We narrowed to 44,096 results for: cha

1. CaV1.3e[8a,11,31b,Δ32,42a] mut

   Plasmid

   #26576

   Depositor

   Diane Lipscombe

   Article

   Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

   Insert

   CACNA1D (Cacna1d Rat)
   

   Use

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Contains exons 8a, 11, 31b and 42a.Deletion of ex…

   Promoter

   Available Since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pEF Doc2beta WT GFP

   Plasmid

   #128820

   Purpose

   To visualize Doc2beta WT in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEIAV-DIO-TLoop-CHR2YFP

   Plasmid

   #66798

   Purpose

   expression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vector

   Depositor

   Edward Callaway

   Article

   Cetin et al J Neurophysiol. 2014 May;111(10):2150-9.

   Insert

   Floxed Chr2-YFP
   

   Use

   Lentiviral

   Tags

   citrine

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CMVTetO7 Promoter

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV iGluu

   Plasmid

   #105832

   Purpose

   Fast indicator for extracellular glutamate imaging

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR S72T variant
   

   Use

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Changed Ser 72 to Thr

   Promoter

   CMV

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK5-Flag CASTOR1 I280A

   Plasmid

   #84495

   Purpose

   overexpression of CASTOR1 I280A mutant

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR1 I280A (GATSL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   I280A

   Promoter

   CMV

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

6. MDH1-miR-31-PGK-GFP-Blast

   Plasmid

   #31515

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Insert

   miR-31 (Mir31 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pET30b jGCaMP8f F366H

   Plasmid

   #204139

   Purpose

   Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f F366H
   

   Use

   Tags

   N-terminal His tag

   Expression

   Bacterial

   Mutation

   Phenylalanine 366 changed to histidine

   Promoter

   T7

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Actin binding domain of Ezrin

   Plasmid

   #62381

   Purpose

   Expresses the actin binding domain of ezrin. Amino acids 552-586.

   Depositor

   Guillaume Charras

   Article

   Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

   Insert

   Actin binding domain of ezrin (EZR Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains aa's 552-586

   Promoter

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNH11 (pmyo-2::Arch(D95N)::2xMycTag)

   Plasmid

   #130275

   Purpose

   Expresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.

   Depositor

   Alexander Gottschalk

   Article

   Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

   Insert

   Arch(D95N)
   

   Use

   Tags

   2x Myc-tag

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBabe-Ypet-PBD

    Plasmid

    #24533

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    PAK binding domain (PAK1 Human)
    

    Use

    Retroviral

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1 TREK-2

    Plasmid

    #224769

    Purpose

    In vitro transcription for Oocyte expression of 1 TREK-2

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 10 (Kcnk10 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mApple-M1 SpastinK388R

    Plasmid

    #134463

    Purpose

    M1 SpastinK388R expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lys to Arg at amino acid 388

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. α1Ic-HE2-pcDNA3

    Plasmid

    #45808

    Depositor

    Edward Perez-Reyes

    Article

    Murbartian et al FEBS Lett. 2002 Sep 25;528(1-3):272-8.

    Insert

    α1Ic (Cacna1i Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid

    #128404

    Purpose

    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Exotoxin A (toxA P. aeruginosa)
    

    Use

    Tags

    6xHis tag and DsbA signal sequence for periplasmi…

    Expression

    Bacterial

    Mutation

    242DNNNS246 and 384DQNRT388 engineered glycosylat…

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJT040 lenti MCS pEF mIFP WPRE

    Plasmid

    #161929

    Purpose

    Lentiviral expression of mIFP with multiple cloning site upstream the pEF promoter

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    mIFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBabe-CyPet-Rac1

    Plasmid

    #24534

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Retroviral

    Tags

    CyPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

17. Dendra2-Vinculin-N-21

    Plasmid

    #57749

    Purpose

    Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    NM_003373.3 (VCL Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pNH13 (pmyo-2::QuasAr::mOrange)

    Plasmid

    #130273

    Purpose

    Expresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    QuasAr::mOrange
    

    Use

    Tags

    mOrange

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTrc99S-ssDsbA-TTc-4xDQNAT

    Plasmid

    #128401

    Purpose

    Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin fragment C domain
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-IFITM3-F8-TAG

    Plasmid

    #122475

    Purpose

    expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    IFITM3 (IFITM3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Phenylalanine 8 to amber codon

    Promoter

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Cav2.2e[a10,Δ18a, Δ24a, 31a, 37b, 46]

    Plasmid

    #26567

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Contains exons a10, 31a, 37b, and 46. Deletion…

    Promoter

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

22. pMpGWB122

    Plasmid

    #68576

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

    Plasmid

    #101298

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-698 of alpha-Catenin

    Promoter

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRK5 CASTOR2-Flag Cterm

    Plasmid

    #84490

    Purpose

    overexpression of CASTOR2 with Flag tag

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR2 (GATSL2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pEF NS34a-msGFP-A'Q-mito

