-
Plasmid#197902PurposeFor the stable knockdown experiments using shRNA, a complementary sequence (HSALNG0104472-163) targeting the HSALNG0104472 transcript was inserted.DepositorInsertHSALNG0104472-163 (HSALNG0104472-scramble-sequence)
UseLentiviralTagszsGreen1ExpressionMammalianMutation15q11.2 deletionPromoterAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WT
Plasmid#222649PurposeFor in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-termDepositorInsertZc3h12a (Zc3h12a Mouse)
UseIn vitro transcriptionTagsFLAG and HAExpressionMutationPromoterT7Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]
Plasmid#196883PurposeDual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporterDepositorInsertroxSTOProx-ZsGreen-bGH
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH]
Plasmid#196886PurposeDual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporterDepositorInsertroxSTOProx-ZsGreen-bGH
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1
Plasmid#153062PurposeLentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297)DepositorInsertTruncated form of human BHLHE40 (aa 1-297) (BHLHE40 Human)
UseLentiviralTagsExpressionMammalianMutationMutagenesis to insert a stop codon at aa 298 in B…PromoterEF1αAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141N
Plasmid#222650PurposeFor in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-termDepositorInsertZc3h12a (Zc3h12a Mouse)
UseIn vitro transcriptionTagsFLAG and HAExpressionMutationmutated aspartic acid 141 to asparagine to abroga…PromoterT7Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR
Plasmid#207135PurposeDNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-inDepositorInsert5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR
Plasmid#207136PurposeDNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-inDepositorInsert5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh1
Plasmid#123324PurposeLentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertshRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-IKZF3 sh1
Plasmid#123326PurposeLentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertshRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh2
Plasmid#123325PurposeLentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertshRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZSGreen-P2A-Puro-IKZF3 sh2
Plasmid#123327PurposeLentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertshRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-rox-STOP-rox-tRFP-pA-lox-ZsGreen-pA
Plasmid#175438Purposedual plasmid labelling cells of different neuronal population with different fluorophoresDepositorInsertrox-STOP-rox-tRFP-pA
UseCre/Lox; Dre/roxTagsZsGreen on backboneExpressionMammalianMutationPromoterpCAGAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1
Plasmid#138417PurposeCAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1DepositorInsertsBirA
dCas9
UseLentiviralTagsFLAG, Avi-tag and V5, HisExpressionMutationPromoterEF1aAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-mCD8
Plasmid#162747PurposeNFAT-driven ZsGreen-1 reporter gene with mouse CD8 geneDepositorInsert8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVID
Plasmid#236635PurposeCRISPR cloning, gRNA expression, and detection of undesired vector insertion during HDR for DrosophilaDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterdU6:3Available sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_4
Plasmid#192688PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_1
Plasmid#192685PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_2
Plasmid#192686PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_3
Plasmid#192687PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-hCD4
Plasmid#162746PurposeNFAT-driven ZsGreen-1 reporter gene with human CD4 geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-muhCD4
Plasmid#162745PurposeNFAT-driven ZsGreen-1 reporter gene with mutated human CD4 geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
UseRetroviralTagsExpressionMammalianMutationHuman CD4 gene with glutamine to tyrosine at posi…PromoterAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-anti-MIR328-3p
Plasmid#153319PurposeExpresses zsGreen fused to a miR-328-3p sponge and mCherry in mammalian cells.DepositorInsertzsGreen with MIR328-3p sponge
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-anti-MIR211-5p
Plasmid#153318PurposeExpresses zsGreen fused to a miR-211-5p sponge and mCherry in mammalian cells.DepositorInsertzsGreen with MIR211-5p sponge
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-hCD8
Plasmid#153417PurposeNFAT-driven ZsGreen-1 reporter gene with human CD8ab geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR
Plasmid#204146PurposeLentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genesDepositorInsertsSARS-CoV-2 BA.1 variant spike
zsGreen-T2A-PuR
