159,132 results

1. Bxb1-GT_CAG-mRuby2

   Plasmid

   #229801

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. rAAV2/MG1.1

   Plasmid

   #184540

   Purpose

   AAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsid

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

   Insert

   AAV-MG1.1 capsid
   

   Use

   AAV; Helper vector for packaging

   Tags

   Expression

   Mutation

   changes on AAV9 capsid: K449R, A587L, Q588M; inse…

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pROSA26-1

   Plasmid

   #21714

   Depositor

   Philippe Soriano

   Article

   Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET His6 Mocr TEV LIC cloning vector (1O)

   Plasmid

   #29658

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, Mocr, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTD103luxI_sfGFP

   Plasmid

   #48885

   Purpose

   Positive feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

   Insert

   LuxI, sfGFP, and LuxR
   

   Use

   Synthetic Biology

   Tags

   ssrA tag (AANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   pLux

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETDuet-1_6xHis-TEV-mCh-NDP52

   Plasmid

   #187829

   Purpose

   Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NDP52 (CALCOCO2 Human)
   

   Use

   Tags

   6xHisTag, TEV, and mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG_smFP V5

   Plasmid

   #59758

   Purpose

   encodes multiple copies of an epitope tag V5

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   smFP _V5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiCRISPR v2-sgBAX-1

   Plasmid

   #210013

   Purpose

   knock out BAX in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Apoptosis regulator BAX (BAX Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MSCV PIG Erg

   Plasmid

   #66984

   Purpose

   Retroviral expression plasmid for mouse Erg expression; containing bicistronic IRES-GFP

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Erg (Erg Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   LTR

   Available since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. midSIIN

    Plasmid

    #185665

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-C1-Fibrillarin

    Plasmid

    #26673

    Depositor

    Sui Huang

    Article

    Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGWB455

    Plasmid

    #74837

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF1355

    Plasmid

    #143100

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ILF3 (ILF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEX-YFP-hLC3WT

    Plasmid

    #24989

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a wild type human MAP1-LC3B fused with EYFP (MAP1LC3B Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

15. TRE-KRAB-dCas9-IRES-BFP

    Plasmid

    #85449

    Purpose

    Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    KRAB-dCas9-IRES-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA Tag, and KRAB domain

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid

    #171098

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    XbaI site added upstream of U6 promotor. GtoC mu…

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBI-p73 wt/EGFP

    Plasmid

    #16545

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Insert

    p73 (TP73 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. phage UbiC scAB-GFP

    Plasmid

    #104998

    Purpose

    Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    single chain Antibody against GCN4
    

    Use

    Lentiviral

    Tags

    GB1, HA tag, and sfGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLentiPGK Puro DEST ERKKTRClover

    Plasmid

    #90227

    Purpose

    Lentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF0725

    Plasmid

    #143071

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFKB2 (NFKB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p-dCas9-HDAC1-Hygro

    Plasmid

    #104409

    Purpose

    transient expression of dCas9-HDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. hiSIIN

    Plasmid

    #185662

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p-dCas9-SSAP-MS2-BB_BbsI

    Plasmid

    #183826

    Purpose

    pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

    Insert

    U6-guideRNA-dCas9-T2A-EBFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBH

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-CreEGFP

    Plasmid

    #165140

    Purpose

    Expression of Cre recombinase fused with EGFP in C-terminal

    Depositor

    Daekee Lee, David Threadgill

    Insert

    Cre recombinase
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

    Plasmid

    #224435

    Purpose

    AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mScarlet-KASH
    U6-gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. H9 pMalLP

    Plasmid

    #41811

    Depositor

    Chris Garcia

    Article

    Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

    Insert

    H9 Super-2 (IL2 Human)
    

    Use

    Tags

    3C protease site, 6x His, and Maltose Binding Pro…

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Jan. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pGIPz-mPD-L1

    Plasmid

    #121488

    Purpose

    Expression of mouse PD-L1 WT

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. NPC1 His6 EGFP

    Plasmid

    #53521

    Depositor

    Matthew Scott

    Article

    Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

    Insert

    NPC1
    

    Use

    Tags

    EGFP and His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Flag-Axin1

    Plasmid

    #109370

    Purpose

    Transient over-expression of human Axin1

    Depositor

    Mariann Bienz

    Article

    Fiedler et al Proc Natl Acad Sci U S A. 2011 Feb 1;108

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-CoChR-mRuby2-ST

    Plasmid

    #109131

    Purpose

    Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    CoChR-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. dimSIIN

    Plasmid

    #185661

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAS159

    Plasmid

    #194482

    Purpose

    synAR Cassette

    Depositor

    Ahmad Khalil

    Article

    Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

    Insert

    synAR Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-mCherry-FKBP-GEF(ARHGEF1)

    Plasmid

    #85152

    Purpose

    acute activation of Rho through rapamycin-induced translocation to membrane-localized FRB

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Fusion of mCherry, FKBP, and the DH-domain of ARHGEF1 (amino acids 380-630) (ARHGEF1 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pT3TS-RfxCas13d-NLS-HA

    Plasmid

    #141321

    Purpose

    Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos, Ariel Bazzini

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d (NLS-RfxCas13d-NLS)
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAV-SYN-mBaoJin-oPRE

    Plasmid

    #218683

    Purpose

    AAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

    Depositor

    Bart Nieuwenhuis

    Insert

    mBaoJin
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEMS2232

    Plasmid

    #158401

    Purpose

    High Copy number plasmid containing Ple316 (NR2E1 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple316 (NR2E1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Ple316

    Available since

    Feb. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. px458_2A_GFP_sgRNA_RACK1

    Plasmid

    #127123

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA RACK1 (RACK1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGK401red-­rtetTAK8N, L131L

    Plasmid

    #165968

    Purpose

    Expresses rTetTA (K8N, L131L) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    tetR
    

    Use

    Synthetic Biology

    Tags

    three copies of VP16 (VP48)

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGST2-GST-TEV-NDP52

    Plasmid

    #187828

    Purpose

    Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mDsRed-C1

    Plasmid

    #54502

    Purpose

    Localization: C1 Cloning Vector, Excitation: 557, Emission: 585

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mDsRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only