    Plasmid

    #158763

    Purpose

    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

    Plasmid

    #26569

    Depositor

    Diane Lipscombe

    Article

    Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 24a. Contains …

    Promoter

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pTA231-p.Syn-PAmCherry1Hfx

    Plasmid

    #164661

    Purpose

    expression of H. volcanii codon-optimized PAmCherry1

    Depositor

    Anita Marchfelder

    Article

    Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

    Insert

    PAmCherry1Hfx
    

    Use

    Archaeal expression

    Tags

    Expression

    Mutation

    codon-optimization for Haloferax volcanii

    Promoter

    p.Syn

    Available Since

    Feb. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mApple-M11-92

    Plasmid

    #134466

    Purpose

    M11-92 expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin Amino Acids 1-92 (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M1 Spastin Amino Acids 1-92

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]

    Plasmid

    #26571

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 31a Contains e…

    Promoter

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

30. HOXA4 (murine) HA-tag pET

    Plasmid

    #8489

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXA4 (Hoxa4 Mouse)
    

    Use

    Tags

    HA and T7

    Expression

    Bacterial

    Mutation

    Thr-2 changed to Ala-2 to form better ribosomal b…

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

31. Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]

    Plasmid

    #26568

    Depositor

    Diane Lipscombe

    Article

    Pan et al J Neurosci. 2000 Jul 1. 20(13):4769-75.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    contains exons a10, 18a, 31a, 37b, and 46. Del…

    Promoter

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

32. mSNX13-PX (558-677)

    Plasmid

    #119094

    Purpose

    Bacterial expression of human phox homology (PX) domain, mSNX13-PX (558-677)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    mSNX13-PX (558-677)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SNX5-PX (26-170)

    Plasmid

    #119086

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX5-PX (26-170)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX5-PX (26-170) (SNX5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCSN061

    Plasmid

    #101725

    Purpose

    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mCherry-MyosinIIC-N-18

    Plasmid

    #55108

    Purpose

    Localization: Cytoskeleton, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

    Insert

    MyosinIIC
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pECE-M2-PAK2 S20A

    Plasmid

    #31681

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    p21-activated protein kinase 2 S20A (PAK2 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 20 to Alanine

    Promoter

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRSETa-mEos3.2-A69T

    Plasmid

    #98571

    Purpose

    photoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    mEos3.2-A69T
    

    Use

    Tags

    7xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. N-BLa 1.1

    Plasmid

    #88994

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    N-BLa 1.1 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p164 Dync1i1.B

    Plasmid

    #26435

    Depositor

    Elizabeth Fisher

    Article

    Kuta et al PLoS One. 2010 . 5(7):e11682.

    Insert

    cytoplasmic dynein 1 intermediate chain 1 isoform B (Dync1i1 Mouse)
    

    Use

    Subcloning and sequencing

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available Since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pEF Doc2alpha WT GFP

    Plasmid

    #128819

    Purpose

    To visualize Doc2alpha WT in PC12 cells

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. SNX6-PX (27-171)

    Plasmid

    #119087

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX6-PX (27-171)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX6-PX (29-170) (SNX6 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. ps226

    Plasmid

    #133557

    Purpose

    RNAi construct for knock-down of CDKN1A (Cip1, p21)

    Depositor

    David Livingston

    Article

    Chan et al Genes Dev. 2005 Jan 15;19(2):196-201. do

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. AAV-GfaABC1D-DreO-4x6T

    Plasmid

    #196413

    Purpose

    Astrocytic expression of Dre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    DreO
    

    Use

    AAV; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. FLAG-PIWI(aa478-860)

    Plasmid

    #21543

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    contain only aa478-860

    Promoter

    Available Since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

45. SH3PXD2A-PX (1-126)

    Plasmid

    #119115

    Purpose

    Bacterial expression of human phox homology (PX) domain, SH3PXD2A-PX (1-126)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SH3PXD2A-PX (1-126) (SH3PXD2A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    May 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. pNH12 (pmyo-2::MacQ::mCitrine)

    Plasmid

    #130274

    Purpose

    Expresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    MacQ::mCitrine
    

    Use

    Tags

    mCitrine

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. PD-L1-miSFIT-1x

    Plasmid

    #124685

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. C-BLa 1.1

    Plasmid

    #88997

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    C-BLa 1.1 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pTG-mTDG-K341R

    Plasmid

    #52267

    Purpose

    Bacterial expression of mouse TDG-K341R with C-terminal GST-tag

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    TDG (Tdg Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K341R, SUMO acceptor site mutation

    Promoter

    T7

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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50. pGP-CMV-jGCaMP8f F366H

    Plasmid

    #204141

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Use

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    CMV

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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