UseLentiviralTagsExpressionMammalianMutation21 amino acid cytoplasmic tail deletionPromoterCMV and TRE3GAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIV-H2BmRFP
Plasmid#18982DepositorInsertHistone H2B monomeric red fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
p23-DDX21 WT
Plasmid#128803Purposeoverexpression of DDX21 in human cellsDepositorInsertDDX21 WT
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ai6
Plasmid#22798DepositorInsertCAG-Floxed ZsGreen
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHIV-Wnt1HA
Plasmid#18983DepositorInsertwingless-related MMTV integration site 1 (Wnt1 Mouse)
UseLentiviralTagsHA-TagExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-GFP
Plasmid#106281PurposeA version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.DepositorInsertCMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p23-DDX21 SAT
Plasmid#128804Purposeoverexpression of DDX21 SAT mutation in human cellsDepositorInsertDDX21 SAT mutation
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
p23-DDX21 DEV
Plasmid#128805Purposeoverexpression of DDX21 DEV mutation in human cellsDepositorInsertDDX21 DEV mutation
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV2_HLF-ZE
Plasmid#175034PurposeHLF targeting construct introducing an ires ZsG P2A tEGFR cassette downstream of the human HLF stop codonDepositorInsertHLF ires ZsGreen P2A tEGFR
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV2_HLF-ZP
Plasmid#175035PurposeHLF targeting construct introducing an ires ZsG P2A Puro cassette downstream of the human HLF stop codonDepositorInsertHLF ires ZsGreen P2A Puro
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
VPR_dCas9
Plasmid#134601PurposeExpress CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 markerDepositorInsertVPR-dCas9-2A-ZsGreen-DR
UseTagsVPR and ZsGreen1-DRExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
LEPZ
Plasmid#111161PurposemiR-E (miR-30 variant)-based RNAi with ZsGreen reporterDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsExpressionMutationWTPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-GCaMP6m-XC
Plasmid#118975PurposeImproved genetic encoded calcium sensor based on GCaMP6mDepositorInsertGCaMP6m-XC
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha-EB3-NeonGreen
Plasmid#175263Purposelabelling of microtubule plus end and F-actin structuresDepositorInsertEB3 (MAPRE3 Human)
UseTagsmNeonGreenExpressionMammalianMutationNone (wt)PromoterpTub-alpha1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
p23-puro-EGFP-RPA194
Plasmid#179237Purposeoverexpression in human cellsDepositorInsertRPA194 (POLR1A Human)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
LVDP-CArG-RE-GPR
Plasmid#89762PurposeQuantitative measurement of CArG response element activity.DepositorInsert6X CArG-RE
UseLentiviralTagsCMV-ZsGreenExpressionMammalianMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p23-puro-EGFP-FBL
Plasmid#179235Purposeoverexpression in human cellsDepositorInsertFBL (FBL Human)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-FLAG-HA-Regnase-3 D252N
Plasmid#222652PurposeFor in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-termDepositorInsertZc3h12c (Zc3h12c Mouse)
UseIn vitro transcriptionTagsFLAG and HAExpressionMutationmutated aspartic acid 252 to asparagine to abroga…PromoterT7Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-FLAG-HA-Regnase-3 WT
Plasmid#222651PurposeFor in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-termDepositorInsertZc3h12c (Zc3h12c Mouse)
UseIn vitro transcriptionTagsFLAG and HAExpressionMutationPromoterT7Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-CSNK1A1 G40N-P2A-Blast
Plasmid#123321PurposeLentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Blast casette from a CMV promoterDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationchanged Glycine 40 to AsparaginePromoterCMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 WT
Plasmid#179228Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmNeongreenExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR
Plasmid#204151PurposeLentivirus backbone with Delta spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genesDepositorInsertSARS-CoV-2 Delta variant spike
UseLentiviralTagsExpressionMammalianMutation21 amino acid cytoplasmic tail deletionPromoterTRE3GAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 WT-mRuby3
Plasmid#179225Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmNeongreen and mRuby3ExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p23-mRuby3-DDX21 WT
Plasmid#179231Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmRuby3ExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p13xQUAS-MCS, α-cry:mCherry
Plasmid#180481PurposeInducible gene expression vector p13xQUAS-MCS, α-cry:mCherryDepositorInsertMultiple cloning site
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only