162,309 results

1. pcDNA3.1/CALR wt-FLAG

   Plasmid

   #204532

   Purpose

   Mammalian expression of human CALR wt-FLAG

   Depositor

   Norio Komatsu

   Article

   Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

   Insert

   CALR wt-FLAG (CALR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. piRFP-N3-Tyrosinase

   Plasmid

   #80152

   Purpose

   Melanosome marker

   Depositor

   Santiago Di Pietro

   Article

   Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

   Insert

   Tyrosinase (TYR Human)
   

   Use

   Tags

   iRFP682

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. ICAM2-bio-His

   Plasmid

   #51758

   Purpose

   Expresses full-length Intercellular adhesion molecule 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   ICAM2 (ICAM2 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Px461-Cas9n-Trp53-sgRNA-beta

   Plasmid

   #88847

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-DN-hCUL1-FLAG

   Plasmid

   #15818

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin1 (CUL1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N452

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pcr4-12xMBS-PBS

   Plasmid

   #52984

   Purpose

   provide MBS-PBS alternating sequence

   Depositor

   Robert Singer

   Article

   Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

   Insert

   12xMBS-PBS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-Q23

   Plasmid

   #40261

   Depositor

   David Rubinsztein

   Article

   Narain et al J Med Genet. 1999 Oct;36(10):739-46.

   Insert

   HTT partial exon 1 Q23 (HTT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   expanded poly-Q

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEG302 22aa SunTag NtDRMcd nog

   Plasmid

   #115488

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   NOS_NLS_GB1_NLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pN1/G3BP1-iRFP

   Plasmid

   #129339

   Purpose

   Stress granule marker

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   iRFP (from Dr. M. Matsuda)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-jRCaMP1b

    Plasmid

    #233207

    Purpose

    Retroviral expression of jRCaMP1b calcium reporter

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

    Insert

    jRCaMP1b
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease

    Plasmid

    #228637

    Purpose

    Bacterial expression of the Coxsackievirus 16 3C protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Coxsackievirus 16 3C protease
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. moxBFP

    Plasmid

    #68064

    Purpose

    C-terminal fusion to oxBFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    moxBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. IL6R PLAK1

    Plasmid

    #161518

    Purpose

    Expresses IL6 receptor (full length) in mammalian cells.

    Depositor

    Yoav Shaul

    Article

    Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

    Insert

    Interleukin 6 Receptor (IL6R Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE-mKeima-LGALS3

    Plasmid

    #175780

    Purpose

    Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic flux

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-CAG-FLEX-BFP2-(mCherry)’

    Plasmid

    #115234

    Purpose

    Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    mTagBFP2 and mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SnapTag-Binder

    Plasmid

    #179142

    Purpose

    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    SspB
    

    Use

    Tags

    SNAP-tag

    Expression

    Mammalian

    Mutation

    Y11K/A15E, residue numbering from pdb: 1ou9

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Ddo_sfrp-1

    Plasmid

    #107637

    Purpose

    In situ hybridization and RNAi studies in planarians

    Depositor

    Alejandro Sánchez

    Article

    Accorsi et al The American Biology Teacher 79(3):208-2

    Insert

    sfrp-1
    

    Use

    RNAi; In situ hybridization

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HRE-oUnaG

    Plasmid

    #201712

    Purpose

    Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUCmini-iCAP-AAV1.X1

    Plasmid

    #209780

    Purpose

    non-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

    Insert

    Synthetic construct isolate AAV1-X1 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CAG-LiLac

    Plasmid

    #184570

    Purpose

    Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

    Depositor

    Gary Yellen

    Article

    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

    Insert

    LiLac fluorescent sensor of lactate
    

    Use

    AAV

    Tags

    His7

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pH6HTC_ENO1-Halo

    Plasmid

    #175330

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    ENO1 (ENO1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Tag5Amyc-GSK3b WT

    Plasmid

    #16260

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    GSK3 beta (GSK3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-cFos-tTA-pA

    Plasmid

    #66794

    Purpose

    Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

    Depositor

    William Wisden

    Article

    Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

    Insert

    cfos promoter linked to the tet activator gene
    

    Use

    AAV

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 2Flag-pDEST-N

    Plasmid

    #118371

    Purpose

    Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

27. pE-35S-miniSdd7-nCas9-PBE-GmPPO2

    Plasmid

    #204855

    Purpose

    For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI-mScarlet
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    35S, AtU6-26p

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PGK-H2BmCherry

    Plasmid

    #21217

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTACR TH1-p2aGFP

    Plasmid

    #135815

    Purpose

    CRISPR based on px330 p2aGFP targeting TH gene near stop codon

    Depositor

    Tim Ahfeldt, Lee Rubin

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH 1 guide
    

    Use

    CRISPR

    Tags

    p2a Myr-eGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTACR TH2-p2aGFP

    Plasmid

    #135816

    Purpose

    CRISPR based on px330 p2aGFP targeting TH gene near stop codon

    Depositor

    Tim Ahfeldt, Lee Rubin

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH 2 guide
    

    Use

    CRISPR

    Tags

    p2a Myr-eGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CuO-LacI-NLS–SNAPf

    Plasmid

    #227368

    Purpose

    Lentiviral expression of LacI–SNAP

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    LacI NLS SNAPf
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAd5-B1

    Plasmid

    #122551

    Purpose

    Block 1 for AdenoBuilder genome assembly

    Depositor

    Fred Bunz

    Article

    Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

    Insert

    Adenovirus 5 genomic region 1-3759
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGT-Kh2

    Plasmid

    #122587

    Purpose

    MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    beta-lactamase/sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mCherry-LaminA-C-18

    Plasmid

    #55068

    Purpose

    Localization: Nuclear Envelope, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Lamin A (LMNA Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. iOn-CAG∞RFP

    Plasmid

    #154014

    Purpose

    Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HotPETase_pET21b

    Plasmid

    #215407

    Purpose

    Expression construct for HotPETase gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    HotPETase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDEST40-2XHA-Src-K298M

    Plasmid

    #140314

    Purpose

    Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti.PGK.blast-Renilla_Luciferase

    Plasmid

    #74444

    Purpose

    Control lentiviral vector expressing Renilla luciferase

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Renilla Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmTurquoise2-Tubulin

    Plasmid

    #36202

    Purpose

    In vivo visualization of microtubules (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    Alpha-tubulin (TUBA1B Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCITF-KCC2

    Plasmid

    #61404

    Purpose

    Mammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequence

    Depositor

    Franck Polleux

    Article

    Bortone et al Neuron. 2009 Apr 16;62(1):53-71. doi: 10

    Insert

    KCC2 (SLC12A5 Human)
    

    Use

    Tags

    IRES-tdTomato-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge