We narrowed to 48,666 results for: TOR

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pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

Plasmid

#188375

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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HA-GABARAP

Plasmid

#137760

Purpose

Expression of GABARAP

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAP (GABARAP Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCI H2B-RFP

Plasmid

#92398

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

IRES H2B RFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pp-mCAT1

Plasmid

#83948

Purpose

This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

Depositor

Vincent Lotteau, Philippe Mangeot

Article

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

mCAT1 (Slc7a1 )

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-mouse PKA-RIalpha-mEGFP

Plasmid

#45525

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RI alpha tagged by mEGFP

Tags

His

Expression

Mammalian

Available Since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6-150

Plasmid

#91777

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-150

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HLA-Flag-natT1R3

Plasmid

#113950

Purpose

mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R3 (TAS1R3 Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

residues 21-852

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1-WW mutant

Plasmid

#17792

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 to Alanine; Proline 202 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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pRS40B

Plasmid

#35478

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast integrating vector

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pHR_SFFv_4D5-High-CAR_RHL002

Plasmid

#164825

Purpose

Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)

Use

Lentiviral

Promoter

SFFV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE3G-GSX2

Plasmid

#96964

Purpose

Dox-inducible expression of Gsx2 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GSX2 (Gsx2 Mouse)

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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Ii-Str_SBP-mCherry-Ecadherin

Plasmid

#65289

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-HTB

Plasmid

#44187

Depositor

Edward Callaway

Insert

Histone tagged GFP, T2A-TVA-E2A-B19G

Use

AAV; Adeno-associated vector

Tags

GFP

Promoter

EF1a

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only
pRSII316

Plasmid

#35455

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast centromere vector

Promoter

None

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCG-Hc∆18-AA

Plasmid

#86559

Purpose

Plasmid encodes blinded measles virus H glycoprotein

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

Measles virus H glycoprotein

Expression

Mammalian

Mutation

Y463A, R515A

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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LeGO-1xT/BSD

Plasmid

#27353

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, dTomato/BSD-Fusion

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pDRM210 LGP (Luciferase GFP Puro)

Plasmid

#174722

Purpose

Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

Firefly Luciferase
eGFP
pac

Use

Lentiviral and Luciferase

Tags

E2A linker and F2A linker

Expression

Mammalian

Promoter

MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMK392 (mAID-mClover-BSD)

Plasmid

#121193

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-mClover-BS

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTPGI_dCas9

Plasmid

#64279

Purpose

encodes yeast-optimized dCas9 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

Yeast-optimized dCas9

Tags

SV40 NLS

Expression

Yeast

Mutation

D10A & H840A relative to S. pyogenes Cas9

Promoter

pTPGI

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-IRES-Dre

Plasmid

#55633

Purpose

Expresses Dre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Inserts

mCherry
Dre

Use

AAV

Expression

Mammalian

Promoter

Ef1a and Ef1a/IRES

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5M-TIMP3

Plasmid

#31715

Depositor

Rik Derynck

Article

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

TIMP3 (TIMP3 Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pRS40P

Plasmid

#35481

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast integrating vector

Available Since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pDW464

Plasmid

#8845

Depositor

David Waugh

Article

Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

Type

Empty backbone

Tags

biotin acceptor peptide

Expression

Insect

Available Since

Nov. 18, 2005

Availability

Academic Institutions and Nonprofits only

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ABE8.17-m

Plasmid

#136298

Purpose

expresses ABE8.17-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.17-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGreenPuro-shRNA-Stx3S-C4

Plasmid

#99742

Purpose

3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

Depositor

Thomas Weimbs

Article

Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

Insert

shRNA against Stx3S

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FLCN

Plasmid

#72290

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pECIA2

Plasmid

#47032

Purpose

This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.

Depositor

Chris Garcia

Article

Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

Type

Empty backbone

Use

Secreted expression in drosophila culture

Tags

Fc(human IgG1), HRV 3C Protease Cleavage site, He…

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pHPdesteGFP

Plasmid

#24566

Depositor

Ingrid Lohmann

Article

Boy et al Genesis. 2010 Apr 22. [Epub ahead of pri

Insert

Use

Reporter construct

Tags

eGFP

Available Since

May 19, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.iGluSnFR3.v857.PDGFR

Plasmid

#178333

Purpose

Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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