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Showing: 10051 - 10100 of 12274 results

  2. pVH1002-Tier2(pUC ori)

    Plasmid
    #169600
    Purpose
    Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169600-plasmid-map-sequence-id-366610

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  3. ECFP HO

    Plasmid
    #207413
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207413-plasmid-map-sequence-id-421043

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  4. ECFP CD

    Plasmid
    #207414
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207414-plasmid-map-sequence-id-421036

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  7. PXWABS62(E11-TEV)

    Plasmid
    #123157
    Purpose
    For TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ParsR-ABS62, Pecf11
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123157-plasmid-map-sequence-id-243988

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  10. pDP085

    Plasmid
    #198425
    Purpose
    pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120
    Depositor
    Markus Schmid
    Article
    You et al Nat Commun. 2017 May 17;8:15120. doi: 10
    Insert
    E. coli BirA
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pUBQ10
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    198425-plasmid-map-sequence-id-388239

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  17. pcDNA3-InPAkt

    Plasmid
    #181914
    Purpose
    Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.
    Depositor
    Jin Zhang
    Article
    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10
    Insert
    InPAkt
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181914-plasmid-map-sequence-id-363357

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  20. pRSET a sfGCaMP6s

    Plasmid
    #100022
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100022-plasmid-map-sequence-id-194291

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  22. pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272

    Plasmid
    #154829
    Purpose
    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietin
    Depositor
    Lise Marie Grav
    Article
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    coEPO
    coEPO
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized (Cricetulus griseus)
    Promoter
    100RPU.2
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154829-plasmid-map-sequence-id-308567

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  23. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

    Plasmid
    #169531
    Purpose
    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169531-plasmid-map-sequence-id-364563

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  24. pXWJ109AsAmp30E

    Plasmid
    #123144
    Purpose
    Arsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, ParsR, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123144-plasmid-map-sequence-id-240606

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  26. shNTC

    Plasmid
    #73549
    Purpose
    Non-targeting control shRNA cloned in retroviral vector pMLP (MSCV-based vector expressing shRNA in a mir30 context).
    Depositor
    Gerardo Ferbeyre
    Article
    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d
    Insert
    shNTC
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Aug. 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. pXWJ109HgAmp30E

    Plasmid
    #123149
    Purpose
    Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, PmerT, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123149-plasmid-map-sequence-id-244017

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  30. pTS2356_Tier3(PB)-PuroR

    Plasmid
    #169655
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169655-plasmid-map-sequence-id-364560

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  33. pYFAC-CH1

    Plasmid
    #168977
    Purpose
    AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in fil…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    168977-plasmid-map-sequence-id-359562

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  34. pACEC3GH (Apr)

    Plasmid
    #110085
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110085-plasmid-map-sequence-id-211188

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  35. pSSA-PMG (pXK-1649)

    Plasmid
    #208857
    Purpose
    SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.
    Depositor
    Kun Xu
    Article
    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208857-plasmid-map-sequence-id-417238

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  37. pVH617

    Plasmid
    #169606
    Purpose
    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (A1-pA::PmPGK1-Fluc-pA::PCREm4-Nluc-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PCREm4 / PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169606-plasmid-map-sequence-id-365600

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  41. pLaNGo11-1

    Plasmid
    #126053
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126053-plasmid-map-sequence-id-255676

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  45. pTS2311-Tier1-spacer-PPGK-NLuc_TB

    Plasmid
    #169552
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-NanoLuc Luciferase - mTagBFP fusion
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169552-plasmid-map-sequence-id-364979

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  46. pTS2381-Tier1-PhCMV-Igk_TM

    Plasmid
    #169487
    Purpose
    Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven membrane localization
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV
    Available since
    June 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    169487-plasmid-map-sequence-id-364214

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  47. pVH615

    Plasmid
    #169604
    Purpose
    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (A1-pA::PCREm4-Nluc-pA::PmPGK1-Fluc-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PCREm4 / PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169604-plasmid-map-sequence-id-366613

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  49. pcDNA3-ABKAR-Kras

    Plasmid
    #84637
    Purpose
    Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, C…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    84637-plasmid-map-sequence-id-176126

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Showing: 10051 - 10100 of 12274 results