We narrowed to 8,240 results for: FIE

1. Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#1

   Plasmid

   #208387

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#1 gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgZBP1 (Zbp1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#2

   Plasmid

   #208388

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#2 gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgZBP1 (Zbp1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCLXSN(GFP)-HA-Slc1a5 (mouse)

   Plasmid

   #71458

   Purpose

   inducible expression fo Slc1a5 in mammalian cells

   Depositor

   Shao-Cong Sun

   Article

   Nakaya et al Immunity. 2014 May 15;40(5):692-705. doi

   Insert

   Slc1a5 (Slc1a5 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   A85P, L125F and V384A mutations compared to GenBa…

   Promoter

   CMV

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1 (-) hYAP 1-1delta

   Plasmid

   #124142

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Megan Finch-Edmondson, Marius Sudol

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   None (Wild Type)

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSB167 - pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::rLUC-I::pMAS

   Plasmid

   #123200

   Purpose

   binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) and a firefly luciferase (fLUC, with intron) in plants

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   2x35S::fLUC-I::tNOS tMAS::rLUC-I::pMAS
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDN-D2irC6kwh

   Plasmid

   #44725

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

   Inserts

   pCMV-D2i promoter
   mCherry
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Rabbit β-globin intron II and WPRE

   Expression

   Mammalian

   Mutation

   Initiator motif (Inr) displaced relative to pCMV-…

   Promoter

   pCMV-D2i

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDN-T2dMFot

   Plasmid

   #44558

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

   Inserts

   PTETREG promoter
   rtetR-M2::FFF
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

   Promoter

   PTETREG

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)

   Plasmid

   #117856

   Purpose

   This plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.

   Depositor

   C. Ron Yu

   Article

   Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3 3'UTR reporter MUT 1.3 kb CD274 Hs 3'UTR Final

   Plasmid

   #107010

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST17-ALDH1A1

    Plasmid

    #189749

    Purpose

    The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A1 (ALDH1A1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ATP1A1_G6_T804N_v1_Dual_epegRNA_tevopreQ1

    Plasmid

    #187458

    Purpose

    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 epegRNA (tevopreQ1) with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G6 T804N epegRNA (tevopreQ1) (ATP1A1 Human)
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-LAP-KNL1-M3

    Plasmid

    #115896

    Purpose

    Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.

    Depositor

    Geert Kops

    Article

    Vleugel et al J Cell Biol. 2013 Dec 23;203(6):943-55.

    Insert

    KNL1-M3 (KNL1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Fragment of KNL1 containing three KNL1 repeat seq…

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOmnibac_ZZ_linker_ybbR_MAP7

    Plasmid

    #159718

    Purpose

    Expresses MAP7 in Sf9 cells with a ZZ affinity tag and a ybbR tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    MAP7 (Microtubule Associated Protein 7) (MAP7 Human)
    

    Use

    Tags

    ZZ affinity tag, ybbR tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV.CMV.CD86.C-HA

    Plasmid

    #154848

    Purpose

    Expresses murine CD86 protein (T-lymphocyte activation antigen) with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Cluster of Differentiation 86 (Cd86 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)

    Plasmid

    #155194

    Purpose

    Expresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Truncated sequence of THBS1 (expresses Amino Acid…

    Promoter

    CMV

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV.CMV.SV40.THBS1-deltaCC-HA.SV40(polyA)

    Plasmid

    #155195

    Purpose

    Expresses murine Thrombospondin-1 (THBS1) protein with deletion of Coiled Coil domain and HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    THBS1 with deletion of amino acid residues 276-315

    Promoter

    CMV

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDN-D2irG4kwh

    Plasmid

    #44723

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

    Inserts

    pCMV-D2i promoter
    EGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Rabbit β-globin intron II and WPRE

    Expression

    Mammalian

    Mutation

    Initiator motif (Inr) displaced relative to pCMV-…

    Promoter

    pCMV-D2i

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC

    Plasmid

    #140203

    Purpose

    Fungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    crRNA array elcA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PgpdA

