Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

179,521 results

Showing: 8081 - 8120 of 179521 results

  2. Myr-(iSH2-p85)-p110alpha-Myc

    Plasmid
    #1410
    Depositor
    Lewis Cantley
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    SRalpha promoter
    Available since
    June 16, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1410-plasmid-map-sequence-id-352163

    Enlarge

  5. pJSK664

    Plasmid
    #205130
    Purpose
    Expression of GFP-2xPX (from SnxA) in mammalian cells
    Depositor
    Jason King
    Publication
    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tandem 2xPX domain of SnxA
    Tags
    EGFP
    Expression
    Mammalian
    Available since
    Sept. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    205130-plasmid-map-sequence-id-408467

    Enlarge

  9. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid
    #188899
    Purpose
    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Zim3-dCas9
    Use
    Lentiviral
    Tags
    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion
    Promoter
    SFFV
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    188899-plasmid-map-sequence-id-374831

    Enlarge

  10. Epac-S-H187

    Plasmid
    #170348
    Purpose
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170348-plasmid-map-sequence-id-336137

    Enlarge

  15. eGFP-ova-C1

    Plasmid
    #163524
    Purpose
    Expresses both eGFP and chicken ovalbumin in mammalian cells
    Depositor
    Thomas Weber
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enhanced-GFP-chicken ovalbumin tandem
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    163524-plasmid-map-sequence-id-321577

    Enlarge

  17. pSL1425 (pSPIN, pCDF backbone)

    Plasmid
    #160735
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.
    Depositor
    Samuel H. Sternberg
    Publication
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    160735-plasmid-map-sequence-id-317231

    Enlarge

  21. pET_RTX(exo-)-6xHis

    Plasmid
    #145028
    Purpose
    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag
    Depositor
    Andrew Ellington
    Publication
    Bhadra et al BioProtocol 2021
    Insert
    RTX(exo-)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    N210D
    Promoter
    T7
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145028-plasmid-map-sequence-id-292638

    Enlarge

  22. pGBW-m4046887

    Plasmid
    #145730
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    145730-plasmid-map-sequence-id-292497

    Enlarge

  23. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid
    #140005
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140005-plasmid-map-sequence-id-275488

    Enlarge

Showing: 8081 - 8120 of 179521 results