

Promoter

CMV/TetO

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC49-puro

Plasmid

#231989

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG eGFP NLS

Plasmid

#170830

Purpose

Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

eGFP

Use

CRISPR, Synthetic Biology, and TALEN

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1

Plasmid

#205445

Purpose

human AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

iCasp9-Crry-mCD59-Qa1 SCT

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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HS-GFP-PACT

Plasmid

#105954

Purpose

heat shock inducible transgenesis, PACT is a centriol marker

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

PACT (AKAP9 Human)

Tags

GFP

Expression

Bacterial

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp2-a

Plasmid

#40047

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCDEF3‐hTIM3 (no tag)

Plasmid

#49212

Purpose

expresses human Tim3

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (HAVCR2 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pLX317 SLC2A1

Plasmid

#193685

Purpose

Constitutive lentiviral expression of SLC2A1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

SLC2A1 (SLC2A1 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO human shRNA 1 DEPTOR

Plasmid

#21335

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR shRNA 1 (DEPTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

Plasmid

#165456

Purpose

Inducible ZEB2 expression from AAVS1 locus

Depositor

Madeline Lancaster

Article

Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

Insert

ZEB2 (ZEB2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2

Plasmid

#170823

Purpose

PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d PPP2CA gRNA2

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CDH5-mCitrine-IRES-puro

Plasmid

#85143

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mCitrine (VE-cadherin, C-terminally tagged with mCitrine (CDH5 Human)

Use

Lentiviral

Tags

mCitrine

Promoter

CAG

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPN066

Plasmid

#91601

Purpose

Express sgRNA targeting human DPP4

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DPP4 (DPP4 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEGFP-C3-Sec6

Plasmid

#53757

Purpose

pEGFP-C3 plasmid that expresses Sec6

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec6 (EXOC3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mKate2-P2A-APEX2-TAPP1-PH

Plasmid

#67662

Purpose

Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-TAPP1-PH

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223_HyPer7RgDAAO

Plasmid

#217746

Purpose

Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.

Depositor

Seth Parker

Article

Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

Insert

HyPer7 D amino acid oxidase

Tags

Nuclear export signal

Expression

Mammalian

Mutation

Fused DAAO to the C-terminus of HyPer7 using a Gl…

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Integrin beta5-2XEGFP

Plasmid

#139779

Purpose

Detecting Integrin beta5 by fluorescence microscopy

Depositor

Staffan Stromblad

Article

Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

Insert

Integrin subunit beta5 (ITGB5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 64,611 results for: cell;