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Showing: 10251 - 10300 of 12274 results

  4. pYFAC-CH4

    Plasmid
    #168980
    Purpose
    AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    168980-plasmid-map-sequence-id-350001

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  8. pNAD1

    Plasmid
    #154347
    Purpose
    pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance
    Depositor
    John van der Oost
    Article
    Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.
    Insert
    ShBle
    Use
    Microalgae
    Tags
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Violaxanthin Chlorophyll a Binding Protein Precur…
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154347-plasmid-map-sequence-id-316607

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  9. pYFAC-CH2

    Plasmid
    #168978
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    168978-plasmid-map-sequence-id-353766

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  19. pXWJ109As(RS-te-AAV)

    Plasmid
    #123156
    Purpose
    For TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, ParsR, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123156-plasmid-map-sequence-id-240600

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  23. pYFAC-CH3

    Plasmid
    #168979
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    168979-plasmid-map-sequence-id-350204

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  25. pAAV-UbC-mEmerald Rab7a

    Plasmid
    #115238
    Purpose
    To express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.
    Depositor
    Pantelis Tsoulfas
    Insert
    Rab7a fused to mEmerald (RAB7A Human)
    Use
    AAV
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    7 Amino Acids (21 Nucleotides) between mEmerald a…
    Promoter
    Human UbC
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    115238-plasmid-map-sequence-id-222080

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  26. LSB-hsa-miR-941

    Plasmid
    #103757
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-941 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-941 target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103757-plasmid-map-sequence-id-196562

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  28. pcDNA3.1(+)-FluoSTEP-AktAR

    Plasmid
    #181856
    Purpose
    Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Insert
    FluoSTEP-AktAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181856-plasmid-map-sequence-id-362780

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  29. pJAK080

    Plasmid
    #167279
    Purpose
    CodA-based vector for insertion of DNA by recombination downstream of the pyrE gene in C. difficile
    Depositor
    Robert Fagan
    Article
    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11
    Type
    Empty backbone
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    167279-plasmid-map-sequence-id-330742

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  33. pBMN ltDHFR(DD)-YFP

    Plasmid
    #47076
    Purpose
    ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans
    Depositor
    Thomas Wandless
    Article
    Cho et al PLoS One. 2013 Aug 22;8(8):e72393. doi:
    Insert
    ltDHFR(DD)
    Use
    Retroviral
    Tags
    HA and YFP
    Expression
    Mutation
    amino acids mutations: R12H, N23S, G67S, V78A, E1…
    Promoter
    LTR
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47076-plasmid-map-sequence-id-67102

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  34. pRSET a MatryoshCaMP6s

    Plasmid
    #100019
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    MatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100019-plasmid-map-sequence-id-194295

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  36. pBMN YFP-ltDHFR(DD)

    Plasmid
    #47080
    Purpose
    ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans
    Depositor
    Thomas Wandless
    Article
    Cho et al PLoS One. 2013 Aug 22;8(8):e72393. doi:
    Insert
    YFP-ltDHFR(DD)
    Use
    Retroviral
    Tags
    HA and YFP
    Expression
    Mutation
    amino acids mutations: R12H, N37D, N59D, G67S, D1…
    Promoter
    LTR
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47080-plasmid-map-sequence-id-67106

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  40. Clover-pBAD

    Plasmid
    #54575
    Purpose
    Localization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.
    Depositor
    Michael Davidson
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Type
    Empty backbone
    Use
    Tags
    6xHis and Clover
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  41. BII-gR-BtW-eSpCas9

    Plasmid
    #133358
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
    Depositor
    Blair Madison
    Article
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Insert
    eSpCas9
    Use
    Tags
    Flag-tagged eSpCas9
    Expression
    Mammalian
    Mutation
    eSpCas9 mutations
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    133358-plasmid-map-sequence-id-269429

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  47. pTS2337_Tier3(SB)-TagBFP2_Puro

    Plasmid
    #169635
    Purpose
    Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mTagBFP2 and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169635-plasmid-map-sequence-id-366786

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  50. pCYC1pr-GNSI (LEU)

    Plasmid
    #111450
    Purpose
    GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    111450-plasmid-map-sequence-id-211605

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Showing: 10251 - 10300 of 12274 results