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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,214 results for: LEA

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Showing: 21 - 40 of 12214 results

  2. pSm-Nuclease

    Plasmid
    #132431
    Purpose
    Expresses Serratia marcescens nuclease in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    Serratia marcescens nuclease (codon optimized)
    Tags
    None
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132431-plasmid-map-sequence-id-258770

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  6. pCMV-CIB1-mCerulean-MP

    Plasmid
    #58366
    Purpose
    For use in light-induced protein inactivation through the interaction with CRY2
    Depositor
    Won Do Heo
    Publication
    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CIB1[1-147] (CIB1 Mustard Weed)
    Camk2a[315-478] (Camk2a Rat)
    Tags
    mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58366-plasmid-map-sequence-id-90201

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  7. nuclear NAD+ biosensor

    Plasmid
    #186789
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus
    Depositor
    Michael Cohen
    Publication
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    nuclear NAD+ biosensor
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    186789-plasmid-map-sequence-id-368062

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  9. pCS-H2B-cerulean

    Plasmid
    #53748
    Purpose
    Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.
    Depositor
    Sean Megason
    Publication
    Megason et al Methods Mol Biol. 2009;546:317-32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    histone H2B (H2BC21 Human)
    Tags
    Cerulean
    Expression
    Mammalian
    Promoter
    simian CMV IE94
    Available since
    July 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53748-plasmid-map-sequence-id-83988

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  16. eSpCas9(1.1)

    Plasmid
    #71814
    Purpose
    Expresses high specificity SpCas9. Px330-like plasmid.
    Depositor
    Feng Zhang
    Publication
    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71814-plasmid-map-sequence-id-177023

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  19. pAG/MNase

    Plasmid
    #123461
    Purpose
    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
    Depositor
    Steven Henikoff
    Publication
    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1
    Insert
    Protein A-Protein G-Micrococcal Nuclease
    Tags
    6xHIS and HA
    Expression
    Bacterial
    Promoter
    Lac
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123461-plasmid-map-sequence-id-417805

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  20. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72247-plasmid-map-sequence-id-485302

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Showing: 21 - 40 of 12214 results