159,481 results

1. pMRE-Tn7-141

   Plasmid

   #118557

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCI-SEP-GluR1

   Plasmid

   #24000

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   GluR1 (Gria1 Rat)
   

   Use

   Tags

   GluR1 signal peptide (aa1-22) and SEP (supereclip…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FLAG-NM23-H1

   Plasmid

   #25000

   Depositor

   Judy Lieberman

   Insert

   NM23-H1 (NME1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pET28a_Pro-IL-33

   Plasmid

   #214342

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL33 (IL33 Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. V48 ELP

   Plasmid

   #68395

   Purpose

   Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

   Depositor

   Andrew MacKay

   Article

   Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

   Insert

   V48 ELP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

   Plasmid

   #216164

   Purpose

   Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   Gaussia princeps luciferase (with double mutation to improve stability)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

   Plasmid

   #225708

   Purpose

   Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

   Depositor

   Michael Lin

   Article

   Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

   Insert

   ASAP5-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1α

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-P3-evoCjCas9-ABE8e_gRNA

   Plasmid

   #202559

   Purpose

   P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-ABE8e
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOTTC1484 - pAAV SYN1 HA-hM4D(Gi)

   Plasmid

   #121538

   Purpose

   An adeno-associated viral vector expressing HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

   Depositor

   Christopher Richie

   Article

   Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

   Has Service

   AAV2 and AAV5

   Insert

   HA-tagged hM4D(Gi) (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SYN1

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-cT3G-cERP2-RUNX1

    Plasmid

    #192928

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of RUNX1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pP450cam

    Plasmid

    #85085

    Purpose

    Expresses P450cam (CYP101A1) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    P450cam
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSM-GFPnovo2

    Plasmid

    #116943

    Purpose

    GFPnovo2_unc-54 utr vector for expression in C. elegans

    Depositor

    Kota Mizumoto

    Article

    Hendi et al MicroPubl Biol. 2018 Sep 6;2018:10.17912

    Insert

    C. elegans codon optimized GFPnovo2
    

    Use

    Tags

    GFPnovo2

    Expression

    Worm

    Mutation

    Y145F, V163A, S202T, L221V

    Promoter

    none

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. VEGFR2-mCh

    Plasmid

    #108854

    Purpose

    Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

    Depositor

    Kalina Hristova

    Article

    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Labeled with mCherry on the C-Terminus via a 3 am…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-hSyn-GRAB_NE1h

    Plasmid

    #123309

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE sensor GRAB_NE1h
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pARF1-CFP

    Plasmid

    #11381

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 1 (ARF1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-CalfluxCTN

    Plasmid

    #190068

    Purpose

    Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

    Depositor

    Carl Johnson

    Article

    Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

    Insert

    CalfluxCTN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRBV20-1

    Plasmid

    #123454

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV20-1 (TRBV20-1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR_TRE3GS-->mCherry_PGK-->BFP

    Plasmid

    #162231

    Purpose

    Lentiviral vector for the expression of TRE3GS-->mCherry_PGK-->BFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    mCherry and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF0060

    Plasmid

    #141501

    Purpose

    Lentiviral vector for overexpressing the CTBP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CTBP2 (CTBP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-SET

    Plasmid

    #234708

    Purpose

    expresses His-tagged human SET in E coli

    Depositor

    Lienhard Schmitz

    Article

    Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

    Insert

    SET (SET Human)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPB-cT3G-cERP2-GATA4

    Plasmid

    #192896

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Codon-optimized

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR223 SARS-CoV-2 NSP12

    Plasmid

    #141265

    Purpose

    Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Gene ID 43740578)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. 5'UTR CGG 99x FMR1-EGFP

    Plasmid

    #63091

    Purpose

    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC

    Plasmid

    #52985

    Purpose

    split Venus attached to MCP and PCP respectively

    Depositor

    Robert Singer

    Article

    Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

    Insert

    nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    ubc

    Available since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CFP-Parkin

    Plasmid

    #47560

    Purpose

    CFP tagged Parkin for colocalization study

    Depositor

    Richard Youle

    Article

    Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSAM2_mCherry_Tbx20

    Plasmid

    #72697

    Purpose

    Doxycyline regulated expression of Tbx20 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Tbx20 (Tbx20 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mCherry-Lrrc59

    Plasmid

    #226565

    Purpose

    Encodes Lrrc59 protein laebeled with mCherry

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MSCV

    Plasmid

    #24828

    Depositor

    Lin He

    Article

    Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMIAII-Cd4

    Plasmid

    #194401

    Purpose

    Retroviral expression of mouse CD4

    Depositor

    Michael Carroll

    Article

    Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

    Insert

    Cd4 (Cd4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. N174-MCS

    Plasmid

    #81061

    Purpose

    Lentiviral vector for constitutive gene expression.

    Depositor

    Adam Karpf

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLOC-MCOLN1

    Plasmid

    #131583

    Purpose

    For expression of human MCOLN1 in mammalian cells, GFP-tagged

    Depositor

    Sean Morrison

    Article

    Kasitinon et al Cell Rep. 2019 Aug 27;28(9):2293-2305.e9

    Insert

    MCOLN1 (MCOLN1 Human)
    

    Use

    Lentiviral

    Tags

    turboGFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 8522-M01-663

    Plasmid

    #225660

    Purpose

    Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCopGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SORE6-mCMVp

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCRISPaint-mNeon

    Plasmid

    #174092

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    mNeonGreen
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pmIL-6 FL

    Plasmid

    #61286

    Purpose

    drives luciferase from Wt mouse IL-6 promoter (full length)

    Depositor

    Gail Bishop

    Article

    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

    Insert

    IL-6 promoter (Il6 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    IL-6 full length promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-Neo-Bam APC 1-1941

    Plasmid

    #16510

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    APC (APC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon 1941 truncation

    Promoter

    Available since

    April 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJ2072.2_CRISPRlator

    Plasmid

    #140674

    Purpose

    CRISPRlator

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    CRISPRlator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAJM.657

    Plasmid

    #108525

    Purpose

    CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    CymRAM + PCymRC-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    CymR: S110G A171V

    Promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLX401-INK4A

    Plasmid

    #121919

    Purpose

    Doxycycline-inducible expression of p16-INK4A product of CDKN2A

    Depositor

    William Hahn

    Article

    Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

    Insert

    p16-INK4A (CDKN2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Tet ON

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSF278

    Plasmid

    #167673

    Purpose

    Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Lenti_gRNA-Puro

    Plasmid

    #84752

    Purpose

    Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge