Skip to main content
Addgene
Showing: 951 - 1000 of 2950 results

  1. pEF NS34a-msGFP-'S-mito

    Plasmid
    #158765
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158765-plasmid-map-sequence-id-313128

    Enlarge

  5. pDOC-K-glmS-mPlum

    Plasmid
    #158062
    Purpose
    Donor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable marker
    Depositor
    Mark Webber
    Article
    Holden et al Microbiology (Reading). 2020 Nov 23. doi
    Insert
    monomeric plum fluoresecent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    acpP
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    158062-plasmid-map-sequence-id-309442

    Enlarge

  11. Fast-GCaMP-EF05

    Plasmid
    #52558
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-EF05
    Use
    Tags
    Expression
    Insect
    Mutation
    changed from GCaMP3: D397N/G398A/N399D
    Promoter
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. Fast-GCaMP-RS15

    Plasmid
    #52652
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted G47/H48 from GCaMP3
    Promoter
    T7
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. Fast-GCaMP-RS06

    Plasmid
    #52647
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS06
    Use
    Tags
    Expression
    Mammalian
    Mutation
    M374Q mutated from GCaMP3
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. Fast-GCaMP-RS14

    Plasmid
    #52651
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS14
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted G47 from GCaMP3
    Promoter
    T7
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  31. Fast-GCaMP-RS12

    Plasmid
    #52650
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS12
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted R40/R41/K42 from GCaMP3
    Promoter
    T7
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. Fast-GCaMP-EF09

    Plasmid
    #52640
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-EF09
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D366N mutation from GCaMP3 sequence
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. Fast-GCaMP-EF15

    Plasmid
    #52641
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-EF15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D431N/D433N/D435N mutated from GCaMP3
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. GFP-FKBP-LBR

    Plasmid
    #222427
    Purpose
    To express LBR with N-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    LBR (LBR Human)
    Use
    Tags
    GFP-FKBP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    222427-plasmid-map-sequence-id-439259

    Enlarge

  45. LBR(R583Q)-FKBP-GFP

    Plasmid
    #222423
    Purpose
    To express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    LBR(R583Q) (LBR Human)
    Use
    Tags
    FKBP-GFP
    Expression
    Mammalian
    Mutation
    R583Q
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222423-plasmid-map-sequence-id-439219

    Enlarge

  46. LBR(N547D)-FKBP-GFP

    Plasmid
    #222422
    Purpose
    To express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    LBR(N547D) (LBR Human)
    Use
    Tags
    FKBP-GFP
    Expression
    Mammalian
    Mutation
    N547D
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222422-plasmid-map-sequence-id-439218

    Enlarge

Showing: 951 - 1000 of 2950 results