163,445 results

1. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216251

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgSEC61B.N1 (SEC61B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYLL201

   Plasmid

   #53454

   Purpose

   Expresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)

   Depositor

   Lisa Lyu, Leroy Liu

   Article

   Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

   Insert

   HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQF

   Plasmid

   #48095

   Purpose

   Cumate-inducible expression of 3xFLAG-tagged fusion protein

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   PQ5

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-N 16E5

   Plasmid

   #37874

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   HPV 16 E5 (E5 )
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   PKG

   Available since

   July 25, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pTXB1-pA-DddA11_NT

   Plasmid

   #232443

   Purpose

   ProteinA-DddA11_NT for D&Dseq (sc TFs molecular footprint)

   Depositor

   Ivan Raimondi

   Article

   Chi et al bioRxiv [Preprint]. 2025 Jan 2:2024.12.3

   Insert

   pA-DddA_NT
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Hygro(+)-PSTCD-cGAS

   Plasmid

   #102605

   Purpose

   Mammalian expression of cGAS fused to PSTCD tag

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Insert

   cGAS (CGAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-SLCO2B1_STOP

   Plasmid

   #161032

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLCO2B1 (SLCO2B1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCAG-YTB

   Plasmid

   #26721

   Depositor

   Edward Callaway

   Article

   Marshel et al Neuron. 2010 Aug 26. 67(4):562-74.

   Insert

   venusYFP, TVA950, Rabies Glycoprotein (RABVgp4, CD320 Chicken, Rabies virus (strain SAD B19))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRST7-ER beta (long)

   Plasmid

   #11356

   Depositor

   Donald McDonnell

   Article

   Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

   Insert

   ER beta long (ESR2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pINTO-NFH::hSNRNP70

    Plasmid

    #65926

    Purpose

    Expression of human SNRNP70 fused to Flag-HA tag (N-terminus)

    Depositor

    Roberto Bonasio

    Article

    Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

    Insert

    small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Two silent mutations

    Promoter

    CMV/TO and CMV/TetO2 (T-REx)

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS/ES-EnvA-VSVg-WPRE-pA

    Plasmid

    #87221

    Purpose

    to package wild type EnvA psudotyped lentivirus

    Depositor

    Fan Wang

    Article

    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

    Insert

    EnvA-VSVg
    

    Use

    Mouse Targeting and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. GluA1 (px330:2-guideCas9)

    Plasmid

    #169417

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-hSyn-Mito-HyPerGRt

    Plasmid

    #173198

    Purpose

    AAV transfer plasmid for a HyPer7 and tdTomato fusion under hSyn promoter

    Depositor

    Huiwang Ai

    Article

    Li et al ACS Sens. 2022 Feb 28. doi: 10.1021/acss

    Insert

    Hyper7-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMiniT:SEP-GluA1 TKIT donor

    Plasmid

    #169418

    Purpose

    Donor DNA for TKIT knockin of SEP to GluA1 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-FRT-EF1a-TVA-2A-N2cG

    Plasmid

    #172361

    Purpose

    Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-N2cG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AiP12857 - pAAV-AiE0674m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2857)

    Plasmid

    #224013

    Purpose

    AiE0674m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SuExp-hPLD3

    Plasmid

    #201243

    Purpose

    express full length human PLD3 protein

    Depositor

    David Nemazee

    Article

    Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

    Insert

    PLD3 (PLD3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSV2 neuNT

    Plasmid

    #10918

    Depositor

    Bob Weinberg

    Article

    Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

    Insert

    neuNT (Erbb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

19. SAP145

    Plasmid

    #11239

    Depositor

    Robin Reed

    Article

    Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

    Insert

    SAP145 (SF3B2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pGEX2T E7

    Plasmid

    #13634

    Depositor

    Karl Munger

    Article

    Wu et al J Virol. 1993 Apr . 67(4):2402-7.

    Insert

    HPV 16 E7 (E7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only