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164,577 results

Showing: 7921 - 7960 of 164577 results

  3. pMSCVhyg-DEST

    Plasmid
    #119747
    Purpose
    Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Hygromycin resistance)
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    119747-plasmid-map-sequence-id-233679

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  5. EB1-tdTomato

    Plasmid
    #50825
    Purpose
    expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs
    Depositor
    Erik Dent
    Insert
    EB1 (Mapre1 Mouse)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    50825-plasmid-map-sequence-id-377726

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  7. pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)

    Plasmid
    #128164
    Purpose
    UV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
    Use
    Synthetic Biology
    Tags
    FLAG and zif268
    Expression
    Bacterial
    Mutation
    Promoter
    UV5
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128164-plasmid-map-sequence-id-267570

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  13. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

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  14. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid
    #128163
    Purpose
    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    ERBIN PDZ domain (ERBIN Synthetic)
    Use
    Synthetic Biology
    Tags
    FLAG and Omega subunit of RNA polymerase
    Expression
    Bacterial
    Mutation
    Promoter
    UV5
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128163-plasmid-map-sequence-id-267568

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  16. AICSDP-10:LMNB1-mEGFP

    Plasmid
    #87422
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87422-plasmid-map-sequence-id-173949

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  18. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid
    #106477
    Purpose
    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106477-plasmid-map-sequence-id-207983

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  19. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid
    #71657
    Purpose
    mammalian expression of eiF4A1
    Depositor
    Gideon Dreyfuss
    Insert
    eiF4A1 (EIF4A1 Human)
    Use
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    aa403 from Ala to Arg
    Promoter
    CMV
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71657-plasmid-map-sequence-id-422687

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  20. pDONR221-SPNS1_STOP

    Plasmid
    #161439
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SPNS1 (SPNS1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161439-plasmid-map-sequence-id-355837

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  25. pE-SUMO CRYAA

    Plasmid
    #80756
    Purpose
    Expression of human alpha A-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYAA (CRYAA Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80756-plasmid-map-sequence-id-153571

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  28. YIp204-PADH1-atTIR1-9myc

    Plasmid
    #99532
    Purpose
    S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)
    Depositor
    Helle Ulrich
    Insert
    TIR1 (TIR1 Mustard Weed)
    Use
    Tags
    9xMyc
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99532-plasmid-map-sequence-id-192011

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  31. pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122

    Plasmid
    #223186
    Purpose
    Plasmid containing the Zfr/YAP5SA/3xMIR122 cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.
    Depositor
    Yuxuan Guo
    Article
    Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10
    Inserts
    YAP5SA (Yap1 Mouse)
    mir122 (Mir122 Mouse)
    Use
    AAV
    Tags
    HA and engineered P2A
    Expression
    Mutation
    S61A, S109A, S127A, S164A, S381A
    Promoter
    TNNT2
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    223186-plasmid-map-sequence-id-443750

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Showing: 7921 - 7960 of 164577 results