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 (-) hYAP 1-2gamma

    Plasmid

    #124145

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP Kind2 S159/181/666A

    Plasmid

    #105310

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666A

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL2-hOct4 DE-SV40-Luc

    Plasmid

    #26279

    Purpose

    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Insert

    Oct4 Distal Enhancer Element (DE) (POU5F1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments below

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFUSEss-CHIg-mG3_CH3-1

    Plasmid

    #105859

    Purpose

    Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH3.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG1, IgG3 (Ighg1, Ighg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hybrid heavy chain of mouse IgG3 with CH3 derived…

    Promoter

    hEF1-HTLV prom

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFUSEss-CHIg-mG3_CH2-1

    Plasmid

    #105857

    Purpose

    Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH2.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG1, IgG3 (Ighg1, Ighg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hybrid heavy chain of mouse IgG3 with CH2 derived…

    Promoter

    hEF1-HTLV prom

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFUSEss-CHIg-mG3_CH1h-1

    Plasmid

    #105856

    Purpose

    Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH1 and hinge.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG1, IgG3 (Ighg1, Ighg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hybrid heavy chain of mouse IgG3 with CH1 and hin…

    Promoter

    hEF1-HTLV prom

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1 (-) hYAP 1-1alpha

    Plasmid

    #124139

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTR-li-p33_RS-CLIP

    Plasmid

    #104634

    Purpose

    Human Invariant chain with a CLIP replacement with in frame restriction sites (KpnI/XhoI)

    Depositor

    Sébastien Walchli

    Article

    Mensali et al Oncoimmunology. 2019 Jan 11;8(3):1558663

    Insert

    modified Invariant chain p33 (CD74 Human)
    

    Use

    Entry vector for gateway system

    Tags

    none

    Expression

    Mutation

    CLIP domain was replaced by 2 restriction sites

    Promoter

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFUSEss-CHIg-mG3_h-1

    Plasmid

    #105861

    Purpose

    Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived hinge.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG1, IgG3 (Ighg1, Ighg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hybrid heavy chain of mouse IgG3 with hinge deriv…

    Promoter

    hEF1-HTLV prom

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBABE (hygro) hYAP 1-1delta

    Plasmid

    #124150

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

    Plasmid

    #89890

    Purpose

    BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    HA-BirA-2A-citrine
    

    Use

    Unspecified

    Tags

    Citrine

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1 (-) hYAP 1-2beta

    Plasmid

    #124144

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1 (-) hYAP 1-1gamma

    Plasmid

    #124141

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA8-Flag-LARS(1-360aa)

    Plasmid

    #139668

    Purpose

    Expresses N-teminal Flag tagged aa 1-360 of LARS1 protein

    Depositor

    Jie Chen

    Article

    Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

    Insert

    Leucyl-tRNA Synthetase (LARS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-mPC1_4077-eGFP-3HA

    Plasmid

    #108390

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-mPC1_4119-3HA

    Plasmid

    #108395

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGMC00035 (aka pMG0784)

    Plasmid

    #210768

    Purpose

    Lentiviral vector to express human ADRM1

    Depositor

    Raj Chari

    Article

    Osei-Amponsa et al Mol Cell. 2024 Feb 1;84(3):522-537.e8. d

    Insert

    ADRM1 (ADRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEMS2374

    Plasmid

    #225631

    Purpose

    High Copy number plasmid containing Ple391 (DRD2 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    Ple391 (DRD2 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEMS2368

    Plasmid

    #225632

    Purpose

    High Copy number plasmid containing Ple385 (GPR6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    Ple385 (GPR6 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEMS2372

    Plasmid

    #225630

    Purpose

    High Copy number plasmid containing Ple389 (ADORA2A MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    Ple389 (ADORA2A Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAG8Ha-His-EWSR1-359-549

    Plasmid

    #195868

    Purpose

    Expresses the RRM-RG2-ZnF (359-549) of EWS

    Depositor

    David Libich

    Article

    Selig et al Biopolymers. 2023 May;114(5):e23536. doi

    Insert

    EWS 359-549 (EWSR1 Human)
    

    Use

    Tags

    His8-linker-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PX459v3-eSpCas9(1.1)

    Plasmid

    #178800

    Purpose

    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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